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Type
Reactivity
Gene Name
Isotype
Host
Application
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2533 results for " His Active" - showing 300-350
Activity Data
(Figure. The binding activity of IGFBP2 with IGFBP2.Insulin Like Growth Factor Binding Protein 2 (IGFBP2) is a menber of IGF binding proteins (IGFBPs) family. IGF binding proteins (IGFBPs) are proteins of 24 to 45kDa. Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Besides, Insulin Like Growth Factor 2 (IGF2) has been identified as an interactor of IGFBP2, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IGFBP2 and recombinant mouse IGF2. Briefly, IGFBP2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to IGF2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IGFBP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IGFBP2 and IGFBP2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Insulin Like Growth Factor Binding Protein 2 (IGFBP2), Active Protein (Cat# AAA2105065)
Full Name
Active Insulin Like Growth Factor Binding Protein 2 (IGFBP2)
Gene Names
Igfbp2; IBP-2; Igfbp-2; AI255832; mIGFBP-2
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$180/0.01 mg | $345/0.05 mg | $530/0.1 mg | $650/0.2 mg | $1,260/0.5 mg | $1,870/1 mg | $4,595/5 mg
SDS-Page
(Fig.SDS-PAGE analysis of Human RANKL protein, Fc Tag.)
RANKL, Active Protein (Cat# AAA9718700)
Full Name
Human RANKL Protein, Fc Tag
Gene Names
TNFSF11; ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2
Purity
>90% as determined by SDS-PAGE
Pricing
$345/0.02 mg | $1,220/0.1 mg | $185/0.005 mg | $5,485/5x0.1 mg
Activity Data
(Figure1. TRAP levels in the cell of RAW264.7 cells induced by RANKL.Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is a member of the tumor necrosis factor (TNF) superfamily. RANKL has been identified to affect the immune system and control bone regeneration and remodeling. RANKL is an apoptosis regulator gene, a binding partner of osteoprotegerin (OPG), a ligand for the receptor RANK and controls cell proliferation by modifying protein levels of Id4, Id2 and cyclin D1. The protein can bind to RANK on cells of the myeloid lineage and functions as a key factor for osteoclast differentiation and activation. Thus, we use recombinant RANKL to induce RAW264.7 mouse monocyte/macrophage cells to differentiate to osteoclast cell. RAW264.7 mouse monocyte/macrophage cells were seeded into 24-well plates at a density of 2×105 and allowed to attach overnight, then treated with RANKL (1ng/mL, 10ng/mL) and incubated for 72h. Then we assay TRAP (a kind of enzyme expressed by osteoclast cell) by ELASA. Result: TRAP levels in the cell supernatant of RAW264.7 cells increased significantly after stimulated with RANK, the data was shown in Table 1 and Figure1.)
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL), Active Protein (Cat# AAA2105123)
Full Name
Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
Gene Names
Tnfsf11; ODF; OPGL; RANKL; Ly109l; Trance
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
Activity Data
(Figure. The binding activity of GDNF with GFRa2.Glial cell-derived neurotrophic factor (GDNF) is a small protein that potently promotes the survival of many types of neurons. This protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The most prominent feature of GDNF is its ability to support the survival of dopaminergic and motorneurons. Besides, Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 2 (GFRa2) has been identified as an interactor of GDNF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse GDNF and recombinant mouse GFRa2. Briefly, GDNF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GFRa2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GDNF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of GDNF and GFRa2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Glial Cell Line Derived Neurotrophic Factor (GDNF), Active Protein (Cat# AAA2105059)
Full Name
Active Glial Cell Line Derived Neurotrophic Factor (GDNF)
Gene Names
Gdnf; ATF; AI385739
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$230/0.01 mg | $500/0.05 mg | $770/0.1 mg | $955/0.2 mg | $1,860/0.5 mg | $2,755/1 mg | $6,740/5 mg
SDS-Page
PRDX4, Active Protein (Cat# AAA203650)
Full Name
PRDX4, 38-271aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
PRDX4; PRX-4; AOE372; AOE37-2; HEL-S-97n
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
$200/0.02 mg | $315/0.1 mg | $680/0.5 mg | $2,820/5x0.5 mg
Activity Data
(Perforin 1 (PRF1) is a pore forming cytolytic protein found in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin binds to the target cell's plasma membrane, and oligomerises in a Ca2+ dependent manner to form pores on the target cell. Perforin is thought to act by creating holes in the plasma membrane which triggers an influx of calcium and initiates membrane repair mechanisms. These repair mechanisms bring perforin and granzymes into early endosomes. Besides, Granzyme A (GZMA) has been identified as an interactor of PRF1, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse PRF1 and recombinant mouse GZMA. Briefly, PRF1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to GZMA-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PRF1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PRF1 and GZMA was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The Binding Activity of PRF1 with GZMA.)
