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Type
Reactivity
Gene Name
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Host
Application
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2533 results for " His Active" - showing 150-200
Activity Data
(Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) also known as mammary-derived growth inhibitor is a protein that in humans is encoded by the FABP3 gene. FABP3 is a small cytoplasmic protein (15kDa) released from cardiac myocytes following an ischemic episode. It is involved in active fatty acid metabolism where it transports fatty acids from the cell membrane to mitochondria for oxidation. Besides, Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) has been identified as an interactor of SOD1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FABP3 and recombinant human SOD1. Briefly, FABP3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to SOD1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FABP3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of FABP3 and SOD1 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of FABP3 with SOD1.)
Heart-type Fatty Acid Binding Protein (H-FABP), Active Protein (Cat# AAA2105133)
Full Name
Active Heart-type Fatty Acid Binding Protein (H-FABP)
Gene Names
FABP3; MDGI; FABP11; H-FABP; M-FABP; O-FABP
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$165/0.01 mg | $315/0.05 mg | $485/0.1 mg | $600/0.2 mg | $1,145/0.5 mg | $1,705/1 mg | $4,180/5 mg
Activity Data
(PTHrP (Parathyroid Hormone Related Protein ) is a neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. A binding ELISA assay was conducted to detect the association of PTHrP with PTHR1. Briefly, PTHrP were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL PTHrP were then transferred to PTHR1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PTHrP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PTHrP and PTHR1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Parathyroid Hormone Related Protein (PTHrP), Active Protein (Cat# AAA2097284)
Full Name
Active Parathyroid Hormone Related Protein (PTHrP)
Gene Names
Pthlh; PLP; Pthrp; PTH-like
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$225/0.01 mg | $475/0.05 mg | $735/0.1 mg | $910/0.2 mg | $1,775/0.5 mg | $2,625/1 mg | $6,415/5 mg
Testing Data
(Sequence)
Semaphorin 3A (SEMA3A), Active Protein (Cat# AAA2097322)
Full Name
Active Semaphorin 3A (SEMA3A)
Gene Names
SEMA3A; HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III
Applications
Cell culture, Activity Assays
Purity
>92
Pricing
$190/0.01 mg | $390/0.05 mg | $595/0.1 mg | $735/0.2 mg | $1,420/0.5 mg | $2,115/1 mg | $5,200/5 mg
SDS-Page
(Fig.SDS-PAGE analysis of Human Latent TGF-beta 1 protein, His Tag.)
Latent TGF-beta 1, Active Protein (Cat# AAA9718692)
Full Name
Human Latent TGF-beta 1 Protein, His Tag
Gene Names
TGFB1; CED; LAP; DPD1; TGFB; IBDIMDE; TGFbeta
Purity
>95% as determined by SDS-PAGE
Pricing
$460/0.01 mg | $715/0.02 mg | $1,210/0.05 mg | $5,425/5x0.05 mg
Interleukin-21, Active Protein (Cat# AAA143602)
Full Name
Recombinant Mouse Interleukin-21
Gene Names
Il21; IL-21
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
$250/0.01 mg | $4,845/1 mg | $180/0.002 mg | $21,590/5x1 mg
Activity Data
(Cytochrome P450 1A1 (CYP1A1) is a member of Cytochromes P450 superfamily of enzymes. Cytochromes P450 are a group of heme-thiolate monooxygenases. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH). Besides, Heat Shock 70kDa Protein 4 (HSPA4) has been identified as an interactor of CYP1A1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CYP1A1 and recombinant rat HSPA4. Briefly, CYP1A1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSPA4-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CYP1A1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of CYP1A1 and HSPA4 was shown in Figure 1, and this effect was in a dose dependent manner.)
Cytochrome P450 1A1 (CYP1A1), Active Protein (Cat# AAA2097311)
Full Name
Active Cytochrome P450 1A1 (CYP1A1)
Gene Names
Cyp1a1; AHH; AHRR; CP11; CYP1; Cyp45c; P1-450; P450-C; P450DX; Cypc45c; P-450MC
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$255/0.01 mg | $565/0.05 mg | $875/0.1 mg | $1,080/0.2 mg | $2,120/0.5 mg | $3,145/1 mg | $7,695/5 mg
Activity Data
(Figure. The chemotactic effect of NAP3 on THP-1 cells.Neutrophil-activating protein 3 (NAP3) also known as chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family. NAP3 is expressed by macrophages, neutrophils and epithelial cells,and has neutrophil chemoattractant activity. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of NAP3 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension, 106 cells/mL in RPMI 1640 with FBS free) and SLC (31.25ng/mL, 62.5ng/mL,125ng/mL and 250ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37 degree C with 5% CO2 for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows NAP3 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical results were shown in Figure 2. The optimum chemotaxis of NAP3 occurs at 31.25-250ng/mL.(A) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 with 62.5ng/mL NAP3 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h; (B) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 without NAP3 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h.)
