ACPP active protein

ACPP, 33-386aa, Human, His-tag, Baculovirus (Bioactivity Validated)

Cat. Num. AAA206396

• Gene Names: ACPP; ACP3; 5'-NT; ACP-3
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: ACPP; 33-386aa; Human; His-tag; Baculovirus (Bioactivity Validated); Prostatic acid phosphatase isoform PAP; 5-NT; ACP-3; ACP3; 5-nucleotidase Acid phosphatase prostate; ACP 3; acpP Ecto-5-nucleotidase; PAP; PPAP_HUMAN Prostatic acid phosphatase; Thiamine monophosphatase; TMPase; ACPP active protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: KELKFVTLVF RHGDRSPIDT FPTDPIKESS WPQGFGQLTQ LGMEQHYELG EYIRKRYRKF LNESYKHEQV YIRSTDVDRT LMSAMTNLAA LFPPEGVSIW NPILLWQPIP VHTVPLSEDQ LLYLPFRNCP RFQELESETL KSEEFQKRLH PYKDFIATLG KLSGLHGQDL FGIWSKVYDP LYCESVHNFT LPSWATEDTM TKLRELSELS LLSLYGIHKQ KEKSRLQGGV LVNEILNHMK RATQIPSYKK LIMYSAHDTT VSGLQMALDV YNGLLPPYAS CHLTELYFEK GEYFVEMYYR NETQHEPYPL MLPGCSPSCP LERFAELVGP VIPQDWSTEC MTTNSHQGTE DSTDHHHHHH
• Sequence Length: 386

• Applicable Applications for ACPP active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Specific activity is > 100,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ACPP active protein

ACPP, also known as prostatic acid phosphatase isoform PAP, is a type I integral membrane protein of the plasma membrane and lysosomes, and a secreted form also exists. The concentration of ACPP is elevated in the circulation of prostate cancer patients, making the enzyme a marker for the progression of prostate cancer. Recombinant human ACPP, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for ACPP active protein

Cancer; Enzymes & Proteases

References

Chuang TD., et al. (2010) J Biol Chem. 285:23598-23606.; Taira A., et al. (2007) Oncology. 21:1003-1010.;

NCBI and Uniprot Product Information

• NCBI GI #: 6382064
• NCBI GeneID: 55
• NCBI Accession #: NP_001090
• NCBI GenBank Nucleotide #: NM_001099.4
• UniProt Accession #: P15309; Q5FBY0; Q96KY0; Q96QK9; Q96QM0; D3DNC6
• Molecular Weight: 41.8kDa (360aa) 40-57KDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: prostatic acid phosphatase isoform PAP
• NCBI Official Synonym Full Names: acid phosphatase, prostate
• NCBI Official Symbol: ACPP
• NCBI Official Synonym Symbols: ACP3; 5'-NT; ACP-3
• NCBI Protein Information: prostatic acid phosphatase
• UniProt Protein Name: Prostatic acid phosphatase
• Protein Family: Acyl carrier protein
• UniProt Gene Name: ACPP
• UniProt Synonym Gene Names: PAP; 5'-NT; TMPase

NCBI Description

This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]

Uniprot Description

A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.