Perforin 1 (PRF1), Active Protein (Cat# AAA2104734)
Full Name
Active Perforin 1 (PRF1)
Reactivity
Mus musculus (Mouse)
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
>92%
Pricing
$230/0.01 mg | $495/0.05 mg | $765/0.1 mg | $945/0.2 mg | $1,845/0.5 mg | $2,730/1 mg | $6,670/5 mg
SDS-Page
Cyclophilin F, Active Protein (Cat# AAA203258)
Full Name
Cyclophilin F (PPIF), 30-207aa, Human,His tag, E Coli (Bioactivity Validated)
Gene Names
PPIF; CYP3; CypD; CyP-M; Cyp-D
Applications
SDS-PAGE
Purity
> 95% by SDS PAGE
Pricing
$190/0.02 mg | $280/0.1 mg | $570/0.5 mg | $2,325/5x0.5 mg
Activity Data
(IL2 (Interleukin-2) is a cytokine produced by T-cells in response to antigenic or mitogenic stimulation. IL2 is a type of signaling molecule in the immune system, that is required for both T-cell and B-cell proliferation and other activities crucial to regulation of the immune response. Recombinant human IL2 shares 56% AA sequence identity with mouse IL2, suggesting the exist of cross-species activity. Therefore, in order to detect the bioactivity of rhIL2, a cell proliferation assay has been conducted using CTLL-2 mouse cytotoxic T cells. Briefly, CTLL-2 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with or without the addition of various concentrations of IL2. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). 10uL of CCK-8 solution was added to each well of the plate, the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of CTLL-2 cells after incubation with IL2 for 48h observed by inverted microscope was shown in Figure 1.)
Interleukin 2 (IL2), Active Protein (Cat# AAA2097325)
Full Name
Active Interleukin 2 (IL2)
Gene Names
IL2; IL-2; TCGF; lymphokine
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$215/0.01 mg | $450/0.05 mg | $690/0.1 mg | $850/0.2 mg | $1,660/0.5 mg | $2,470/1 mg | $6,035/5 mg
SDS-Page
(3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Beta-lactaminase, Active Protein (Cat# AAA206625)
Full Name
Beta-lactaminase, E Coli, 20-377, His-tag, E Coli (Bioactivity Validated)
Gene Names
ampC; ampA; ECK4146; JW4111
Applications
SDS-PAGE, Enzyme Activity
Purity
> 95% by SDS-PAGE
Pricing
$190/0.01 mg | $230/0.02 mg | $290/0.05 mg | $405/0.1 mg | $985/0.5 mg | $630/0.25 mg | $4,180/5x0.5 mg
SDS-Page
Interleukin-3, Active Protein (Cat# AAA203126)
Full Name
Interleukin-3, 20-152aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
IL3; IL-3; MCGF; MULTI-CSF
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
$230/0.02 mg | $405/0.1 mg | $985/0.5 mg | $4,180/5x0.5 mg
Activity Data
(IFN-gamma is a dimerized soluble cytokine that is the only member of the type II class of interferons.The importance of IFNgamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. As reported, IFNgamma is an important activator of human monocytic THP1 cells. Therefore,THP-1 cells were incubated in RPMI 1640 with various concentration of IFN-gamma, then cells were observed by inverted microscope everyday.After stimulated with IFN-gamma(5ng/ml) for 5 days, morphological changes occured in THP1 cells which displayed the shape of fusiform or polygon and were more likely to adhere.Effect of IFN-gamma on THP1 cells is shown in Figure 1.)