Neutrophil Activating Protein 3 (NAP3), Active Protein (Cat# AAA2105058)
Full Name
Active Neutrophil Activating Protein 3 (NAP3)
Gene Names
CXCL1; FSP; GRO1; GROa; MGSA; NAP-3; SCYB1; MGSA-a
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$145/0.01 mg | $255/0.05 mg | $375/0.1 mg | $460/0.2 mg | $870/0.5 mg | $1,280/1 mg | $3,125/5 mg | $14,055/5x5 mg
Sequence
Asporin (ASPN), Active Protein (Cat# AAA2105172)
Full Name
Active Asporin (ASPN)
Gene Names
Aspn; Plap1; Slrr1c; AA986886; 4631401G09Rik
Reactivity
Mus musculus (Mouse)
Applications
Cell culture; Activity Assays.
Purity
>95%
Pricing
$210/0.01 mg | $435/0.05 mg | $665/0.1 mg | $825/0.2 mg | $1,600/0.5 mg | $2,385/1 mg | $5,820/5 mg
Activity Data
(CCL13 (C-C motif chemokine 13) is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, which belongs to the CC chemokine subfamily. It has been reported that CCL13 can induce chemotactic migration of THP-1 cells. Therefore, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of CCL13 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension, 106 cells/mL in RPMI 1640 with 0.5% FBS) and CCL13 (50ng/mL and 100ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37oC with 5% CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).Result: CCL13 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen to count the migrated cells at high magnification (×400) and the statistical data was shown in Figure 2.)
Monocyte Chemotactic Protein 4 (MCP4), Active Protein (Cat# AAA2086077)
Full Name
Active Monocyte Chemotactic Protein 4 (MCP4)
Gene Names
CCL13; NCC1; CKb10; MCP-4; NCC-1; SCYL1; SCYA13
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$145/0.01 mg | $250/0.05 mg | $365/0.1 mg | $445/0.2 mg | $845/0.5 mg | $1,245/1 mg | $3,040/5 mg | $13,670/5x5 mg
Sequence Information
Laminin Gamma 1 (LAMC1), Recombinant Protein (Cat# AAA2018823)
Full Name
Recombinant Laminin Gamma 1 (LAMC1)
Gene Names
LAMC1; LAMB2
Reactivity
Human
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
> 97%
Pricing
$155/0.01 mg | $280/0.05 mg | $430/0.1 mg | $525/0.2 mg | $1,005/0.5 mg | $1,480/1 mg | $3,625/5 mg | $16,310/5x5 mg
Activity Data
(CYP1A2 (Cytochrome P450 1A2) belongs to the group of proteins which contains heme as a cofactor. CYP1A2 oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Besides, ASAH1 (Acid ceramidase) has been identified as an interactor of CYP1A2 through affinity capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1A2 and recombinant human ASAH1. Briefly, CYP1A2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ASAH1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CYP1A2 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CYP1A2 and ASAH1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Cytochrome P450 1A2 (CYP1A2), Active Protein (Cat# AAA2097310)
Full Name
Active Cytochrome P450 1A2 (CYP1A2)
Gene Names
CYP1A2; CP12; CYPIA2; P3-450; P450(PA)
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$265/0.01 mg | $595/0.05 mg | $920/0.1 mg | $1,135/0.2 mg | $2,240/0.5 mg | $3,315/1 mg | $8,120/5 mg
Activity Data
(Cold-inducible RNA-binding protein (CIRBP) plays a critical role in controlling the cellular response upon confronting a variety of cellular stresses, including short wavelength ultraviolet light, hypoxia, and hypothermia. Besides, Heterogeneous Nuclear Ribonucleoprotein A1 (HNRPA1) has been identified as an interactor of CIRBP, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse CIRBP and recombinant mouse HNRPA1. Briefly, CIRBP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HNRPA1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CIRBP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CIRBP and HNRPA1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Cold Inducible RNA Binding Protein (CIRBP), Active Protein (Cat# AAA2105188)
Full Name
Active Cold Inducible RNA Binding Protein (CIRBP)
Reactivity
Mus musculus (Mouse)
Applications
Cell culture; Activity Assays.
