GST active protein

GST, 1-218aa, Schistosoma japonicum, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA203606

• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: GST; 1-218aa; Schistosoma japonicum; His tag; E Coli (Bioactivity Validated); Glutathione S-Transferase class-mu 26 kDa isozyme; Sj26 antigen; SjGST; Glutathione S-Transferase class-mu 26 kDa isozyme Glutathione S Transferase.; GST active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4), 10% glycerol
• Concentration: 1 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR
• Sequence Length: 218

• Applicable Applications for GST active protein: SDS-PAGE
• Antigen Species: Schistosoma japonicum
• Tag Information: His-tag
• Biological Activity: Specific activity is > 10 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
• Assay: 1. Prepare a 180ul assay buffer into a suitable container. The concentrations are 106mM potassium phosphate (pH6.5), 1.1mM EDTA, 5.5mM glutathione, reduced, 2.2mM 1-chloro-2,4-dinitrobenzene (CDNB), 8% (v/v) ethanol.; 2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer.; 3. Add 20ul of recombinant GST protein 12.5ug/ml in assay buffer.; 4. Mix by inversion and record the increase at A340nm for approximately 5 minutes.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GST active protein

Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E Coli source. Recombinant Schistosoma japonicum GST, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for GST active protein

Enzymes & Proteases

References

Hayes JD., et al. (1995). Crit Rev Biochem Mol Biol. 30 (6):445-600; Bekett GJ., et al. (1987). J. Clin Biochem Nutr. 2:1-24; Smith DB., et al. (1988). Mol Biochem Parasitol. 27 (2-3):249-56.;

NCBI and Uniprot Product Information

• NCBI GI #: 160927
• Molecular Weight: 28.3 kDa (244aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: Sj26 antigen
• UniProt Protein Name: Glutathione S-transferase class-mu 26 kDa isozyme
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GST 26
• UniProt Synonym Gene Names: GST 26

Uniprot Description

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.