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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
2533 results for " His Active" - showing 100-150
Activity Data
(Figure. The binding activity of tPA with PAI1.Plasminogen activators are serine proteases that catalyze the activation of plasmin via proteolytic cleavage of its zymogen form plasminogen. Plasmin is an important factor in fibrinolysis, the breakdown of fibrin polymers formed during blood clotting. There are two main plasminogen activators: urokinase (uPA) and tissue plasminogen activator (tPA).Tissue plasminogen activators (tPA) are used to treat medical conditions related to blood clotting including embolic or thrombotic stroke, myocardial infarction, and pulmonary embolism. Besides, Plasminogen Activator Inhibitor 1 (PAI1) has been identified as an interactor of tPA, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse tPA and recombinant mouse PAI1. Briefly, tPA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PAI1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-tPA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of tPA and PAI1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Plasminogen Activator, Tissue (tPA), Active Protein (Cat# AAA2105096)
Full Name
Active Plasminogen Activator, Tissue (tPA)
Gene Names
Plat; tPA; AU020998; AW212668; D8Ertd2e
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$245/0.01 mg | $530/0.05 mg | $820/0.1 mg | $1,020/0.2 mg | $1,985/0.5 mg | $2,945/1 mg | $7,200/5 mg
Activity Data
(TF (Tissue factor) also known for platelet tissue factor, factor III, thromboplastin, or CD142, is a cell surface glyprotein present in subendothelial tissue and leukocytes. TF is necessary for the initiation of thrombin formation from the zymogen prothrombin. It has been proven that EGF can bind with the TF on the hemangioendotheliocytes. Thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TF and recombinant rat EGF. Briefly, TF were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TF were then transferred to EGF-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TF and EGF was shown in Figure 1, and this effect was in a dose dependent manner.)
Tissue Factor (TF), Active Protein (Cat# AAA2097277)
Full Name
Active Tissue Factor (TF)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$255/0.01 mg | $575/0.05 mg | $895/0.1 mg | $1,100/0.2 mg | $2,170/0.5 mg | $3,210/1 mg | $7,865/5 mg
Bone Morphogenetic Protein 7 (BMP7), Active Protein (Cat# AAA2086082)
Full Name
Active Bone Morphogenetic Protein 7 (BMP7)
Gene Names
Bmp7; OP1
Reactivity
Mus musculus (Mouse)
Applications
Cell culture, Activity Assays
Purity
>98%
Pricing
$215/0.01 mg | $460/0.05 mg | $700/0.1 mg | $870/0.2 mg | $1,695/0.5 mg | $2,520/1 mg | $6,165/5 mg
Activity Data
(Figure. The binding activity of ACE with ACTb.Angiotensin-converting enzyme ACE, is a central component of the renin-angiotensin system (RAS), which controls blood pressure by regulating the volume of fluids in the body. It converts the hormone angiotensin I to the active vasoconstrictor angiotensin II. Therefore, ACE indirectly increases blood pressure by causing blood vessels to constrict. ACE inhibitors are widely used as pharmaceutical drugs for treatment of cardiovascular diseases. Besides, Actin Beta (ACTb) has been identified as an interactor of ACE, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ACE and recombinant human ACTb. Briefly, ACE were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ACE pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ACE and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.)
Angiotensin I Converting Enzyme (ACE), Active Protein (Cat# AAA2105044)
Full Name
Active Angiotensin I Converting Enzyme (ACE)
Gene Names
ACE; DCP; ACE1; DCP1; CD143
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$225/0.01 mg | $480/0.05 mg | $745/0.1 mg | $920/0.2 mg | $1,795/0.5 mg | $2,660/1 mg | $6,500/5 mg
Superoxide Dismutase, Active Protein (Cat# AAA144976)
Full Name
Recombinant Human Superoxide Dismutase His Tag
Gene Names
SOD1; ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer
Purity
Greater than 95.0% as determined by SDS-PAGE and HPLC analyses.
Pricing
$180/0.02 mg | $250/0.1 mg | $710/1 mg | $2,975/5x1 mg
Sequence
Adiponectin Receptor 1 (ADIPOR1), Active Protein (Cat# AAA2097251)
Full Name
Active Adiponectin Receptor 1 (ADIPOR1)
Gene Names
ADIPOR1; CGI45; PAQR1; ACDCR1; CGI-45; TESBP1A
Reactivity
Homo sapiens (Human)
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
>98%
Pricing
$185/0.01 mg | $360/0.05 mg | $550/0.1 mg | $675/0.2 mg | $1,310/0.5 mg | $1,950/1 mg | $4,785/5 mg
Activity Data
(JAK2 (Tyrosine-protein kinase JAK2) is a tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. JAK2 is considered to associate with some type I receptors, including EPOR (Erythropoietin receptor), therefore participates in cellular signal transduction. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human JAK2 and recombinant human EPOR. Briefly, JAK2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL JAK2 were then transferred to EPOR-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-JAK2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of JAK2 and EPOR was shown in Figure 1, and this effect was in a dose dependent manner.)