Purity
>95%
Pricing
$210/0.01 mg | $430/0.05 mg | $660/0.1 mg | $815/0.2 mg | $1,585/0.5 mg | $2,360/1 mg | $5,770/5 mg
Activity Data
(Figure. The binding activity of TGM1 with SNCa.Transglutaminase 1(TGM1) is an enzyme found in cells that make up the outermost layer of the skin (the epidermis). It can catalyze the addition of an alkyl group from an akylamine to a glutamine residue of a protein, forming an alkylglutamine in the protein. TGM1 is involved in the formation of the cornified cell envelope, which is a structure that surrounds skin cells and helps form a protective barrier between the body and its environment. Besides, Synuclein Alpha (SNCa) has been identified as an interactor of TGM1, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse TGM1 and recombinant mouse SNCa. Briefly, TGM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SNCa-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TGM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TGM1 and SNCa was shown in Figure 1, and this effect was in a dose dependent manner.)
Transglutaminase 1, Keratinocyte (TGM1), Active Protein (Cat# AAA2105150)
Full Name
Active Transglutaminase 1, Keratinocyte (TGM1)
Gene Names
Tgm1; Tgase1; 2310004J08Rik
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$230/0.01 mg | $505/0.05 mg | $785/0.1 mg | $970/0.2 mg | $1,895/0.5 mg | $2,805/1 mg | $6,860/5 mg
Activity Data
(INS (Insulin) is a peptide hormone produced by beta cells of the pancreatic islets, which decreases blood glucose concentration and increases cell permeability to monosaccharides, amino acids and fatty acids. It has been reported that insulin triggers phosphorylation of a number of substrates by binding to its receptors, which was important for cell proliferation, cell cycle progression, cell division and differentiation. To detect the effect of Insulin on cell proliferation, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of INS. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C.)
Insulin (INS), Active Protein (Cat# AAA2086079)
Full Name
Active Insulin (INS)
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$175/0.01 mg | $330/0.05 mg | $505/0.1 mg | $620/0.2 mg | $1,195/0.5 mg | $1,775/1 mg | $4,350/5 mg
Activity Data
(Figure. Cell proliferation of TF-1 cells after stimulated with LIF.Leukemia Inhibitory Factor (LIF), is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. p53 regulated LIF has been shown to facilitate implantation in the mouse model and possibly in humans. To test the effect of LIF on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 including various concentrations of recombinant human LIF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of TF-1 cells after incubation with LIF for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant LIF for 72h. The result was shown in Figure 2. It was obvious that LIF significantly increased cell viability of TF-1 cells.(A) TF-1 cells cultured in 1640, stimulated with 0.5ng/mL LIF for 72h; (B) Unstimulated TF-1 cells cultured in 1640 for 72h.)
Leukemia Inhibitory Factor (LIF), Active Protein (Cat# AAA2097262)
Full Name
Active Leukemia Inhibitory Factor (LIF)
Gene Names
LIF; CDF; DIA; HILDA; MLPLI
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$165/0.01 mg | $310/0.05 mg | $480/0.1 mg | $585/0.2 mg | $1,120/0.5 mg | $1,660/1 mg | $4,075/5 mg
SDS-Page
ACP5, Active Protein (Cat# AAA206334)
Full Name
ACP5, 22-325aa, Human, His tag, Baculovirus (Bioactivity Validated)
Gene Names
ACP5; HPAP; TRAP; TRACP5a; TRACP5b; TrATPase
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
$215/0.01 mg | $340/0.05 mg | $985/0.25 mg | $4,180/5x0.25 mg
Testing Data
(Sequence)
Fibronectin (FN), Active Protein (Cat# AAA2097257)
Full Name
Active Fibronectin (FN)
Gene Names
FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; SMDCF
Applications
Cell culture, Activity Assays
Purity
>98%
Pricing
$175/0.01 mg | $335/0.05 mg | $510/0.1 mg | $625/0.2 mg | $1,205/0.5 mg | $1,790/1 mg | $4,385/5 mg
Sequence
Glutathione S Transferase Pi (GSTp), Active Protein (Cat# AAA2097250)
Full Name
Active Glutathione S Transferase Pi (GSTp)
Applications
Cell culture; Activity Assays; In vivo assays
Purity
>95%
Pricing
$185/0.01 mg | $360/0.05 mg | $550/0.1 mg | $675/0.2 mg | $1,310/0.5 mg | $1,950/1 mg | $4,785/5 mg
Activity Data
(NOG (Noggin) is a signaling molecule that plays an important role in promoting somite patterning in the developing embryo, essential for cartilage morphogenesis and joint formation. It is considered as an inhibitor in bone morphogenetic proteins (BMP) signaling, by binding with BMP4, thus a binding ELISA assay was conducted to detect the association of recombinant rat NOG with BMP4. Briefly, NOG were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP4-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-NOG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of NOG with BMP4 was shown in Figure 1 and this effect was in a dose dependent manner.)
