IDO Active Protein | IDO1 active protein

IDO, Recombinant, Mouse (His) (Indoleamine 2,3-dioxygenase, INDO)

Cat. Num. AAA637936

• Gene Names: IDO1; IDO; INDO; IDO-1
• Purity: Highly Purified; >=90% (SDS-PAGE)
• Synonyms: IDO; Recombinant; Mouse (His) (Indoleamine 2; 3-dioxygenase; INDO); IDO1 active protein

• Host: E.Coli
• Purity/Purification: Highly Purified; >=90% (SDS-PAGE)
• Form/Format: 0.2um-filtered solution in 50mM TRIS-Cl, pH 7.4, 1mM EDTA.
• Concentration: 1mg/ml (varies by lot)
• Sequence: Mouse IDO (aa 1-407) is fused at the C-terminus to a His-tag.

• Biological Activity: Specific Activity: >100,000U/mg protein with L-tryptophan as substrate. One unit is defined as the amount of enzyme that produces 1nmol of kynurenine per hour.
• Endotoxin: <1EU/ug (LAL)
• Heme Content: Not determined for end product. 7uM hermin was adde for protein induction.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months after receipt at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for IDO1 active protein

IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.

Recombinant protein corresponding to mouse IDO (aa1-407), at C-terminus fused to His-tag.

Product Categories/Family for IDO1 active protein

Molecular Biology; MB-Enzymes; Oxidase

NCBI and Uniprot Product Information

• NCBI GI #: 4504577
• NCBI GeneID: 3620
• NCBI Accession #: NP_002155.1
• NCBI GenBank Nucleotide #: NM_002164.5
• UniProt Accession #: P14902; Q540B4
• Molecular Weight: ~40kD (SDS-PAGE)
• NCBI Official Full Name: indoleamine 2,3-dioxygenase 1
• NCBI Official Synonym Full Names: indoleamine 2,3-dioxygenase 1
• NCBI Official Symbol: IDO1
• NCBI Official Synonym Symbols: IDO; INDO; IDO-1
• NCBI Protein Information: indoleamine 2,3-dioxygenase 1; indole 2,3-dioxygenase; indolamine 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase
• UniProt Protein Name: Indoleamine 2,3-dioxygenase 1
• Protein Family: Indoleamine 2,3-dioxygenase
• UniProt Gene Name: IDO1
• UniProt Synonym Gene Names: IDO; INDO; IDO-1
• UniProt Entry Name: I23O1_HUMAN

NCBI Description

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]

Uniprot Description

INDO: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. Belongs to the indoleamine 2,3-dioxygenase family.

Protein type: Amino Acid Metabolism - tryptophan; Cell cycle regulation; Oxidoreductase; EC 1.13.11.52

Chromosomal Location of Human Ortholog: 8p12-p11

Cellular Component: smooth muscle contractile fiber; stereocilium bundle; cytosol

Molecular Function: electron carrier activity; metal ion binding; heme binding; indoleamine 2,3-dioxygenase activity; tryptophan 2,3-dioxygenase activity

Biological Process: regulation of activated T cell proliferation; positive regulation of T-helper 2 type immune response; positive regulation of chronic inflammatory response; negative regulation of interleukin-10 production; positive regulation of interleukin-12 production; tryptophan catabolic process to kynurenine; response to lipopolysaccharide; female pregnancy; negative regulation of T cell proliferation; cytokine production during acute inflammatory response; tryptophan catabolic process; multicellular organismal response to stress; positive regulation of T cell tolerance induction