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Activity Data
(Lemur tyrosine kinase 3 (LMTK3), a member of the receptor tyrosine kinase (RTK) family, is implicated in breast cancer growth and endocrine resistance. It has been reported that LMTK3 promotes cell invasion, motility, and migration of the MCF-7 breast cancer cell line. To test the bioactivity of LMTK3, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of LMTK3. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C.Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown in Figure 1.)
Lemur Tyrosine Kinase 3 (LMTK3) Active Protein | LMTK3 active protein
Active Lemur Tyrosine Kinase 3 (LMTK3)
Gene Names
LMTK3; LMR3; TYKLM3; PPP1R101
Applications
Cell culture, Activity Assays
Purity
> 95%
Synonyms
Lemur Tyrosine Kinase 3 (LMTK3); Active Lemur Tyrosine Kinase 3 (LMTK3); LMR3; TYKLM3; Serine/threonine-protein kinase LMTK3; LMTK3 active protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Freeze-dried powder
Sequence Length
1460
Applicable Applications for LMTK3 active protein
Cell culture, Activity Assays
Fragment
Leu133~Leu411
Tag
N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Organism Species
Human
Isoelectric Point
6.3
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Activity Data
(Lemur tyrosine kinase 3 (LMTK3), a member of the receptor tyrosine kinase (RTK) family, is implicated in breast cancer growth and endocrine resistance. It has been reported that LMTK3 promotes cell invasion, motility, and migration of the MCF-7 breast cancer cell line. To test the bioactivity of LMTK3, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of LMTK3. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C.Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown in Figure 1.)
Activity Data
(Lemur tyrosine kinase 3 (LMTK3), a member of the receptor tyrosine kinase (RTK) family, is implicated in breast cancer growth and endocrine resistance. It has been reported that LMTK3 promotes cell invasion, motility, and migration of the MCF-7 breast cancer cell line. To test the bioactivity of LMTK3, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of LMTK3. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C.Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown in Figure 1.)
SDS-Page
SDS-Page
Testing Data
Testing Data
Testing Data
Testing Data
Product Categories/Family for LMTK3 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
45.9kDa; 46kDa
NCBI Official Full Name
Serine/threonine-protein kinase LMTK3
NCBI Official Synonym Full Names
lemur tyrosine kinase 3
NCBI Official Symbol
LMTK3
NCBI Official Synonym Symbols
LMR3; TYKLM3; PPP1R101
NCBI Protein Information
serine/threonine-protein kinase LMTK3
UniProt Protein Name
Serine/threonine-protein kinase LMTK3
Protein Family
UniProt Gene Name
LMTK3
UniProt Synonym Gene Names
KIAA1883; TYKLM3
Uniprot Description
Lmr3: a dual-specificity kinase of the Lmr family.
Protein type: EC 2.7.11.1; Kinase, protein; Lmr family; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group
Chromosomal Location of Human Ortholog: 19q13.33
Research Articles on LMTK3
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Product Notes
The LMTK3 lmtk3 (Catalog #AAA2086088) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Lemur Tyrosine Kinase 3 (LMTK3) can be used in a range of immunoassay formats including, but not limited to, Cell culture, Activity Assays. Researchers should empirically determine the suitability of the LMTK3 lmtk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lemur Tyrosine Kinase 3 (LMTK3), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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