Noggin (NOG), Active Protein (Cat# AAA2097352)
Full Name
Active Noggin (NOG)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$205/0.01 mg | $420/0.05 mg | $645/0.1 mg | $800/0.2 mg | $1,550/0.5 mg | $2,310/1 mg | $5,640/5 mg
Sequence Information
Interleukin 10 Receptor Beta (IL10Rb), Active Protein (Cat# AAA2097221)
Full Name
Active Interleukin 10 Receptor Beta (IL10Rb)
Gene Names
IL10RB; CRFB4; CRF2-4; D21S58; D21S66; CDW210B; IL-10R2
Reactivity
Homo sapiens (Human)
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
>98%
Pricing
$180/0.01 mg | $350/0.05 mg | $535/0.1 mg | $655/0.2 mg | $1,265/0.5 mg | $1,880/1 mg | $4,610/5 mg
Activity Data
(Figure. The binding activity of ODC with TK1.Ornithine decarboxylase (ODC) is an enzyme can catalyze the decarboxylation of ornithine (a product of the urea cycle) to form putrescine. The ornithine decarboxylation reaction catalyzed by ornithine decarboxylase is the first and committed step in the synthesis of polyamines, particularly putrescine, spermidine and spermine. Polyamines are important for stabilizing DNA structure, the DNA double strand-break repair pathway and as antioxidants. Therefore, ornithine decarboxylase is an essential enzyme for cell growth, producing the polyamines necessary to stabilize newly synthesized DNA. Lack of ODC causes cell apoptosis in embryonic mice, induced by DNA damage. Besides, Thymidine Kinase 1, Soluble (TK1) has been identified as an interactor of ODC, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ODC and recombinant human TK1. Briefly, ODC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to TK1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ODC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ODC and TK1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Ornithine Decarboxylase (ODC), Active Protein (Cat# AAA2105137)
Full Name
Active Ornithine Decarboxylase (ODC)
Gene Names
ODC1; ODC
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$225/0.01 mg | $490/0.05 mg | $755/0.1 mg | $940/0.2 mg | $1,830/0.5 mg | $2,710/1 mg | $6,620/5 mg
Activity Data
(IL1R1 (Interleukin 1 Receptor Type I), also known as CD121a, is an important mediator involved in many cytokine induced immune and inflammatory responses. It belongs to the interleukin-1 receptor family, and is a receptor for interleukin 1 alpha (IL1a), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). Thus, a binding ELISA assay was conducted to detect the association of IL1R1 with IL1a. Briefly, IL1R1 were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1a-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL1R1 with IL1a was shown in Figure 1 and this effect was in a dose dependent manner.)
Interleukin 1 Receptor Type I (IL1R1), Active Protein (Cat# AAA2097233)
Full Name
Active Interleukin 1 Receptor Type I (IL1R1)
Gene Names
IL1R1; P80; IL1R; IL1RA; CD121A; D2S1473; IL-1R-alpha
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$160/0.01 mg | $300/0.05 mg | $460/0.1 mg | $565/0.2 mg | $1,080/0.5 mg | $1,600/1 mg | $3,920/5 mg
Sequence
Peptidylglycine Alpha Amidating Monooxygenase (PAM), Active Protein (Cat# AAA2105174)
Full Name
Active Peptidylglycine Alpha Amidating Monooxygenase (PAM)
Gene Names
Pam; PHM
Reactivity
Rattus norvegicus (Rat)
Applications
Cell culture; Activity Assays
Purity
>96%
Pricing
$270/0.01 mg | $610/0.05 mg | $945/0.1 mg | $1,165/0.2 mg | $2,295/0.5 mg | $3,400/1 mg | $8,330/5 mg
Activity Data
(Vitamin D Receptor (VDR) is the nuclear hormone receptor for vitamin D3. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. It mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Besides, E1A binding protein p300 (EP300) has been identified as an interactor of VDR, thus a binding ELISA assay was conducted to detect the interaction of recombinant human VDR and recombinant human EP300. Briefly, VDR were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EP300-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-VDR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of VDR and EP300 was shown in Figure 1, and this effect was in a dose dependent manner.)
Vitamin D Receptor (VDR), Active Protein (Cat# AAA2097274)
Full Name
Active Vitamin D Receptor (VDR)
Gene Names
VDR; NR1I1; PPP1R163
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$230/0.01 mg | $495/0.05 mg | $765/0.1 mg | $945/0.2 mg | $1,845/0.5 mg | $2,730/1 mg | $6,670/5 mg
Activity Data
(Figure. The binding activity of CAPN3 with TTN.Calpain 3 is a calcium-dependent cysteine protease mainly expressed in skeletal muscle. In humans, calpain 3 is encoded by the CAPN3 gene. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. Besides, Titin (TTN) has been identified as an interactor of CAPN3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CAPN3 and recombinant human TTN. Briefly, CAPN3 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TTN-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CAPN3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CAPN3 and TTN was shown in Figure 1, and this effect was in a dose dependent manner.)