Janus Kinase 2 (JAK2), Active Protein (Cat# AAA2097244)
Full Name
Active Janus Kinase 2 (JAK2)
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$205/0.01 mg | $420/0.05 mg | $640/0.1 mg | $795/0.2 mg | $1,540/0.5 mg | $2,295/1 mg | $5,605/5 mg
Activity Data
(Figure. The binding activity of ODC with TK1.Ornithine decarboxylase (ODC) is an enzyme can catalyze the decarboxylation of ornithine (a product of the urea cycle) to form putrescine. The ornithine decarboxylation reaction catalyzed by ornithine decarboxylase is the first and committed step in the synthesis of polyamines, particularly putrescine, spermidine and spermine. Polyamines are important for stabilizing DNA structure, the DNA double strand-break repair pathway and as antioxidants. Therefore, ornithine decarboxylase is an essential enzyme for cell growth, producing the polyamines necessary to stabilize newly synthesized DNA. Lack of ODC causes cell apoptosis in embryonic mice, induced by DNA damage. Besides, Thymidine Kinase 1, Soluble (TK1) has been identified as an interactor of ODC, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ODC and recombinant human TK1. Briefly, ODC were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to TK1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ODC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ODC and TK1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Ornithine Decarboxylase (ODC), Active Protein (Cat# AAA2105136)
Full Name
Active Ornithine Decarboxylase (ODC)
Gene Names
ODC1; ODC
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$225/0.01 mg | $490/0.05 mg | $755/0.1 mg | $940/0.2 mg | $1,830/0.5 mg | $2,710/1 mg | $6,620/5 mg
Sequence
Myxovirus Resistance 1 (MX1), Active Protein (Cat# AAA2105192)
Full Name
Active Myxovirus Resistance 1 (MX1)
Gene Names
MX1; MX; MxA; IFI78; IFI-78K
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>98%
Pricing
$205/0.01 mg | $420/0.05 mg | $640/0.1 mg | $790/0.2 mg | $1,535/0.5 mg | $2,290/1 mg | $5,590/5 mg
Sequence
Mdm2 p53 Binding Protein Homolog (MDM2), Active Protein (Cat# AAA2097320)
Full Name
Active Mdm2 p53 Binding Protein Homolog (MDM2)
Gene Names
MDM2; HDMX; hdm2; ACTFS
Applications
Cell culture; Activity Assays.
Purity
>95%
Pricing
$225/0.01 mg | $490/0.05 mg | $755/0.1 mg | $940/0.2 mg | $1,830/0.5 mg | $2,710/1 mg | $6,620/5 mg
Activity Data
(Golgi Protein 73 (GP73) also known as Golgi phosphoprotein 2 or Golgi membrane protein GP73 is a protein that encoded by the GOLM1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. It has been observed to be upregulated in response to viral infection. Besides, Dymeclin (DYM) has been identified as an interactor of GP73, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse GP73 and recombinant human DYM. Briefly, GP73 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to DYM-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GP73 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of GP73 and DYM was shown in Figure 1, and this effect was in a dose dependent manner.)
Golgi Protein 73 (GP73), Active Protein (Cat# AAA2097297)
Full Name
Active Golgi Protein 73 (GP73)
Gene Names
Golm1; GP73; Golph2; AW125446; PSEC0257; 2310001L02Rik; D030064E01Rik
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$265/0.01 mg | $595/0.05 mg | $920/0.1 mg | $1,135/0.2 mg | $2,240/0.5 mg | $3,315/1 mg | $8,120/5 mg
IDO, Active Protein (Cat# AAA637936)
Full Name
IDO, Recombinant, Mouse (His) (Indoleamine 2,3-dioxygenase, INDO)
Gene Names
IDO1; IDO; INDO; IDO-1
Purity
Highly Purified
>=90% (SDS-PAGE)
>=90% (SDS-PAGE)
Pricing
$680/0.05 mg | $3,060/5x0.05 mg
Sequence Information
Secretogranin II (SCG2), Recombinant Protein (Cat# AAA2012411)
Full Name
Recombinant Secretogranin II (SCG2)
Gene Names
Scg2; Chgc; SgII
Reactivity
Mus musculus (Mouse)
Applications
WB
Purity
> 95%
Pricing
$165/0.01 mg | $305/0.05 mg | $460/0.1 mg | $565/0.2 mg | $1,085/0.5 mg | $1,605/1 mg | $3,935/5 mg
Sequence
Interleukin 1 Receptor Antagonist (IL1RA), Active Protein (Cat# AAA2097334)
Full Name
Active Interleukin 1 Receptor Antagonist (IL1RA)
Gene Names
Il1rn; IL-1ra; F630041P17Rik
Applications
Cell culture; Activity Assays; In vivo assays.
