Sequence
Interleukin 1 Receptor Antagonist (IL1RA) Active Protein | IL1RA active protein
Active Interleukin 1 Receptor Antagonist (IL1RA)
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence
Sequence
Activity
(IL1RA (interleukin-1 receptor antagonist) is an agent that binds to the cell surface interleukin-1 receptor (IL-1R), which would prevent IL-1 from intracellular signal transduction. Besides, mouse IL1R1 and rat IL1R1 share similarities in amino acid sequence with the identity of 85.6%. Thus, a binding ELISA assay was constructed to detect the association of recombinant mouse IL1RA with recombinant rat IL1R1. Briefly, IL1RA were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1R1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL1RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50AL stop solution to the wells and read at 450nm immediately. The binding activity of IL1RA with IL1R1 was shown in Figure 1 and this effect was in a dose dependent manner.Figure 1. The binding activity of IL1RA with IL1R1.)
Activity
(IL1RA (interleukin-1 receptor antagonist) is an agent that binds to the cell surface interleukin-1 receptor (IL-1R), which would prevent IL-1 from intracellular signal transduction. Besides, mouse IL1R1 and rat IL1R1 share similarities in amino acid sequence with the identity of 85.6%. Thus, a binding ELISA assay was constructed to detect the association of recombinant mouse IL1RA with recombinant rat IL1R1. Briefly, IL1RA were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1R1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL1RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50AL stop solution to the wells and read at 450nm immediately. The binding activity of IL1RA with IL1R1 was shown in Figure 1 and this effect was in a dose dependent manner.Figure 1. The binding activity of IL1RA with IL1R1.)
Identification
(Figure 2. Gene Sequencing (extract))
Identification
(Figure 2. Gene Sequencing (extract))
SDS-PAGE
(Figure 3. SDS-PAGESample: Active recombinant IL1RA, Mouse)
SDS-PAGE
(Figure 3. SDS-PAGESample: Active recombinant IL1RA, Mouse)
Western Blot (WB)
(Figure 4. Western BlotSample: Recombinant IL1RA, Mouse; Antibody: Rabbit Anti-Mouse IL1RA Ab (MBS2002916))
Western Blot (WB)
(Figure 4. Western BlotSample: Recombinant IL1RA, Mouse; Antibody: Rabbit Anti-Mouse IL1RA Ab (MBS2002916))
NCBI and Uniprot Product Information
Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.
Uniprot Description
IL1RN: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. The intracellular form of IL1RN is predominantly expressed in epithelial cells. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine; Secreted; Secreted, signal peptide; Vesicle
Chromosomal Location of Human Ortholog: 2 A3|2 16.36 cM
Cellular Component: cytoplasm; extracellular region; extracellular space; vesicle
Molecular Function: cytokine activity; interleukin-1 receptor antagonist activity; interleukin-1 receptor binding; interleukin-1 Type I receptor antagonist activity; interleukin-1 Type II receptor antagonist activity; interleukin-1, Type I receptor binding; interleukin-1, Type II receptor binding
Biological Process: acute-phase response; cytokine-mediated signaling pathway; fever; immune response; inflammatory response; insulin secretion; lipid metabolic process; memory; negative regulation of apoptosis; negative regulation of cell migration; negative regulation of glutamate secretion; negative regulation of heterotypic cell-cell adhesion; negative regulation of membrane potential; positive regulation of JUN kinase activity; response to glucocorticoid stimulus; sensory perception of pain
Research Articles on IL1RA
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Product Notes
The IL1RA il1rn (Catalog #AAA2097334) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Interleukin 1 Receptor Antagonist (IL1RA) can be used in a range of immunoassay formats including, but not limited to, Cell culture; Activity Assays; In vivo assays. (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the IL1RA il1rn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 1 Receptor Antagonist (IL1RA), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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