Calpain 3 (CAPN3), Active Protein (Cat# AAA2105179)
Full Name
Active Calpain 3 (CAPN3)
Gene Names
CAPN3; p94; CANP3; LGMD2; nCL-1; CANPL3; LGMD2A; LGMDD4; LGMDR1
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$225/0.01 mg | $485/0.05 mg | $755/0.1 mg | $935/0.2 mg | $1,820/0.5 mg | $2,695/1 mg | $6,585/5 mg
Interleukin-3, Active Protein (Cat# AAA142358)
Full Name
Recombinant Human Interleukin-3, His Tag
Gene Names
IL3; IL-3; MCGF; MULTI-CSF
Purity
Greater than 90.0% as determined by analysis by SDS-PAGE.
Pricing
$180/0.01 mg | $250/0.05 mg | $2,905/1 mg | $12,845/5x1 mg
Sequence
Endothelial Cell Specific Molecule 1 (ESM1), Active Protein (Cat# AAA2097304)
Full Name
Active Endothelial Cell Specific Molecule 1 (ESM1)
Gene Names
ESM1; endocan
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>92%
Pricing
$225/0.01 mg | $490/0.05 mg | $760/0.1 mg | $940/0.2 mg | $1,830/0.5 mg | $2,710/1 mg | $6,630/5 mg
Activity Data
(Figure. Cell proliferation of Raw264.7 cells after stimulated with SCF.Stem cell factor (also known as SCF, KIT-ligand, KL, or steel factor) is a cytokine that binds to the c-KIT receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis. SCF has been shown to stimulate the proliferation of Raw264.7 cells. To test this effect, Raw264.7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach later, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM containing various concentrations of recombinant mouse SCF. After incubated for 120h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of Raw264.7 cells after incubation with SCF for 120h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant SCF for 120h. The result was shown in Figure 2. It was obvious that SCF significantly increased cell viability of Raw264.7 cells.(A) Raw264.7 cells cultured in DMEM, stimulated with 1ng/mL SCF for 120h; (B) Unstimulated Raw264.7 cells cultured in DMEM for 120h.)
Stem Cell Factor (SCF), Active Protein (Cat# AAA2105078)
Full Name
Active Stem Cell Factor (SCF)
Gene Names
Kitl; Gb; SF; Sl; Clo; Con; Mgf; SCF; SLF; blz; Kitlg; contrasted
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$230/0.01 mg | $500/0.05 mg | $770/0.1 mg | $955/0.2 mg | $1,860/0.5 mg | $2,755/1 mg | $6,740/5 mg
Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL), Active Protein (Cat# AAA2097237)
Full Name
Active Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)
Gene Names
TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L; TNLG6A
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays; In vivo assays.
Purity
>95%
Pricing
$150/0.01 mg | $270/0.05 mg | $400/0.1 mg | $485/0.2 mg | $935/0.5 mg | $1,375/1 mg | $3,360/5 mg | $15,105/5x5 mg
Activity Data
(Interleukin-2 (IL-2), the main lymphokine produced by activated CD4 T-helper lymphocytes, is a potent T-cell growth regulator. In addition to this activity, IL-2 was found to decrease the proliferation of 3T3 fibroblasts in time- and dose-dependent manner. Thus 3T3 cells were seeded overnight at a density of 5,000 cells/well, and treated with or without various concentrations of IL2 for 48h, then 3T3 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC.The inhibitory effect of IL-2 on cell proliferation of 3T3 cells observed by inverted microscope was shown in Figure 1.)