Interferon Gamma (IFNg), Active Protein (Cat# AAA2086058)
Full Name
Active Interferon Gamma (IFNg)
Gene Names
IFNG; IFG; IFI
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$130/0.01 mg | $215/0.05 mg | $295/0.1 mg | $365/0.2 mg | $685/0.5 mg | $1,010/1 mg | $2,450/5 mg | $11,025/5x5 mg
Activity Data
(Figure. The binding activity of BDNF with APP.Brain-derived neurotrophic factor, also known as BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical Nerve Growth Factor. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. Besides, Amyloid Precursor Protein (APP) has been identified as an interactor of BDNF, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat BDNF and recombinant rat APP. Briefly, BDNF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to APP-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-BDNF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of BDNF and APP was shown in Figure 1, and this effect was in a dose dependent manner.)
Brain Derived Neurotrophic Factor (BDNF), Active Protein (Cat# AAA2105048)
Full Name
Active Brain Derived Neurotrophic Factor (BDNF)
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$250/0.01 mg | $540/0.05 mg | $840/0.1 mg | $1,045/0.2 mg | $2,040/0.5 mg | $3,020/1 mg | $7,385/5 mg
Activity Data
(Figure. The binding activity of TNNI3 with TNNT2.Troponin I Type 3, Cardiac (TNNI3) is a protein that in humans is encoded by the TNNI3 gene. It is a tissue-specific subtype of troponin I, which in turn is a part of the troponin complex. The TNNI3 gene encoding cardiac troponin I (cTnI) is located at 19q13.4 in the human chromosomal genome. Human cTnI is a 24kDa protein consisting of 210 amino acids with isoelectric point (pI) of 9.87. cTnI is exclusively expressed in adult cardiac muscle. Besides, Troponin T Type 2, Cardiac (TNNT2) has been identified as an interactor of TNNI3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TNNI3 and recombinant human TNNT2. Briefly, TNNI3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to TNNT2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TNNI3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TNNI3 and TNNT2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Troponin I Type 3, Cardiac (TNNI3), Active Protein (Cat# AAA2105094)
Full Name
Active Troponin I Type 3, Cardiac (TNNI3)
Gene Names
TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$205/0.01 mg | $405/0.05 mg | $625/0.1 mg | $770/0.2 mg | $1,495/0.5 mg | $2,225/1 mg | $5,435/5 mg
Activity Data
(Figure. The binding activity of S100A8 with S100A9.S100 calcium-binding protein A8 (S100A8) also known as calgranulin A, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Besides, S100 Calcium Binding Protein A9 (S100A9) has been identified as an interactor of S100A8, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat S100A8 and recombinant rat S100A9. Briefly, S100A8 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to S100A9-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100A8 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of S100A8 and S100A9 was shown in Figure 1, and this effect was in a dose dependent manner.)
S100 Calcium Binding Protein A8 (S100A8), Active Protein (Cat# AAA2105152)
Full Name
Active S100 Calcium Binding Protein A8 (S100A8)
Gene Names
S100a8; Mrp8
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$180/0.01 mg | $345/0.05 mg | $530/0.1 mg | $645/0.2 mg | $1,250/0.5 mg | $1,860/1 mg | $4,570/5 mg
SDS-Page
uGDH, Active Protein (Cat# AAA203653)
Full Name
uGDH, 1-494aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
UGDH; GDH; UGD; UDPGDH; UDP-GlcDH
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
$215/0.01 mg | $340/0.05 mg | $765/0.25 mg | $3,195/5x0.25 mg
Activity Data
(JAK3 (Tyrosine-protein kinase JAK3) is a non-receptor tyrosine kinase that involved in various processes such as cell growth, development, or differentiation. Besides, STAM2 has been identified as an interactor of JAK3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human JAK3 and recombinant human STAM2. Briefly, JAK3 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAM2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-JAK3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of JAK3 and STAM2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Janus Kinase 3 (JAK3), Active Protein (Cat# AAA2097317)
Full Name
Active Janus Kinase 3 (JAK3)
Gene Names
JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$255/0.01 mg | $565/0.05 mg | $875/0.1 mg | $1,080/0.2 mg | $2,120/0.5 mg | $3,145/1 mg | $7,695/5 mg
Activity Data
(The main role of factor VII (FVII) is to initiate the process of coagulation in conjunction with tissue factor (TF). Tissue factor is found on the outside of blood vessels-normally not exposed to the bloodstream. Upon vessel injury, tissue factor is exposed to the blood and circulating FVII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. Tissue Factor (TF) has been identified as an interactor of FVII, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat FVII and recombinant rat TF. Briefly, FVII were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TF-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FVII pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of FVII and TF was shown in Figure 1, and this effect was in a dose dependent manner.)
