Sequence
Mdm2 p53 Binding Protein Homolog (MDM2) Active Protein | MDM2 active protein
Active Mdm2 p53 Binding Protein Homolog (MDM2)
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence
Sequence
Activity Data
(Mouse double minute 2 homolog (MDM2) also known as E3 ubiquitin-protein ligase Mdm2 is a cellular oncoprotein that recognizes the N-terminal trans-activation domain (TAD) of the p53 tumor suppressor and as an inhibitor of p53 transcriptional activation. The human homologue of this protein is sometimes called Hdm2. The p53 tumor suppressor is the key target of MDM2. It has been identified as a p53 interacting protein that represses p53 transcriptional activity and also acts as an E3 ubiquitin ligase, targeting both itself and p53 for degradation by the proteasome. MDM2 is capable of auto-polyubiquitination, and in complex with p300, a cooperating E3 ubiquitin ligase, is capable of polyubiquitinating p53. Besides, S100 Calcium Binding Protein (S100) has been identified as an interactor of MDM2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MDM2 and recombinant human S100. Briefly, MDM2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to S100-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MDM2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of MDM2 and S100 was shown in Figure 1, and this effect was in a dose dependent manner.)
Activity Data
(Mouse double minute 2 homolog (MDM2) also known as E3 ubiquitin-protein ligase Mdm2 is a cellular oncoprotein that recognizes the N-terminal trans-activation domain (TAD) of the p53 tumor suppressor and as an inhibitor of p53 transcriptional activation. The human homologue of this protein is sometimes called Hdm2. The p53 tumor suppressor is the key target of MDM2. It has been identified as a p53 interacting protein that represses p53 transcriptional activity and also acts as an E3 ubiquitin ligase, targeting both itself and p53 for degradation by the proteasome. MDM2 is capable of auto-polyubiquitination, and in complex with p300, a cooperating E3 ubiquitin ligase, is capable of polyubiquitinating p53. Besides, S100 Calcium Binding Protein (S100) has been identified as an interactor of MDM2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MDM2 and recombinant human S100. Briefly, MDM2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to S100-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MDM2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of MDM2 and S100 was shown in Figure 1, and this effect was in a dose dependent manner.)
Testing Data
(Gene Sequence (extract))
Testing Data
(Gene Sequence (extract))
SDS-Page
(SDS-PAGE Sample: Active recombinant MDM2, Human)
SDS-Page
(SDS-PAGE Sample: Active recombinant MDM2, Human)
Western Blot (WB)
(Western BlotSample: Recombinant MDM2, Human;Antibody: Rabbit Anti-Human MDM2 Ab (MBS2026105))
Western Blot (WB)
(Western BlotSample: Recombinant MDM2, Human;Antibody: Rabbit Anti-Human MDM2 Ab (MBS2026105))
NCBI and Uniprot Product Information
Predicted Molecular Weight: 39.7kDa
Accurate Molecular Weight: 44kDa
NCBI Description
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]
Uniprot Description
MDM2: a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 via Akt-mediated MDM2 phosphorylation. Phosphorylation of MDM2 increases its interaction with p300, providing a platform to allow the assembly of the protein complex necessary for MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. Facilitates the nuclear export of p53 and targets it for proteasome-mediated proteolysis. Eight alternatively spliced isoforms have been reported.
Protein type: EC 6.3.2.-; EC 6.3.2.19; Inhibitor; Ligase; Nucleolus; Oncoprotein; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 12q15
Cellular Component: cytoplasm; cytosol; nuclear body; nucleolus; nucleoplasm; nucleus; plasma membrane; protein complex; synapse
Molecular Function: 5S rRNA binding; enzyme binding; identical protein binding; ligase activity; p53 binding; peroxisome proliferator activated receptor binding; protein binding; protein domain specific binding; protein N-terminus binding; ribonucleoprotein complex binding; SUMO transferase activity; ubiquitin protein ligase binding; ubiquitin-protein transferase activity; zinc ion binding
Biological Process: amyloid fibril formation; atrial septum development; atrioventricular valve morphogenesis; blood vessel development; blood vessel remodeling; cellular response to alkaloid; cellular response to estrogen stimulus; cellular response to gamma radiation; cellular response to growth factor stimulus; cellular response to hydrogen peroxide; cellular response to hypoxia; cellular response to peptide hormone stimulus; cellular response to UV-C; cellular response to vitamin B1; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; endocardial cushion morphogenesis; establishment of protein localization; negative regulation of cell cycle arrest; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator; negative regulation of neuron projection development; negative regulation of protein processing; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; peptidyl-lysine modification; positive regulation of cell proliferation; positive regulation of gene expression; positive regulation of mitotic cell cycle; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein export from nucleus; positive regulation of vascular associated smooth muscle cell migration; positive regulation of vascular smooth muscle cell proliferation; protein autoubiquitination; protein complex assembly; protein destabilization; protein deubiquitination; protein kinase B signaling; protein localization to nucleus; protein sumoylation; protein ubiquitination; protein ubiquitination involved in ubiquitin-dependent protein catabolic process; proteolysis involved in cellular protein catabolic process; regulation of heart rate; regulation of protein catabolic process; response to antibiotic; response to cocaine; response to drug; response to ether; response to formaldehyde; response to iron ion; response to magnesium ion; response to morphine; response to steroid hormone; response to toxin; traversing start control point of mitotic cell cycle; ventricular septum development; viral process
Disease: Accelerated Tumor Formation, Susceptibility To
Research Articles on MDM2
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Product Notes
The MDM2 mdm2 (Catalog #AAA2097320) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Mdm2 p53 Binding Protein Homolog (MDM2) can be used in a range of immunoassay formats including, but not limited to, Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the MDM2 mdm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mdm2 p53 Binding Protein Homolog (MDM2), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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