Purity
>95%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
Activity Data
(Figure. The binding activity of RANKL with PPARg.Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is a protein that in humans is encoded by the TNFSF11 gene. RANKL is a member of the tumor necrosis factor (TNF) cytokine family, it binds to RANK on cells of the myeloid lineage and functions as a key factor for osteoclast differentiation and activation. It may also bind to osteoprotegerin, a protein secreted mainly by cells of the osteoblast lineage which is a potent inhibitor of osteoclast formation by preventing binding of RANKL to RANK. RANKL also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. This protein was shown to be a dendritic cell survival factor and is involved in the regulation of T cell-dependent immune response. Besides, Peroxisome Proliferator Activated Receptor Gamma (PPARg) has been identified as an interactor of RANKL, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse RANKL and recombinant mouse PPARg. Briefly, RANKL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PPARg-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-RANKL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of RANKLand PPARg was shown in Figure 1, and this effect was in a dose dependent manner.)
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL), Active Protein (Cat# AAA2105122)
Full Name
Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
Gene Names
Tnfsf11; ODF; OPGL; RANKL; Ly109l; Trance
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$225/0.01 mg | $490/0.05 mg | $755/0.1 mg | $940/0.2 mg | $1,830/0.5 mg | $2,710/1 mg | $6,620/5 mg
Activity Data
(Matrix Metalloproteinase 13 (MMP13) is a member of the matrix metalloproteinase (MMP) family. MMP13 has been proposed to participate in aggrecan degradation associated with osteoarthritis and cleavage of type II collagen in osteoarthritic cartilage explants and in tumor progression and metastasis. In addition, it can cleave type I, III, IV, IX, X and XIV collagens and fibronectin. MMP13 is likely to play a crucial role in the modulation of extracellular matrix degradation and cell-matrix interactions. Although gelatin zymography is mainly used for the detection of the MMP2 and MMP9, it also can be used for MMP13 dection. Briefly, various concentrations of MMP13 (1000ng, 500ng, 250ng, 125ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphat- polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP13 would hydrolyze gelatin nearby, which was indicated by the white bands on the gel. In this experiment we use heat-denatured MMP13 protein as negative control, and blood sample as positive control. Result: Gelatin hydrolysis by recombinant rat MMP13 was shown in figure 1.)
Matrix Metalloproteinase 13 (MMP13), Active Protein (Cat# AAA2097264)
Full Name
Active Matrix Metalloproteinase 13 (MMP13)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$265/0.01 mg | $595/0.05 mg | $920/0.1 mg | $1,135/0.2 mg | $2,240/0.5 mg | $3,315/1 mg | $8,120/5 mg
SDS-Page
Galectin-3, Active Protein (Cat# AAA203603)
Full Name
Galectin-3, 1-250aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
$200/0.02 mg | $315/0.1 mg | $680/0.5 mg | $2,820/5x0.5 mg
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized fby coomassie blue stain.)
Peroxiredoxin 6, Active Protein (Cat# AAA203357)
Full Name
Peroxiredoxin 6, 1-224aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
PRDX6; PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; HEL-S-128m
Applications
Enzyme Activity, SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
$215/0.02 mg | $340/0.1 mg | $765/0.5 mg | $3,195/5x0.5 mg
Activity Data
(Figure. The binding activity of OC with FUR.Osteocalcin (OC), also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a noncollagenous protein hormone found in bone and dentin. Osteocalcin is secreted solely by osteoblasts and thought to play a role in the body's metabolic regulation and is pro-osteoblastic, or bone-building, by nature. It is also implicated in bone mineralization and calcium ion homeostasis. Osteocalcin acts as a hormone in the body, causing beta cells in the pancreas to release more insulin, and at the same time directing fat cells to release the hormone adiponectin, which increases sensitivity to insulin. Besides, Furin (FUR) has been identified as an interactor of OC, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse OC and recombinant mouse FUR. Briefly, OC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100mul were then transferred to FUR-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-OC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of OC and FUR was shown in Figure 1, and this effect was in a dose dependent manner.)