Coagulation Factor VII (F7), Active Protein (Cat# AAA2097300)
Full Name
Active Coagulation Factor VII (F7)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$270/0.01 mg | $600/0.05 mg | $930/0.1 mg | $1,145/0.2 mg | $2,260/0.5 mg | $3,350/1 mg | $8,205/5 mg
Activity Data
(Figure. The binding activity of IL8 with SDC1.Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cellsendothelial cells. IL-8, also known as neutrophil chemotactic factor, has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL8 also induces phagocytosis once they have arrived. IL8 is also known to be a potent promoter of angiogenesis. Besides, Syndecan 1 (SDC1) has been identified as an interactor of IL8, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL8 and recombinant human SDC1. Briefly, IL8 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SDC1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL8 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL8 and SDC1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interleukin 8 (IL8), Active Protein (Cat# AAA2105072)
Full Name
Active Interleukin 8 (IL8)
Gene Names
CXCL8; IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$150/0.01 mg | $255/0.05 mg | $385/0.1 mg | $470/0.2 mg | $895/0.5 mg | $1,315/1 mg | $3,210/5 mg | $14,445/5x5 mg
SDS-Page
ACPP, Active Protein (Cat# AAA206396)
Full Name
ACPP, 33-386aa, Human, His-tag, Baculovirus (Bioactivity Validated)
Gene Names
ACPP; ACP3; 5'-NT; ACP-3
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
$230/0.01 mg | $300/0.02 mg | $405/0.05 mg | $595/0.1 mg | $1,585/0.5 mg | $985/0.25 mg | $6,880/5x0.5 mg
SDS-Page
GSR, Active Protein (Cat# AAA204266)
Full Name
GSR, 43-522aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
GSR; HEL-75; HEL-S-122m
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
$215/0.01 mg | $340/0.05 mg | $765/0.25 mg | $3,195/5x0.25 mg
Activity Data
(Figure. The binding activity of ODC with TK1.The enzyme ornithine decarboxylase (ODC) catalyzes the decarboxylation of ornithine (a product of the urea cycle) to form putrescine. This reaction is the committed step in polyamine synthesis. Lysine 69 on ornithine decarboxylase (ODC) binds the cofactor pyridoxal phosphate to form a Schiff base. Ornithine displaces the lysine to form a Schiff base attached to ODC, which decarboxylates to form a quinoid intermediate. This intermediate rearranges to form a Schiff base attached to putrescine, which is attacked by lysine to release putrescine product and reform PLP-bound ODC. Besides, Thymidine Kinase 1, Soluble (TK1) has been identified as an interactor of ODC, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat ODC and recombinant rat TK1. Briefly, ODC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TK1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ODC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ODC and TK1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Ornithine Decarboxylase (ODC), Active Protein (Cat# AAA2105138)
Full Name
Active Ornithine Decarboxylase (ODC)
Gene Names
Odc1; Odc
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$245/0.01 mg | $530/0.05 mg | $820/0.1 mg | $1,020/0.2 mg | $1,985/0.5 mg | $2,945/1 mg | $7,200/5 mg
Activity Data
((FGF1) Fibroblast growth factor 1 belongs to the fibroblast growth factor (FGF) family. FGF1 plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. FGF1 is thought to stimulate the proliferation of 3T3 fibroblasts. Thus, a cell proliferation assay was conducted to detect the bioactivity of recombinant mouse FGF1 using 3T3 fibroblasts. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF1. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of 3T3 cells after incubation with FGF1 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF1 for 48h. The result was shown in Figure 2. It was obvious that FGF1 significantly increased cell viability of 3T3 cells.)
Fibroblast Growth Factor 1, Acidic (FGF1), Active Protein (Cat# AAA2097255)
Full Name
Active Fibroblast Growth Factor 1, Acidic (FGF1)
Gene Names
Fgf1; Fam; Fgfa; Dffrx; Fgf-1
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$130/0.01 mg | $220/0.05 mg | $305/0.1 mg | $375/0.2 mg | $705/0.5 mg | $1,045/1 mg | $2,540/5 mg | $11,420/5x5 mg
Activity Data
(BMP4 (Bone morphogenetic protein 4) is a member of the bone morphogenetic protein family, which is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been proven that HJV (Hemojuvelin) acts as a coreceptor of BMPs, including BMP4; therefore, a binding ELISA assay was constructed to detect the association of recombinant human BMP4 with recombinant human HJV. Briefly, BMP4 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HJV-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-BMP4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of BMP4 with HJV was shown in Figure 1 and this effect was in a dose dependent manner.)
