GSR active protein

GSR, 43-522aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA204266

• Gene Names: GSR; HEL-75; HEL-S-122m
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: GSR; 43-522aa; Human; His tag; E Coli (Bioactivity Validated); Glutathione reductase; GLuR; GRD1; GSR active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAMACRQ EPQPQGPPPA AGAVASYDYL VIGGGSGGLA SARRAAELGA RAAVVESHKL GGTCVNVGCV PKKVMWNTAV HSEFMHDHAD YGFPSCEGKF NWRVIKEKRD AYVSRLNAIY QNNLTKSHIE IIRGHAAFTS DPKPTIEVSG KKYTAPHILI ATGGMPSTPH ESQIPGASLG ITSDGFFQLE ELPGRSVIVG AGYIAVEMAG ILSALGSKTS LMIRHDKVLR SFDSMISTNC TEELENAGVE VLKFSQVKEV KKTLSGLEVS MVTAVPGRLP VMTMIPDVDC LLWAIGRVPN TKDLSLNKLG IQTDDKGHII VDEFQNTNVK GIYAVGDVCG KALLTPVAIA AGRKLAHRLF EYKEDSKLDY NNIPTVVFSH PPIGTVGLTE DEAIHKYGIE NVKTYSTSFT PMYHAVTKRK TKCVMKMVCA NKEEKVVGIH MQGLGCDEML QGFAVAVKMG ATKADFDNTV AIHPTSSEEL VTLR
• Sequence Length: 522

• Applicable Applications for GSR active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 45 units/mg. The unit definition for glutathione reductase activity may be expressed in terms of the oxidation of NADPH or the reduction of GSSG since their molar ratio is 1:1. One unit of glutathione reductase oxidizes 1 umol of NADPH per minute at 37C, pH 7.5.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GSR active protein

Glutathione reductase, also known as GSR, is a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein and plays a role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+ ->2GSH + NADP+. In most eukaryotic cells, GSR maintains the ratio of [GSH] / [GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis. Recombinant human GSR protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for GSR active protein

Redox Proteins

References

Stoll V S., et al. (1997) Biochemistry. 36:6437-6447.; Karplus P A., et al. (1987) J Mol biol. 195:701-729.

NCBI and Uniprot Product Information

• NCBI GI #: 50301238
• NCBI GeneID: 2936
• NCBI Accession #: NP_000628
• NCBI GenBank Nucleotide #: NM_000637.4
• UniProt Accession #: P00390; Q7Z5C9; Q9NP63; C8KIL8; C8KIL9; C8KIM0; D3DSV3
• Molecular Weight: 54.3 kDa (504aa) confirmed by MALDI-TOF
• NCBI Official Full Name: glutathione reductase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: glutathione-disulfide reductase
• NCBI Official Symbol: GSR
• NCBI Official Synonym Symbols: HEL-75; HEL-S-122m
• NCBI Protein Information: glutathione reductase, mitochondrial
• UniProt Protein Name: Glutathione reductase, mitochondrial
• Protein Family: Glutathione reductase
• UniProt Gene Name: GSR
• UniProt Synonym Gene Names: GLUR; GRD1; GR; GRase

NCBI Description

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2010]

Uniprot Description

Maintains high levels of reduced glutathione in the cytosol.