Osteocalcin (OC), Active Protein (Cat# AAA2105093)
Full Name
Active Osteocalcin (OC)
Gene Names
Bglap; OC; BGP; OG1; mOC-A; Bglap1
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$225/0.01 mg | $490/0.05 mg | $755/0.1 mg | $940/0.2 mg | $1,830/0.5 mg | $2,710/1 mg | $6,620/5 mg
Glucagon, Active Protein (Cat# AAA142631)
Full Name
Recombinant Human Glucagon
Gene Names
GCG; GLP1; GLP2; GRPP
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Pricing
$180/0.1 mg | $250/0.5 mg | $355/1 mg | $1,375/5x1 mg
Activity Data
(Figure. The binding activity of FGF21 with FGFR1.Fibroblast growth factor 21 (FGF21) is a member of the fibroblast growth factor (FGF) family and specifically a member of the endocrine subfamily which includes FGF23 and FGF15/19. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 action through one of the FGF21 receptors thus requires interaction with a co-receptor, designated beta-klotho. Besides, Fibroblast Growth Factor Receptor 1 (FGFR1) has been identified as an interactor of FGF21, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat FGF21 and recombinant rat FGFR1. Briefly, FGF21 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to FGFR1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FGF21 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of FGF21 and FGFR1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Fibroblast Growth Factor 21 (FGF21), Active Protein (Cat# AAA2105177)
Full Name
Active Fibroblast Growth Factor 21 (FGF21)
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$235/0.01 mg | $510/0.05 mg | $790/0.1 mg | $980/0.2 mg | $1,915/0.5 mg | $2,830/1 mg | $6,925/5 mg
Activity Data
(Figure. The binding activity of BDNF with APP.Brain-derived neurotrophic factor, also known as BDNF, is a member of the neurotrophin family of growth factors, which are related to the canonical Nerve Growth Factor. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. Besides, Amyloid Precursor Protein (APP) has been identified as an interactor of BDNF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse BDNF and recombinant mouse APP. Briefly, BDNF were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to APP-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-BDNF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of BDNF and APP was shown in Figure 1, and this effect was in a dose dependent manner.)
Brain Derived Neurotrophic Factor (BDNF), Active Protein (Cat# AAA2105047)
Full Name
Active Brain Derived Neurotrophic Factor (BDNF)
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$250/0.01 mg | $540/0.05 mg | $840/0.1 mg | $1,045/0.2 mg | $2,040/0.5 mg | $3,020/1 mg | $7,385/5 mg
SDS-Page
(SDS-PAGE of 78kDa Grp78 (Bip) protein (SPR-107).)
Grp78 (Bip), Active Protein (Cat# AAA806942)
Full Name
Recombinant Human GRP78 (Bip)
Gene Names
HSPA5; BIP; MIF2; GRP78; HEL-S-89n
Applications
WB, EIA
Purity
Affinity Purified
Pricing
$265/0.05 mg | $380/0.1 mg | $2,640/10x0.1 mg | $1,490/5x0.1 mg | $980/5x0.05 mg | $640/2x0.1 mg
Sequence
Metallothionein 1 (MT1), Recombinant Protein (Cat# AAA2012140)
Full Name
Recombinant Metallothionein 1 (MT1)
Gene Names
MT1A; MT1; MTC; MT1S
Reactivity
Homo sapiens (Human)
Applications
WB
Purity
> 97%
Pricing
$155/0.01 mg | $280/0.05 mg | $425/0.1 mg | $515/0.2 mg | $990/0.5 mg | $1,460/1 mg | $3,575/5 mg | $16,080/5x5 mg
Activity Data
(ARSF (Arylsulfatase F) is a member of the sulfatase gene family, and more specifically, the arylsulfatase subfamily. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Besides, EP300 (E1A binding protein p300) has been identified as an interactor of ARSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSF and recombinant human EP300. Briefly, ARSF were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EP300-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ARSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of ARSF and EP300 was shown in Figure 1, and this effect was in a dose dependent manner.)
Arylsulfatase F (ARSF), Active Protein (Cat# AAA2097319)
Full Name
Active Arylsulfatase F (ARSF)
Gene Names
ARSF; ASF
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$255/0.01 mg | $575/0.05 mg | $895/0.1 mg | $1,100/0.2 mg | $2,170/0.5 mg | $3,210/1 mg | $7,865/5 mg
Activity Data
(Figure. The binding activity of AHCY with MHCB.Adenosylhomocysteinase (S-adenosylhomocysteine synthase, S-adenosylhomocysteine hydrolase, adenosylhomocysteine hydrolase, S-adenosylhomocysteinase, SAHase, AdoHcyase, AHCY) is an enzyme that converts S-adenosylhomocysteine to homocysteine and adenosine. The enzyme contains one tightly bound NAD per subunit. Besides, Major Histocompatibility Complex Class I B (MHCB) has been identified as an interactor of AHCY, thus a binding ELISA assay was conducted to detect the interaction of recombinant human AHCY and recombinant human MHCB. Briefly, AHCY were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCB-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-AHCY pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of AHCY and MHCB was shown in Figure 1, and this effect was in a dose dependent manner.)