Bone Morphogenetic Protein 4 (BMP4), Active Protein (Cat# AAA2097338)
Full Name
Active Bone Morphogenetic Protein 4 (BMP4)
Gene Names
BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
Sequence
Collagen Type VI Alpha 1 (COL6a1), Active Protein (Cat# AAA2105170)
Full Name
Active Collagen Type VI Alpha 1 (COL6a1)
Gene Names
Col6a1; Col6a-1; AI747156
Reactivity
Mus musculus (Mouse)
Applications
Cell culture; Activity Assays.
Purity
>95%
Pricing
$225/0.01 mg | $490/0.05 mg | $755/0.1 mg | $940/0.2 mg | $1,830/0.5 mg | $2,710/1 mg | $6,620/5 mg
Sequence
Inter Alpha-Globulin Inhibitor H4 (ITIH4), Active Protein (Cat# AAA2105190)
Full Name
Active Inter Alpha-Globulin Inhibitor H4 (ITIH4)
Gene Names
ITIH4; H4P; IHRP; GP120; PK120; ITIHL1; PK-120; ITI-HC4
Reactivity
Homo sapiens (Human)
Applications
Cell Culture, Activity Assays
Purity
>98%
Pricing
$220/0.01 mg | $460/0.05 mg | $705/0.1 mg | $875/0.2 mg | $1,705/0.5 mg | $2,540/1 mg | $6,205/5 mg
Vascular Endothelial Growth Factor, Active Protein (Cat# AAA143537)
Full Name
Recombinant Mouse Vascular Endothelial Growth Factor, His Tag
Gene Names
Vegfa; Vpf; Vegf
Purity
Greater than 90.0% as determined by SDS-PAGE.
Pricing
$250/0.01 mg | $5,300/1 mg | $180/0.002 mg | $23,620/5x1 mg
Activity Data
(Figure. The binding activity of SIRT3 with IDH2.Sirtuin 3 (SIRT3), the NAD-dependent deacetylaseis is member of the mammalian sirtuin family of proteins. In human, sirtuins have a range of molecular functions and have emerged as important proteins in aging, stress resistance and metabolic regulation. It also can regulate epigenetic gene silencing and suppress recombination of rDNA in yeast. SIRT3 expression in white and brown adipose tissue. Besides, Isocitrate Dehydrogenase 2, mitochondrial (IDH2) has been identified as an interactor of SIRT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat SIRT3 and recombinant rat IDH2. Briefly, SIRT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IDH2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-SIRT3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of SIRT3 and IDH2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Sirtuin 3 (SIRT3), Active Protein (Cat# AAA2097316)
Full Name
Active Sirtuin 3 (SIRT3)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$230/0.01 mg | $500/0.05 mg | $770/0.1 mg | $955/0.2 mg | $1,860/0.5 mg | $2,755/1 mg | $6,740/5 mg
SDS-Page
ADA, Active Protein (Cat# AAA204402)
Full Name
ADA, 1-363aa, Human, His tag, E Coli (Bioactivity Validated)
Applications
SDS-PAGE
Purity
> 85% by SDS-PAGE
Pricing
$230/0.01 mg | $405/0.05 mg | $985/0.25 mg | $4,180/5x0.25 mg
Glutamic-Pyruvate Transaminase, Active Protein (Cat# AAA146771)
Full Name
Recombinant Human Glutamic-Pyruvate Transaminase, His Tag, Active
Gene Names
GPT; AAT1; ALT1; GPT1
Purity
Greater than 90% as determined by SDS-PAGE.
Pricing
$250/0.01 mg | $6,100/1 mg | $180/0.002 mg | $27,220/5x1 mg
Activity Data
(Plasminogen (Plg) can be converted into active plasmin by tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), kallikrein, and factor XII (Hageman factor). Plasmin can dissolve fibrin blood clots, act on many other processes such as embryonic development, tissue remodeling, inflammation and tumor invasion. Plasmint also can activates collagenases, weakens the walls of the Graafian follicle, cleaves fibrin, fibronectin, thrombospondin, laminin, and von Willebrand factor. Besides, Actin Beta (ACTb) has been identified as an interactor of Plg, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Plg and recombinant rat ACTb. Briefly, Plg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-Plg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of Plg and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.)
