Activity Data
(JAK2 (Tyrosine-protein kinase JAK2) is a tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. JAK2 is considered to associate with some type I receptors, including EPOR (Erythropoietin receptor), therefore participates in cellular signal transduction. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human JAK2 and recombinant human EPOR. Briefly, JAK2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL JAK2 were then transferred to EPOR-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-JAK2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of JAK2 and EPOR was shown in Figure 1, and this effect was in a dose dependent manner.)
Janus Kinase 2 (JAK2) Active Protein | JAK2 active protein
Active Janus Kinase 2 (JAK2)
Activity Data
(JAK2 (Tyrosine-protein kinase JAK2) is a tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. JAK2 is considered to associate with some type I receptors, including EPOR (Erythropoietin receptor), therefore participates in cellular signal transduction. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human JAK2 and recombinant human EPOR. Briefly, JAK2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL JAK2 were then transferred to EPOR-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-JAK2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of JAK2 and EPOR was shown in Figure 1, and this effect was in a dose dependent manner.)
Activity Data
(JAK2 (Tyrosine-protein kinase JAK2) is a tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. JAK2 is considered to associate with some type I receptors, including EPOR (Erythropoietin receptor), therefore participates in cellular signal transduction. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human JAK2 and recombinant human EPOR. Briefly, JAK2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL JAK2 were then transferred to EPOR-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-JAK2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of JAK2 and EPOR was shown in Figure 1, and this effect was in a dose dependent manner.)
SDS-Page
SDS-Page
Testing Data
Testing Data
Testing Data
Testing Data
NCBI and Uniprot Product Information
Accurate Molecular Weight: 35kDa
NCBI Description
This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008]
Uniprot Description
JAK2: a non-receptor tyrosine-kinase involved in a specific subset of cytokine receptor signaling pathways, including IL-3, -5 and GM-CSF. Interacts with IL23R, SKB1 and STAM2. It has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. Fusion of Jak2 to TEL1 (ETV6) by t(9;12)(p24;p13) causes myeloproliferative disease in humans and mouse models. The Jak inhibitor AG490 inhibits constitutive Jak2 phosphorylation and causes apoptosis in cells from breast cancer and relapsing acute lymphoblastic leukemia. A single activating mutation is associated with several hematological malignancies. Inhibitor: AG490.
Protein type: EC 2.7.10.2; JakA family; Kinase, protein; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group
Chromosomal Location of Human Ortholog: 9p24.1
Cellular Component: caveola; cytoplasm; cytoskeleton; cytosol; extrinsic component of cytoplasmic side of plasma membrane; focal adhesion; membrane raft; nuclear matrix; nucleoplasm; nucleus; plasma membrane
Molecular Function: acetylcholine receptor binding; ATP binding; growth hormone receptor binding; heme binding; histone binding; identical protein binding; insulin receptor substrate binding; interleukin-12 receptor binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; peptide hormone receptor binding; phosphoinositide 3-kinase binding; protein binding; protein C-terminus binding; protein kinase activity; protein kinase binding; protein tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor binding; SH2 domain binding; type 1 angiotensin receptor binding
Biological Process: actin filament polymerization; activation of cysteine-type endopeptidase activity involved in apoptotic process; activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway; activation of JAK protein; activation of MAPKK activity; adaptive immune response; apoptosis; axon regeneration; blood coagulation; cell differentiation; cell migration; cell motility; cytokine-mediated signaling pathway; elevation of cytosolic calcium ion concentration; enzyme linked receptor protein signaling pathway; erythrocyte differentiation; G-protein coupled receptor signaling pathway; induction of apoptosis by oxidative stress; innate immune response; JAK-STAT cascade; MAPK cascade; mesoderm development; mineralocorticoid receptor signaling pathway; negative regulation of cardiac muscle cell apoptotic process; negative regulation of cell proliferation; negative regulation of cell-cell adhesion; negative regulation of DNA binding; negative regulation of heart contraction; negative regulation of neuron apoptosis; peptidyl-tyrosine autophosphorylation; peptidyl-tyrosine phosphorylation; platelet-derived growth factor receptor signaling pathway; positive regulation of cell activation; positive regulation of cell differentiation; positive regulation of cell migration; positive regulation of cell-substrate adhesion; positive regulation of DNA binding; positive regulation of epithelial cell apoptotic process; positive regulation of growth factor dependent skeletal muscle satellite cell proliferation; positive regulation of inflammatory response; positive regulation of insulin secretion; positive regulation of interleukin-1 beta production; positive regulation of nitric oxide biosynthetic process; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of phosphoprotein phosphatase activity; positive regulation of protein import into nucleus, translocation; positive regulation of transcription factor activity; positive regulation of tumor necrosis factor production; positive regulation of tyrosine phosphorylation of STAT protein; positive regulation of vascular smooth muscle cell proliferation; protein amino acid phosphorylation; protein autophosphorylation; regulation of apoptosis; regulation of cell proliferation; regulation of inflammatory response; response to antibiotic; response to hydroperoxide; response to lipopolysaccharide; response to tumor necrosis factor; signal transduction; STAT protein import into nucleus; tumor necrosis factor-mediated signaling pathway; tyrosine phosphorylation of STAT protein
Disease: Budd-chiari Syndrome; Erythrocytosis, Familial, 1; Myelofibrosis; Polycythemia Vera; Thrombocythemia 3
Research Articles on JAK2
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Product Notes
The JAK2 jak2 (Catalog #AAA2097244) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The Active Janus Kinase 2 (JAK2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Janus Kinase 2 (JAK2) can be used in a range of immunoassay formats including, but not limited to, Cell culture, Activity Assays. Researchers should empirically determine the suitability of the JAK2 jak2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Janus Kinase 2 (JAK2), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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