Adenosylhomocysteinase (AHCY), Active Protein (Cat# AAA2105187)
Full Name
Active Adenosylhomocysteinase (AHCY)
Gene Names
AHCY; SAHH; adoHcyase
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$235/0.01 mg | $510/0.05 mg | $790/0.1 mg | $980/0.2 mg | $1,915/0.5 mg | $2,830/1 mg | $6,925/5 mg
Activity Data
(Figure. The binding activity of FGF9 with FGFR1.Fibroblast Growth Factor 9 (FGF9) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF9 was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Besides, Fibroblast Growth Factor Receptor 1 (FGFR1) has been identified as an interactor of FGF6, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF9 and recombinant human FGFR1. Briefly, FGF9 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FGFR1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FGF9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of FGF9 and FGFR1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Fibroblast Growth Factor 9 (FGF9), Active Protein (Cat# AAA2105055)
Full Name
Active Fibroblast Growth Factor 9 (FGF9)
Gene Names
FGF9; GAF; FGF-9; SYNS3; HBFG-9; HBGF-9
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$155/0.01 mg | $285/0.05 mg | $430/0.1 mg | $525/0.2 mg | $1,010/0.5 mg | $1,490/1 mg | $3,645/5 mg | $16,390/5x5 mg
Activity Data
(PTH (Parathyroid hormone) is a hormone secreted by the parathyroid glands that is important in bone remodeling. As reported, osteoblast-like cell lines, such as ROS 17/2.8, UMR106, SaOS, U2OS, MG63, that exhibit PTHR1, respond with increased proliferation to PTH. Rat PTH shares similarities with human PTH in amino acid sequence with the identity of 71.3%. Thus, a proliferation assay of rat recombinant PTH was conducted using U2OS cells. Briefly, U2OS cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of PTH. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of U2OS cells after incubation with PTH for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant PTH for 48h. The result was shown in Figure 2. It was obvious that PTH increased cell viability of U2OS cells.)
Parathyroid Hormone (PTH), Active Protein (Cat# AAA2097252)
Full Name
Active Parathyroid Hormone (PTH)
Gene Names
Pth; Pth1; Pthr1; PTH-(1-84)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$175/0.01 mg | $330/0.05 mg | $505/0.1 mg | $620/0.2 mg | $1,195/0.5 mg | $1,775/1 mg | $4,350/5 mg
Sequence
Histidine Rich Glycoprotein (HRG), Active Protein (Cat# AAA2086087)
Full Name
Active Histidine Rich Glycoprotein (HRG)
Gene Names
HRG; HPRG; HRGP; THPH11
Reactivity
Homo sapiens (Human)
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
>98%
Pricing
$180/0.01 mg | $345/0.05 mg | $530/0.1 mg | $650/0.2 mg | $1,260/0.5 mg | $1,870/1 mg | $4,595/5 mg
Activity Data
(Figure 1. Cell apoptosis of A549 cells after stimulated by TNFa. TNFa, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, inflammation and inhibit tumorigenesis. As reported, TNFa could inhibit the proliferation and induce apoptosis of A549 cells, and the concentration of IL-1beta in cell supernatant will increase after stimulation. A549 cells were incubated in DMEM with TNFa (1ng/mL, 10ng/mL) for 2h, 4h, 8h, 24h, 48h, then cells were observed by inverted microscope and IL-1beta in cell supernatant was detected by ELISA. Cell apoptosis of A549 after incubation of 48h was shown in Figure 1. (A)A549 cells cultured in DMEM, stimulated with 1ng/mL TNFa for 48h; (B)A549 cells cultured in DMEM, stimulated with 10ng/mL TNFa for 48h; (C)A549 cells cultured in DMEM for 48h.)