Plasminogen (Plg), Active Protein (Cat# AAA2097291)
Full Name
Active Plasminogen (Plg)
Gene Names
Plg; Ab1-346
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$215/0.01 mg | $450/0.05 mg | $690/0.1 mg | $850/0.2 mg | $1,660/0.5 mg | $2,470/1 mg | $6,035/5 mg
Adiponectin (Acrp30), Active Protein (Cat# AAA691926)
Full Name
Mouse Adiponectin (Acrp30)
Gene Names
Adipoq; APN; Acdc; apM1; 30kDa; GBP28; adipo; Acrp30
Purity
> 98% by SDS-PAGE gel and HPLC analyses.
Pricing
$395/0.025 mg | $250/0.005 mg | $1,565/5x0.025 mg
GPX1, Active Protein (Cat# AAA636620)
Full Name
GPX1, Recombinant, Human (His) (Glutathione Peroxidase 1, Cellular Glutathione Peroxidase, GPx-1, GSHPx-1)
Gene Names
GPX1; GPXD; GSHPX1
Purity
Highly Purified
>90% (SDS-PAGE)
>90% (SDS-PAGE)
Pricing
$500/0.01 mg | $2,250/5x0.01 mg
FGF-23, Active Protein (Cat# AAA635588)
Full Name
FGF-23, Recombinant, Human (His) (Fibroblast Growth Factor 23, Tumor-derived Hypophosphatemia-inducing Factor)
Gene Names
FGF23; ADHR; FGFN; HYPF; HPDR2; PHPTC
Purity
Highly Purified
>90% (SDS-PAGE)
>90% (SDS-PAGE)
Pricing
$480/0.01 mg | $2,145/5x0.01 mg
Activity Data
(Figure. The apoptosis of A549 cells by TNFalpha was inhibited by TNFRSF1B.Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B), also known as tumor necrosis factor receptor 2 (TNFR2) and CD120b, is a membrane receptor that binds tumor necrosis factor-alpha (TNFalpha). This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. TNFRSF1B can inhibit cell apoptosis which induced by TNFalpha. Briefly, A549 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM including 1mug/mL TNFalpha and various concentrations of recombinant human TNFRSF1B. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate at 37 degree C for 1-4 hours. Apoptosis of A549 cells had been inhibit after incubation with TNFRSF1B for 96h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant TNFRSF1B for 96h. The result was shown in Figure 2. It was obvious that TNFRSF1B significantly suppress cell apoptosis induced by TNFalpha.(A) A549 cells cultured in DMEM contain 1mug/mL TNFalpha and 0.1ng/mL TNFRSF1B for 96h; (B) A549 cells cultured in DMEM only contain 1mug/mL TNFalpha for 96h.)
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A), Active Protein (Cat# AAA2105140)
Full Name
Active Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A)
Gene Names
TNFRSF1A; FPF; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$185/0.01 mg | $365/0.05 mg | $560/0.1 mg | $685/0.2 mg | $1,330/0.5 mg | $1,975/1 mg | $4,855/5 mg
Sequence
Granulin (GRN), Recombinant Protein (Cat# AAA2011118)
Full Name
Recombinant Granulin (GRN)
Gene Names
GRN; GEP; GP88; PEPI; PGRN; CLN11; PCDGF
Reactivity
Homo sapiens (Human)
Applications
WB
Purity
> 95%
Pricing
$115/0.01 mg | $175/0.05 mg | $230/0.1 mg | $275/0.2 mg | $505/0.5 mg | $735/1 mg | $1,775/5 mg | $7,975/5x5 mg
Activity Data
(TNFRSF12A (Tumor necrosis factor receptor superfamily member 12A) belongs to the Tumor necrosis factor receptor superfamily. TNFRSF12A is thought to induce apoptosis weakly in some cell types and promotes angiogenesis and the proliferation of endothelial cells. A binding ELISA assay was conducted to detect the association of TNFRSF12A with TNFa. Briefly, TNFRSF12A were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TNFRSF12A were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF12A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF12A and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.)
