SDS-Page
(SDS-PAGE of 78kDa Grp78 (Bip) protein (SPR-107).)
Grp78 (Bip) Active Protein | HSPA5 active protein
Recombinant Human GRP78 (Bip)
Gene Names
HSPA5; BIP; MIF2; GRP78; HEL-S-89n
Applications
Western Blot, ELISA
Purity
Affinity Purified
Synonyms
Grp78 (Bip); Recombinant Human GRP78 (Bip); BIP; Grp78; HspA5; MIF2; immunoglobulin heavy chain binding protein; HSPA5 active protein
Host
Human
Purity/Purification
Affinity Purified
Form/Format
20mM Tris, 50mM KCl, 5mM MgCl2, 1mM DTT, pH7.5
Concentration
4 mg/mL (varies by lot)
Sequence Length
654
Applicable Applications for HSPA5 active protein
ATPase Assay, Western Blot control (WB), Binding Assay, ELISA (EIA) reference standard
Expression System
E Coli
Certificate of Analysis
This product has been certified >90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 2.3uM phosphate liberated/hr/ug protein in a 200ul reaction at 37 degree C (pH 8) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
Conjugate Tag
His tag
Cellular location
Endoplasmic reiculum lumen, Melanosome
Preparation and Storage
Store at -20°C for 1 year + Avoid freeze/ thaw cycles.
SDS-Page
(SDS-PAGE of 78kDa Grp78 (Bip) protein (SPR-107).)
SDS-Page
(SDS-PAGE of 78kDa Grp78 (Bip) protein (SPR-107).)
Related Product Information for HSPA5 active protein
Background Info: GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia (Barnes), to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
Product Categories/Family for HSPA5 active protein
References
1. Cho, S. et al (2007). Mol Cell Biol 27(1): 368-83. 2. Yang, Y. et al. (1998) J Biol Chem 273:25552-25555. 3. Luo, S. et al (2006) 26 (15): 5688-97. 4. Yu, Z. et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi, R. et al. (1999) Anticancer Res. 19: 4333-4336. 6. Laquerre, S. et al. (1998) J. Virology 72: 4940-4949. 7. Dong, D. et al. (2005) Cancer Res 65(13): 5785-91.
1. Zwang, N.A. et al. (2009). Identification of Phosphorylation-Dependent Binding Partners of Aquaporin-2 Using Protein Mass Spectrometry. J. Proteome Res. 8 (3), 1540-1554. doi: 10.1021/pr800894p
2. Speth C. et al. (2013). Neutrophils Turn Plasma Proteins into Weapons against HIV-1. PLoS ONE 8(6): e66073. doi:10.1371/journal.pone.0066073
3. Ciplys, E., Au Ynait, A., Slibinskas, R. (2014). Generation of human ER chaperone BiP in yeast Saccharomyces cerevisiae. Microb Cell Fact. 13(1):22. doi: 10.1186/1475-2859-13-22
1. Zwang, N.A. et al. (2009). Identification of Phosphorylation-Dependent Binding Partners of Aquaporin-2 Using Protein Mass Spectrometry. J. Proteome Res. 8 (3), 1540-1554. doi: 10.1021/pr800894p
2. Speth C. et al. (2013). Neutrophils Turn Plasma Proteins into Weapons against HIV-1. PLoS ONE 8(6): e66073. doi:10.1371/journal.pone.0066073
3. Ciplys, E., Au Ynait, A., Slibinskas, R. (2014). Generation of human ER chaperone BiP in yeast Saccharomyces cerevisiae. Microb Cell Fact. 13(1):22. doi: 10.1186/1475-2859-13-22
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~78 KD
NCBI Official Full Name
78 kDa glucose-regulated protein
NCBI Official Synonym Full Names
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
NCBI Official Symbol
HSPA5
NCBI Official Synonym Symbols
BIP; MIF2; GRP78; HEL-S-89n
NCBI Protein Information
78 kDa glucose-regulated protein; immunoglobulin heavy chain-binding protein; epididymis secretory sperm binding protein Li 89n; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
UniProt Protein Name
78 kDa glucose-regulated protein
UniProt Gene Name
HSPA5
UniProt Synonym Gene Names
GRP78; GRP-78; BiP
UniProt Entry Name
GRP78_HUMAN
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Product Notes
The HSPA5 hspa5 (Catalog #AAA806942) is an Active Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Grp78 (Bip) can be used in a range of immunoassay formats including, but not limited to, ATPase Assay, Western Blot control (WB), Binding Assay, ELISA (EIA) reference standard. Researchers should empirically determine the suitability of the HSPA5 hspa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Grp78 (Bip), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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