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA2086072)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Gene Names
Tnf; DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$175/0.01 mg | $330/0.05 mg | $505/0.1 mg | $620/0.2 mg | $1,195/0.5 mg | $1,775/1 mg | $4,350/5 mg
SDS-Page
GST, Active Protein (Cat# AAA203606)
Full Name
GST, 1-218aa, Schistosoma japonicum, His tag, E Coli (Bioactivity Validated)
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
$200/0.02 mg | $315/0.1 mg | $680/0.5 mg | $2,820/5x0.5 mg
SDS-Page
HPRT, Active Protein (Cat# AAA206473)
Full Name
HPRT, 1-218aa, Human, His-tag, E Coli (Bioactivity Validated)
Gene Names
HPRT1; HPRT; HGPRT
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
$230/0.02 mg | $405/0.1 mg | $165/0.004 mg | $1,570/5x0.1 mg
SDS-Page
TNFSF15, Active Protein (Cat# AAA204035)
Full Name
TNFSF15, 72-251aa, Human, His tag, E Coli
Gene Names
TNFSF15; TL1; TL1A; VEGI; TNLG1B; VEGI192A
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
$215/0.02 mg | $340/0.1 mg | $765/0.5 mg | $3,195/5x0.5 mg
Activity Data
(IL7 (Interleukin 7) is a hematopoietic growth factor secreted by stromal cells in the bone marrow and thymus. The interaction between IL17 and the IL7 receptor triggers a cascade of signals important for T-cell development within the thymus and survival within the periphery. It is reported that IL-7 acts on both resting and activated T cells, including Jurkat cells. Thus, a proliferation assay of recombinant human IL7 was conducted using Jurkat cells. Briefly, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL7. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Cell proliferation of Jurkat cells after incubation with IL7 for 72h observed by inverted microscope was shown in Figure 1. The CCK-8 data was shown in Figure 2. It was obvious that IL7 significantly promoted cell proliferation of Jurkat cells.)
Interleukin 7 (IL7), Active Protein (Cat# AAA2097240)
Full Name
Active Interleukin 7 (IL7)
Gene Names
IL7; IL-7
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$240/0.01 mg | $510/0.05 mg | $795/0.1 mg | $985/0.2 mg | $1,920/0.5 mg | $2,840/1 mg | $6,945/5 mg
Activity Data
(Figure. The binding activity of PAPPA with Plg.Pregnancy-associated plasma protein A (PAPPA), also known as pappalysin-1, is a secreted protease whose main substrate is insulin-like growth factor binding proteins. PAPPA's proteolytic function is activated upon collagen binding. It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses (fetuses with an abnormal number of chromosomes). Besides, Plasminogen (Plg) has been identified as an interactor of PAPPA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PAPPA and recombinant human Plg. Briefly, PAPPA were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to Plg-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PAPPA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PAPPA and Plg was shown in Figure 1, and this effect was in a dose dependent manner.)
Pregnancy Associated Plasma Protein A (PAPPA), Active Protein (Cat# AAA2105117)
Full Name
Active Pregnancy Associated Plasma Protein A (PAPPA)
Gene Names
PAPPA; PAPA; DIPLA1; PAPP-A; PAPPA1; ASBABP2; IGFBP-4ase
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$215/0.01 mg | $445/0.05 mg | $685/0.1 mg | $845/0.2 mg | $1,645/0.5 mg | $2,450/1 mg | $5,990/5 mg
Activity Data
(Figure. The binding activity of APOA1 with Hpt.Apolipoprotein A1 (APOA1) is the major protein component of HDL particles in plasma. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA1 was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. ApoA1 is often used as a biomarker for prediction of cardiovascular diseases. Besides, Haptoglobin (Hpt) has been identified as an interactor of APOA1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human APOA1 and recombinant human Hpt. Briefly, APOA1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to Hpt-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-APOA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of APOA1 and Hpt was shown in Figure 1, and this effect was in a dose dependent manner.)
Apolipoprotein A1 (APOA1), Active Protein (Cat# AAA2105095)
Full Name
Active Apolipoprotein A1 (APOA1)
Gene Names
APOA1; apo(a)
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$195/0.01 mg | $390/0.05 mg | $600/0.1 mg | $735/0.2 mg | $1,425/0.5 mg | $2,120/1 mg | $5,215/5 mg
Activity Data
(Deoxyribonuclease I (usually called DNase I) is a nonspecific endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. DNase I can be activated by bivalent metals such as Mg2 and Ca2 . This endonuclease enzyme is common reagents used in biochemical methods requiring diestion of DNA and recovery of RNA, or where DNA is to be removed without affecting structural proteins or enzymes. For example, DNase I is frequently used to remove template DNA following in vitro transcription, and to remove contaminating DNA in total RNA preparations (especially those from transfected cells that may contain plasmid DNA), used for ribonuclease protection assays, cDNA library contraction, and RT-PCR. Besides, Actin Beta (ACTb) has been identified as an interactor of DNase I, thus a binding ELISA assay was conducted to detect the interaction of recombinant human DNase I and recombinant human ACTb. Briefly, DNase I were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-DNase I pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of DNase I and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.)