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A), Active Protein (Cat# AAA2097282)
Full Name
Active Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A)
Gene Names
TNFRSF12A; FN14; CD266; TWEAKR
Applications
Cell culture, Activity Assays
Purity
>85%
Pricing
$250/0.01 mg | $550/0.05 mg | $855/0.1 mg | $1,055/0.2 mg | $2,075/0.5 mg | $3,075/1 mg | $7,525/5 mg
Sequence
High Mobility Group Protein 1 (HMG1), Active Protein (Cat# AAA2105091)
Full Name
Active High Mobility Group Protein 1 (HMG1)
Gene Names
HMGB1; HMG1; HMG3; HMG-1; SBP-1
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
>95%
Pricing
$250/0.01 mg | $555/0.05 mg | $865/0.1 mg | $1,065/0.2 mg | $2,095/0.5 mg | $3,100/1 mg | $7,590/5 mg
CD27 ligand, soluble, Active Protein (Cat# AAA691798)
Full Name
Human CD27 ligand, soluble
Gene Names
CD70; CD27L; CD27LG; TNFSF7
Reactivity
Human
Purity
> 95% by SDS-PAGE gel and HPLC analyses.
Pricing
$250/0.01 mg | $395/0.05 mg | $1,565/5x0.05 mg
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
GOT2, Active Protein (Cat# AAA201147)
Full Name
Recombinant GOT2
Gene Names
Got2; Got-2; Kyat4; mAspAT; FABP-pm; AL022787
Applications
SDS-PAGE, Enzyme Activity
Purity
>90% by SDS-PAGE
Pricing
$550/0.05 mg | $690/0.1 mg | $2,865/5x0.1 mg
Activity Data
(Figure. The binding activity of DKK1 with CTNNb1.)
Dickkopf Related Protein 1 (DKK1), Active Protein (Cat# AAA2097279)
Full Name
Active Dickkopf Related Protein 1 (DKK1)
Gene Names
DKK1; SK; DKK-1
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$230/0.01 mg | $500/0.05 mg | $775/0.1 mg | $960/0.2 mg | $1,870/0.5 mg | $2,770/1 mg | $6,775/5 mg
SDS-Page
Thioredoxin 1, Active Protein (Cat# AAA203506)
Full Name
Thioredoxin 1, 2-109aa, E Coli, His tag, E Coli (Bioactivity Validated)
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
$215/0.01 mg | $340/0.05 mg | $765/0.25 mg | $3,195/5x0.25 mg
Activity Data
(Figure. The binding activity of IAP with THBS1.)
Integrin Associated Protein (IAP), Active Protein (Cat# AAA2097286)
Full Name
Active Integrin Associated Protein (IAP)
Gene Names
Cd47; IAP; Itgp; AA407862; AI848868; AW108519; 9130415E20Rik; B430305P08Rik
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$215/0.01 mg | $450/0.05 mg | $690/0.1 mg | $850/0.2 mg | $1,660/0.5 mg | $2,470/1 mg | $6,035/5 mg
Activity Data
(Figure. The binding activity of S100B with RAGE.Protein S100B is a member of the S100 family. S100 proteins are EF-hand calcium-binding proteins and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Experimental results suggest that the receptor for advanced glycation end products (RAGE) plays important roles in mediating S100 protein-induced cellular signaling. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human S100B and recombinant human RAGE. Briefly, S100B were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100B mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of S100B and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.)
S100 Calcium Binding Protein B (S100B), Active Protein (Cat# AAA2105105)
Full Name
Active S100 Calcium Binding Protein B (S100B)
Gene Names
S100B; NEF; S100; S100-B; S100beta
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$195/0.01 mg | $390/0.05 mg | $600/0.1 mg | $735/0.2 mg | $1,425/0.5 mg | $2,120/1 mg | $5,215/5 mg
Activity Data
(Nucb2 (Nucleobindin-2), a calcium-binding protein, is further cleaved into NES1 (Nesfatin-1). NES1 is an anorexigenic peptide and seems to participate in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. GADD45A (Growth arrest and DNA damage-inducible protein GADD45 alpha) has been identified as an interactor of Nucb2. Besides, rat GADD45A shares similarities with mouse NES1 in amino acid sequence with the identity of 98%. Thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse NES1 and recombinant rat GADD45A. Briefly, NES1 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL NES1 were then transferred to GADD45A-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-NES1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of NES1 and GADD45A was shown in Figure 1, and this effect was in a dose dependent manner.)