Interleukin 2 (IL2), Active Protein (Cat# AAA2086063)
Full Name
Active Interleukin 2 (IL2)
Gene Names
Il2; Il-2
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$150/0.01 mg | $255/0.05 mg | $385/0.1 mg | $470/0.2 mg | $895/0.5 mg | $1,315/1 mg | $3,210/5 mg | $14,445/5x5 mg
Activity Data
(Figure. The binding activity of VEGF165 with VEGFR1.Vascular endothelial growth factor 165 is one kind of isoforms of Vascular endothelial growth factor A (VEGFA). This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Besides, Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) has been identified as an interactor of VEGF165, thus a binding ELISA assay was conducted to detect the interaction of recombinant human VEGF165 and recombinant human VEGFR1. Briefly, VEGF165 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to VEGFR1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-VEGF165 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of VEGF165 and VEGFR1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Vascular Endothelial Growth Factor 165 (VEGF165), Active Protein (Cat# AAA2105148)
Full Name
Active Vascular Endothelial Growth Factor 165 (VEGF165)
Gene Names
VEGFA; VPF; VEGF; MVCD1
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$240/0.01 mg | $515/0.05 mg | $800/0.1 mg | $990/0.2 mg | $1,935/0.5 mg | $2,865/1 mg | $7,005/5 mg
Sequence Information
Platelet Derived Growth Factor Receptor Beta, Recombinant Protein (Cat# AAA2030725)
Full Name
Recombinant Platelet Derived Growth Factor Receptor Beta (PDGFRb)
Gene Names
Pdgfrb; PDGFR-1
Reactivity
Mus musculus (Mouse)
Applications
WB
Purity
> 97%
Pricing
$160/0.01 mg | $285/0.05 mg | $440/0.1 mg | $540/0.2 mg | $1,035/0.5 mg | $1,525/1 mg | $3,730/5 mg
Activity Data
(SDF1 (stromal cell-derived factor 1), also known as C-X-C motif chemokine 12, is a chemokine protein that has chemotaxis active on T-lymphocytes and monocytes. It is thought that SDF1 stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3; thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of SDF1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension,106cells/mL in RPMI 1640 with 0.5% FBS) and SDF1 (10ng/mL, 30ng/mL and 60ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37 degree C with 5% CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows SDF1 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical results were shown in Figure 2. The optimum chemotaxis of SDF1 occurs at 10ng/mL.)
Stromal Cell Derived Factor 1 (SDF1), Active Protein (Cat# AAA2097266)
Full Name
Active Stromal Cell Derived Factor 1 (SDF1)
Gene Names
CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$225/0.01 mg | $475/0.05 mg | $735/0.1 mg | $910/0.2 mg | $1,775/0.5 mg | $2,625/1 mg | $6,415/5 mg
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Cathepsin X/Z/P, Active Protein (Cat# AAA201142)
Full Name
Recombinant Cathepsin X/Z/P
Gene Names
Ctsz; CTSX; AI787083; AU019819; D2Wsu143e
Applications
SDS-PAGE, Enzyme Activity
Purity
>95% by SDS-PAGE
Pricing
$300/0.01 mg | $650/0.05 mg | $2,675/5x0.05 mg
Activity Data
(Measured in a cell proliferation assay using mouse BMDC (bone marrow derived dendritic cells). The ED50 (median effective dose) for this effect is less than 0.25 ng/mL.In-house data of APA045Mu01 used in cellular experiment:Six-eight weeks old Balb/c mice were used for BMDC. At first, mouse femur and tibia were taken out, and then bone marrow was washed out with serum-free RPMI 1640 medium, followed by centrifugation at 1200RPM for 5min (4oC). ACK buffer was added to get rid of red blood cells, and then, centrifuged at 1200RPM for 5min (4oC). Cell pellets were collected and re-suspended, the cells were cultured in DMEM medium supplemented with 10% FBS, or DMEM medium supplemented with 10% FBS and GMCSF (APA045Mu01) at 37oC with 5% CO2 in thermostatic incubator. Three days later, cells were observed by microscope, and the result is shown in Figure 1.)
Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF), Active Protein (Cat# AAA2086056)
Full Name
Active Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)
Gene Names
Csf2; Csfgm; GMCSF; Gm-CSf; MGI-IGM
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$210/0.01 mg | $435/0.05 mg | $670/0.1 mg | $830/0.2 mg | $1,610/0.5 mg | $2,400/1 mg | $5,865/5 mg
Activity Data
(Insulin (INS) is a polypeptide hormone originating in the beta cells of the pancreas and serving as a principal regulator for the storage and production of carbohydrates. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Besides, Insulin Receptor (ISR) has been identified as an interactor of INS, thus a binding ELISA assay was conducted to detect the interaction of recombinant bovine INS and recombinant human ISR. Briefly, INS were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ISR-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-INS pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of INS and ISR was shown in Figure 1, and this effect was in a dose dependent manner.)
Insulin (INS), Active Protein (Cat# AAA2097273)
Full Name
Active Insulin (INS)
Reactivity
Bovine
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
Elcatonin, Active Protein (Cat# AAA143228)
Full Name
Elcatonin
Purity
Greater than 93.3% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
$180/0.2 mg | $250/1 mg | $2,830/100 mg | $12,520/5x100 mg
Activity Data
(Figure 1. The binding activity of CKB with CKM.Creatine Kinase, Brain (CKB) also known as CK-BB is a creatine kinase. CKB, consists of a homodimer of two identical brain-type CK-B subunits, is a cytoplasmic enzyme involved in cellular energy homeostasis, with certain fractions of the enzyme being bound to cell membranes, ATPases, and a variety of ATP-requiring enzymes in the cell. Besides, Creatine Kinase, Muscle (CKM) has been identified as an interactor of CKB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CKB and recombinant human CKM. Briefly, CKB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CKM-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CKB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CKB and CKM was shown in Figure 1, and this effect was in a dose dependent manner.)