Deoxyribonuclease I (DNASE1), Active Protein (Cat# AAA2097290)
Full Name
Active Deoxyribonuclease I (DNASE1)
Gene Names
DNASE1; DNL1; DRNI
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$175/0.01 mg | $335/0.05 mg | $510/0.1 mg | $625/0.2 mg | $1,205/0.5 mg | $1,790/1 mg | $4,385/5 mg
Activity Data
(The Interferon-alpha (IFNa) proteins belong to type I interferons (IFNs) which a large subgroup of interferon proteins that help regulate the activity of the immune system. The IFNa proteins produced by leukocytes are also known as leukocyte interferon. They are mainly involved in innate immune response against viral infection. Besides, Interferon-alpha 13 (IFNA13) has been identified as an interactor of IFNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IFNa and recombinant mouse IFNA13. Briefly, IFNA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IFNA13-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IFNapAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IFNa and IFNA13 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interferon Alpha (IFNa), Active Protein (Cat# AAA2097256)
Full Name
Active Interferon Alpha (IFNa)
Gene Names
Ifna1; Ifa1
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$255/0.01 mg | $565/0.05 mg | $875/0.1 mg | $1,080/0.2 mg | $2,120/0.5 mg | $3,145/1 mg | $7,695/5 mg
Activity Data
(Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. As reported, when the pheochromocytoma cell line PC12 is exposed to nerve growth factor (NGF), the cells respond over a period of a week by ceasing cell division and extending neurites (Greene and Tischler, 1976). The cells were grown in Ham's F12K containing 5% fetal calf serum and 10% horse serum on polylysine or collagen coated plates. When cells reached log phase growth , fresh medium was added together with 10ng/mL of NGF, then cells were observed by inverted microscope everyday.Cell division ceasing and differentiation of PC12 cells after incubation with NGF (10ng/mL) for 6 days was shown in Figure1.Control group which received no NGF displayed no neurite outgrowth and cells multiply rapidly.)
Nerve Growth Factor (NGF), Active Protein (Cat# AAA2086071)
Full Name
Active Nerve Growth Factor (NGF)
Gene Names
MMP8; HNC; CLG1; MMP-8; PMNL-CL
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$195/0.01 mg | $390/0.05 mg | $600/0.1 mg | $735/0.2 mg | $1,425/0.5 mg | $2,120/1 mg | $5,215/5 mg
SDS-Page
LGALS8, Active Protein (Cat# AAA204201)
Full Name
LGALS8, 1-316aa, mouse, His tag, E Coli (Bioactivity Validated)
Gene Names
Lgals8; Lgals-8; AI326142; D13Ertd524e; 1200015E08Rik
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
$215/0.01 mg | $340/0.05 mg | $765/0.25 mg | $3,195/5x0.25 mg
Activity Data
(IL11 (Interleukin-11) is a multifunctional cytokine first isolated from bone marrow-derived stromal cells. It stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Besides, IL11 is reported to induce the proliferation of human T-cells, thus, a proliferation assay was conducted to detect the bioactivity of human recombinant IL11 using Jurkat cells. Briefly, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL11. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Cell proliferation of Jurkat cells after incubation with IL11 for 72h observed by inverted microscope was shown in Figure 1. The CCK-8 data was shown in Figure 2. It was obvious that IL11 significantly promoted cell proliferation of Jurkat cells.)
Interleukin 11 (IL11), Active Protein (Cat# AAA2097260)
Full Name
Active Interleukin 11 (IL11)
Gene Names
IL11; AGIF; IL-11
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$255/0.01 mg | $565/0.05 mg | $875/0.1 mg | $1,080/0.2 mg | $2,120/0.5 mg | $3,145/1 mg | $7,695/5 mg
Activity Data
(Figure. The binding activity of MIF with MHCDG.Macrophage migration inhibitory factor (MIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein classified as an inflammatory cytokine. MIF is an important regulator of innate immunity. It involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. Besides, Major Histocompatibility Complex Class II Invariant Chain (MHCDG) has been identified as an interactor of MIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse MIF and recombinant mouse MHCDG. Briefly, MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCDG-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MIF and MHCDG was shown in Figure 1, and this effect was in a dose dependent manner.)
Macrophage Migration Inhibitory Factor (MIF), Active Protein (Cat# AAA2105113)
Full Name
Active Macrophage Migration Inhibitory Factor (MIF)
Gene Names
Mif; GIF; DER6; Glif
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$150/0.01 mg | $255/0.05 mg | $385/0.1 mg | $470/0.2 mg | $895/0.5 mg | $1,315/1 mg | $3,210/5 mg | $14,445/5x5 mg
Sequence
Galectin 3 (GAL3), Active Protein (Cat# AAA2105089)
Full Name
Active Galectin 3 (GAL3)
Gene Names
LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>98%
Pricing
$185/0.01 mg | $355/0.05 mg | $540/0.1 mg | $665/0.2 mg | $1,285/0.5 mg | $1,915/1 mg | $4,695/5 mg
Activity Data
(Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1,is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It can also affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. Besides, Colony Stimulating Factor Receptor, Macrophage (M-CFS-R) has been identified as an interactor of M-CSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse M-CSF and recombinant mouse MCFSR Briefly, M-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to M-CFS-R-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-M-CSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of M-CSF and M-CFS-R was shown in Figure 1, and this effect was in a dose dependent manner.)