Nesfatin 1 (NES1), Active Protein (Cat# AAA2097271)
Full Name
Active Nesfatin 1 (NES1)
Gene Names
Nucb2; p54; Nefa
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$255/0.01 mg | $565/0.05 mg | $875/0.1 mg | $1,080/0.2 mg | $2,120/0.5 mg | $3,145/1 mg | $7,695/5 mg
Activity Data
(VEGFA (Vascular endothelial growth factor A) is a growth factor and can be cleaved into several isoforms, including VEGF121. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It is accepted that the VEGF121 isoform stimulates the proliferation of vein endothelial cells. Thus, proliferation assay of recombinant human VEGF121 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of VEGF121. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of ECV-304 cells after incubation with VEGF121 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant VEGF121 for 48h. The result was shown in Figure 2. It was obvious that VEGF121 significantly increased cell viability of ECV-304 cells.)
Vascular Endothelial Growth Factor 121 (VEGF121), Active Protein (Cat# AAA2097243)
Full Name
Active Vascular Endothelial Growth Factor 121 (VEGF121)
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$220/0.01 mg | $460/0.05 mg | $705/0.1 mg | $875/0.2 mg | $1,705/0.5 mg | $2,540/1 mg | $6,205/5 mg
Sequence Information
N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP), Recombinant Protein (Cat# AAA2090439)
Full Name
Recombinant N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP)
Gene Names
Nppa; ANF; ANP; Pnd; RATANF
Applications
WB
Purity
> 95%
Pricing
$155/0.01 mg | $275/0.05 mg | $415/0.1 mg | $505/0.2 mg | $970/0.5 mg | $1,425/1 mg | $3,490/5 mg | $15,690/5x5 mg
Thioredoxin, Active Protein (Cat# AAA144064)
Full Name
Recombinant Human Thioredoxin His Tag
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing
$2,380/1 mg | $250/0.025 mg | $180/0.005 mg | $10,490/5x1 mg
Activity Data
(TNFRSF7 (Tumor necrosis factor receptor superfamily member 7), also known as CD27 antigen, is a member of the TNF-receptor superfamily. This receptor is thought to be involved in tumor necrosis factor-activated receptor activity by binding with TNFs. Thus, a binding ELISA assay was conducted to detect the association of TNFRSF7 with TNFa. Briefly, recombinant human TNFRSF7 were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF7 with TNFa was shown in Figure 1 and this effect was in a dose dependent manner.)
Tumor Necrosis Factor Receptor Superfamily, Member 7 (TNFRSF7), Active Protein (Cat# AAA2097222)
Full Name
Active Tumor Necrosis Factor Receptor Superfamily, Member 7 (TNFRSF7)
Gene Names
CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$160/0.01 mg | $300/0.05 mg | $460/0.1 mg | $565/0.2 mg | $1,080/0.5 mg | $1,600/1 mg | $3,920/5 mg
Activity Data
(Figure. The binding activity of TNFRSF11A with TRAF5.RANK tumor necrosis factor receptor superfamily member 11A protein (TNFRSF11A) also known as receptor Activator of Nuclear Factor kappa B (RANK) or TRANCE Receptoris a member of the tumor necrosis factor receptor (TNFR) molecular sub-family. TNFRSF11A is the receptor for RANK-Ligand (RANKL) and part of the RANK/RANKL/OPG signaling pathway that regulates osteoclast differentiation and activation. It is associated with bone remodeling and repair, immune cell function, lymph node development, thermal regulation, and mammary gland development. Besides, TNF Receptor Associated Factor 5 (TRAF5) has been identified as an interactor of TNFRSF11A, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TNFRSF11A and recombinant human TRAF5. Briefly, TNFRSF11A were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TRAF5-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF11A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF11A and TRAF5 was shown in Figure 1, and this effect was in a dose dependent manner.)
Receptor Activator Of Nuclear Factor Kappa B (RANk), Active Protein (Cat# AAA2105163)
Full Name
Active Receptor Activator Of Nuclear Factor Kappa B (RANk)
Gene Names
TNFRSF11A; FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$225/0.01 mg | $480/0.05 mg | $740/0.1 mg | $915/0.2 mg | $1,790/0.5 mg | $2,645/1 mg | $6,465/5 mg
SDS-Page
CDA, Active Protein (Cat# AAA203660)
Full Name
CDA, 1-146aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
CDA; CDD
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
$215/0.02 mg | $340/0.1 mg | $765/0.5 mg | $3,195/5x0.5 mg
