LGALS8 active protein

LGALS8, 1-316aa, mouse, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA204201

• Gene Names: Lgals8; Lgals-8; AI326142; D13Ertd524e; 1200015E08Rik
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: LGALS8; 1-316aa; mouse; His tag; E Coli (Bioactivity Validated); Lectin galactose binding soluble 8; Lectin; galactose binding; soluble 8; 1200015E08Rik; AI326142; D13Ertd524e; Lgals-8; LGALS8 active protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMLSLNNL QNIIYNPIIP YVGTITEQLK PGSLIVIRGH VPKDSERFQV DFQLGNSLKP RADVAFHFNP RFKRSSCIVC NTLTQEKWGW EEITYDMPFR KEKSFEIVFM VLKNKFQVAV NGRHVLLYAH RISPEQIDTV GIYGKVNIHS IGFRFSSDLQ SMETSALGLT QINRENIQKP GKLQLSLPFE ARLNASMGPG RTVVIKGEVN TNARSFNVDL VAGKTRDIAL HLNPRLNVKA FVRNSFLQDA WGEEERNITC FPFSSGMYFE MIIYCDVREF KVAINGVHSL EYKHRFKDLS SIDTLSVDGD IRLLDVRSW
• Sequence Length: 316

• Applicable Applications for LGALS8 active protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Biological Activity: The ED50 for this effect is equal or less than 2 ug/ml. Measured by its ability to agglutinate human red blood cells.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for LGALS8 active protein

LGALS8, also known as prostate-specific membrane antigen (PCTA1), is an additional prostate-specific antigen that is overexpressed only in malignant tumors and therefore is a more specific identifier of malignancies. It is a member of the galectin gene family which mediates both cell-cell and cell matrix interactions in a manner similar to the selectin subgroup of C-type lectins. Recombinant mouse LGALS8 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for LGALS8 active protein

Cancer

References

Kobayashi T., et al. (2010) Leukemia. 24 (4):843-50.; Streetly MJ., et al. (2010) Blood. 115 (19):3939-48.

NCBI and Uniprot Product Information

• NCBI GI #: 9256551
• NCBI GeneID: 56048
• NCBI Accession #: NP_061374
• NCBI GenBank Nucleotide #: NM_018886.5
• UniProt Accession #: Q9JL15
• Molecular Weight: 38 KDa (339aa) confirmed by MALDI-TOF
• NCBI Official Full Name: galectin-8 isoform 1
• NCBI Official Synonym Full Names: lectin, galactose binding, soluble 8
• NCBI Official Symbol: Lgals8
• NCBI Official Synonym Symbols: Lgals-8; AI326142; D13Ertd524e; 1200015E08Rik
• NCBI Protein Information: galectin-8
• UniProt Protein Name: Galectin-8
• Protein Family: Galectin
• UniProt Gene Name: Lgals8
• UniProt Synonym Gene Names: Gal-8

Uniprot Description

Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy. Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture. Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52. Required to restrict infection of bacterial invasion such as S.typhimurium. Also required to restrict infection of Picornaviridae viruses. Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.