Colony Stimulating Factor 1, Macrophage (MCSF), Active Protein (Cat# AAA2097263)
Full Name
Active Colony Stimulating Factor 1, Macrophage (MCSF)
Gene Names
Csf1; op; Csfm; MCSF; C87615
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$205/0.01 mg | $415/0.05 mg | $635/0.1 mg | $790/0.2 mg | $1,530/0.5 mg | $2,280/1 mg | $5,565/5 mg
Glutathione S-Transferase, Active Protein (Cat# AAA143838)
Full Name
Recombinant Glutathione S-Transferase His Tag
Purity
Greater than 95% as determined by SDS-PAGE.
Pricing
$180/0.01 mg | $250/0.05 mg | $2,380/1 mg | $10,490/5x1 mg
Sequence
Perforin 1 (PRF1), Active Protein (Cat# AAA2105135)
Full Name
Active Perforin 1 (PRF1)
Gene Names
PRF1; P1; PFP; HPLH2
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>95%
Pricing
$225/0.01 mg | $475/0.05 mg | $735/0.1 mg | $910/0.2 mg | $1,775/0.5 mg | $2,625/1 mg | $6,415/5 mg
Sequence
Lemur Tyrosine Kinase 3 (LMTK3), Active Protein (Cat# AAA2086088)
Full Name
Active Lemur Tyrosine Kinase 3 (LMTK3)
Gene Names
LMTK3; LMR3; TYKLM3; PPP1R101
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays; In vivo assays.
Purity
>95%
Pricing
$210/0.01 mg | $435/0.05 mg | $665/0.1 mg | $825/0.2 mg | $1,600/0.5 mg | $2,385/1 mg | $5,820/5 mg
Activity Data
(Vascular endothelial growth factor A (VEGF-A), a glycosylated mitogen, is known to be a vascular permeability factor and an endothelial cell growth factor secreted by the smooth muscle and endothelial cells. It has been reported that VEGF-A induces vascular permeability and growth, promotes monocyte/macrophage migration, and inhibits cell apoptosis and so on. To test the effect of VEGF-A on cell proliferation of ECV304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of VEGFA. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC .Cell proliferation of ECV304 cells after incubation with VEGFA for 72h observed by inverted microscope was shown in Figure 1.)
Vascular Endothelial Growth Factor A (VEGFA), Active Protein (Cat# AAA2086075)
Full Name
Active Vascular Endothelial Growth Factor A (VEGFA)
Gene Names
Vegfa; VPF; Vegf; VEGF-A; VEGF111; VEGF164
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$215/0.01 mg | $455/0.05 mg | $700/0.1 mg | $865/0.2 mg | $1,680/0.5 mg | $2,505/1 mg | $6,120/5 mg
Activity Data
(Figure. The binding activity of IGF1 with IGFBP3.Insulin-like growth factor 1 (IGF1), also called somatomedin C is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF1, mecasermin, is used for the treatment of growth failure. Besides, Insulin Like Growth Factor Binding Protein 3 (IGFBP3) has been identified as an interactor of IGF1, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IGF1 and recombinant mouse IGFBP3. Briefly, IGF1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to IGFBP3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IGF1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IGF1 and IGFBP3 was shown in Figure 1, and this effect was in a dose dependent manner.)
Insulin Like Growth Factor 1 (IGF1), Active Protein (Cat# AAA2105063)
Full Name
Active Insulin Like Growth Factor 1 (IGF1)
Gene Names
Igf1; Igf-1; Igf-I; C730016P09Rik
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$105/0.01 mg | $160/0.05 mg | $215/0.1 mg | $250/0.2 mg | $450/0.5 mg | $645/1 mg | $1,555/5 mg | $6,995/5x5 mg
Activity Data
(Protein S100B is a member of the S100 family. S100 proteins are EF-hand calcium-binding proteins and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Experimental results suggest that the receptor for advanced glycation end products (RAGE) plays important roles in mediating S100 protein-induced cellular signaling. Besides, rat RAGE shares similarities with human RAGE in amino acids sequence with the identity of 80.0%. Thus a binding ELISA assay was conducted to detect the interaction of recombinant rat S100B and recombinant human RAGE. Briefly, S100B were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100B mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of S100B and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.)
S100 Calcium Binding Protein B (S100B), Active Protein (Cat# AAA2097246)
Full Name
Active S100 Calcium Binding Protein B (S100B)
Gene Names
S100b; S100P
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$175/0.01 mg | $330/0.05 mg | $505/0.1 mg | $620/0.2 mg | $1,195/0.5 mg | $1,775/1 mg | $4,350/5 mg