Creatine Kinase, Brain (CKB), Active Protein (Cat# AAA2105161)
Full Name
Active Creatine Kinase, Brain (CKB)
Gene Names
CKB; BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$225/0.01 mg | $480/0.05 mg | $735/0.1 mg | $910/0.2 mg | $1,770/0.5 mg | $2,640/1 mg | $6,455/5 mg
Activity Data
(Arginase (Arg) is an enzyme that catalyzes the degradation of arginine to produce urea and omithine, which is crucial in the urea cycle. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. Besides, Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5) has been identified as an interactor of Arg, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse Arg and recombinant mouse UCHL5. Briefly, Arg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCHL5-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-Arg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of Arg and UCHL5 was shown in Figure 1, and this effect was in a dose dependent manner.)
Arginase (ARG), Active Protein (Cat# AAA2097288)
Full Name
Active Arginase (ARG)
Gene Names
Arg1; AI; PGIF; Arg-1; AI256583
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$225/0.01 mg | $485/0.05 mg | $750/0.1 mg | $930/0.2 mg | $1,815/0.5 mg | $2,685/1 mg | $6,570/5 mg
Activity Data
(IL36G (Interleukin 36 gamma), also known as IL-1F9 and IL-1H1 is a member of the IL-1 family. The receptor for IL36 gamma is reported to be a combination of IL-1 R6/IL-1 R rp2 and IL-1 R3/IL-1 R AcP. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant human IL36G with recombinant human IL1R1. Briefly, IL36G were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1R1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL36G with IL1R1 was shown in Figure 1 and this effect was in a dose dependent manner.)
Interleukin 1 Family, Member 9 (IL1F9), Active Protein (Cat# AAA2097326)
Full Name
Active Interleukin 1 Family, Member 9 (IL1F9)
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$245/0.01 mg | $540/0.05 mg | $840/0.1 mg | $1,040/0.2 mg | $2,030/0.5 mg | $3,005/1 mg | $7,350/5 mg
Activity Data
(Fibronectin (FN) is a high-molecular weight (~440kDa) glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectin has numerous functions. For example, it involved in cell adhesion, cell motility, opsonization, wound healing, maintenance of cell shape, and so on. Besides, Connective Tissue Growth Factor (CTGF) has been identified as an interactor of FN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FN and recombinant human CTGF. Briefly, FN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CTGF-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of FN and CTGF was shown in Figure 1, and this effect was in a dose dependent manner.)
Fibronectin (FN), Active Protein (Cat# AAA2097258)
Full Name
Active Fibronectin (FN)
Gene Names
FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; SMDCF
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$185/0.01 mg | $365/0.05 mg | $560/0.1 mg | $690/0.2 mg | $1,335/0.5 mg | $1,985/1 mg | $4,870/5 mg
Sequencing
Paraoxonase 1 (PON1), Active Protein (Cat# AAA2090457)
Full Name
Active Paraoxonase 1 (PON1)
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>98%
Pricing
$255/0.01 mg | $575/0.05 mg | $895/0.1 mg | $1,100/0.2 mg | $2,170/0.5 mg | $3,210/1 mg | $7,865/5 mg
SDS-Page
(3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
NMNAT1, Active Protein (Cat# AAA206313)
Full Name
NMNAT1, 115-270aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
NMNAT1; LCA9; NMNAT; PNAT1
Applications
SDS-PAGE, Enzyme Activity
Purity
> 95% by SDS-PAGE
Pricing
$190/0.01 mg | $230/0.02 mg | $290/0.05 mg | $405/0.1 mg | $985/0.5 mg | $630/0.25 mg | $4,180/5x0.5 mg
SDS-Page
Hexokinase 2, Active Protein (Cat# AAA203048)
Full Name
Hexokinase 2,1-917 aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
HK2; HKII; HXK2
Applications
SDS-PAGE
Purity
> 85% by SDS-PAGE
Pricing
$275/0.02 mg | $550/0.1 mg | $1,410/0.5 mg | $6,110/5x0.5 mg
Activity Data
(Platelet Derived Growth Factor Subunit A (PDGFA) is a member of Platelet-derived growth factor (PDGF) family which plays a significant role in blood vessel formation, the growth of blood vessels from already-existing blood vessel tissue, mitogenesis, i.e. proliferation, of mesenchymal cells such as fibroblasts, osteoblasts, tenocytes, vascular smooth muscle cells and mesenchymal stem cells as well as chemotaxis, the directed migration, of mesenchymal cells. Besides, Platelet Derived Growth Factor Receptor Alpha (PDGFRa) has been identified as an interactor of PDGFA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PDGFA and recombinant human PDGFRa. Briefly, PDGFA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100mul were then transferred to PDGFRa-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PDGFA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PDGFA and PDGFRa was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of PDGFA with PDGFRa.)
Platelet Derived Growth Factor Subunit A (PDGFA), Active Protein (Cat# AAA2105097)
Full Name
Active Platelet Derived Growth Factor Subunit A (PDGFA)
Gene Names
PDGFA; PDGF1; PDGF-A
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$225/0.01 mg | $475/0.05 mg | $735/0.1 mg | $910/0.2 mg | $1,775/0.5 mg | $2,625/1 mg | $6,415/5 mg
Sequence
Osteopontin (OPN), Active Protein (Cat# AAA2086084)
Full Name
Active Osteopontin (OPN)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays; In vivo assays.
Purity
>95%
Pricing
$120/0.01 mg | $190/0.05 mg | $265/0.1 mg | $310/0.2 mg | $590/0.5 mg | $860/1 mg | $2,085/5 mg | $9,385/5x5 mg
Sequence
Phospholipase A2, Lipoprotein Associated (LpPLA2), Active Protein (Cat# AAA2105125)
Full Name
Active Phospholipase A2, Lipoprotein Associated (LpPLA2)
Gene Names
PLA2G7; PAFAD; PAFAH; LP-PLA2; LDL-PLA2
Reactivity
Homo sapiens (Human)
Applications
Cell Culture, Activity Assays
Purity
>95%
Pricing
$210/0.01 mg | $425/0.05 mg | $650/0.1 mg | $805/0.2 mg | $1,565/0.5 mg | $2,330/1 mg | $5,690/5 mg
Activity Data
(Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. NGF is also involved in pathways besides those regulating the life cycle of neurons. a nerve growth factor (NGF)-dependent TF-1 subclone cell line suitable for determination of bioactivity of recombinant NGF(rhNGF), then TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 contain various concentrations of recombinant rat NGF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of TF-1 cells after incubation with TF-1 for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 assay after incubation with recombinant NGF for 72h. The result was shown in Figure 2. It was obvious that NGF significantly increased cell viability of TF-1 cells.(A) TF-1 cells cultured in 1640, stimulated with 0.1ng/mL NGF for 72h; (B) Unstimulated TF-1 cells cultured in 1640 for 72h.Figure. Cell proliferation of TF-1 cells after stimulated with NGF.)
Nerve Growth Factor (NGF), Active Protein (Cat# AAA2105076)
Full Name
Active Nerve Growth Factor (NGF)
Gene Names
Ngf; Ngfb; beta-NGF
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$150/0.01 mg | $255/0.05 mg | $385/0.1 mg | $470/0.2 mg | $895/0.5 mg | $1,315/1 mg | $3,210/5 mg | $14,445/5x5 mg
Sequence Information
Apolipoprotein C3, Recombinant Protein (Cat# AAA2030861)
Full Name
Recombinant Apolipoprotein C3 (APOC3)
Gene Names
APOC3; HALP2; APOCIII
Reactivity
Human
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
>92%
Pricing
$145/0.01 mg | $255/0.05 mg | $375/0.1 mg | $460/0.2 mg | $875/0.5 mg | $1,285/1 mg | $3,145/5 mg | $14,135/5x5 mg
Activity Data
(Regenerating Islet Derived Protein 3 Beta (REG3b) also known as PAP-I and HIP is bactericidal C-type lectin which acts against several intestinal Gram-positive bacteria and Gram-negative bacteria. The Reg family proteins have been implicated in a range of physiological processes including acting as acute phase reactants, lectins, survival/growth factors for insulin-producing pancreatic beta-cells, neural cells, and epithelial cells of the digestive system. To test the effect of REG3b on cell proliferation of SK-N-SH, SK-N-SH cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of REG3b. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C .Cell proliferation of SK-N-SH cells after incubation with REG3b for 72h observed by inverted microscope was shown in Figure 1.)
Regenerating Islet Derived Protein 3 Beta (REG3b), Active Protein (Cat# AAA2097321)
Full Name
Active Regenerating Islet Derived Protein 3 Beta (REG3b)
Gene Names
Reg3b; HIP; Pap; PAP1; REG-III
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$235/0.01 mg | $510/0.05 mg | $790/0.1 mg | $980/0.2 mg | $1,915/0.5 mg | $2,830/1 mg | $6,925/5 mg
