Sequence Information
N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP) Recombinant Protein | NT-ProANP recombinant protein
Recombinant N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP)
Gene Names
Nppa; ANF; ANP; Pnd; RATANF
Applications
SDS-Page, Western Blot
Purity
> 95%
Synonyms
N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP); Recombinant N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP); ANH; ANF; PND; NPPA; CDD-ANF; Atrial Natriuretic Factor; Atrial Natriuretic Hormone; Atriopeptin; Natriuretic Peptide Precursor A; Prepronatriodilatin; Cardiodilatin-related peptide; NT-ProANP recombinant protein
Host
E.coli
Purity/Purification
> 95%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 200ug/mL (varies by lot)
Applicable Applications for NT-ProANP recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB)
Organism Species
Rattus norvegicus (Rat)
Source
Prokaryotic expression
Residues
Asn25~Arg122 linked with LEHHHHHH
Tags
N-terminal His Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
5.2
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence Information
Sequence Information
Testing Data
Testing Data
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 12.9kDa
Accurate Molecular Mass: 17kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 17kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
Natriuretic peptides A
NCBI Official Synonym Full Names
natriuretic peptide A
NCBI Official Symbol
Nppa
NCBI Official Synonym Symbols
ANF; ANP; Pnd; RATANF
NCBI Protein Information
natriuretic peptides A
UniProt Protein Name
Natriuretic peptides A
UniProt Gene Name
Nppa
UniProt Synonym Gene Names
ANF; ANP
NCBI Description
peptide involved in the control of fluid volume and vascular function [RGD, Feb 2006]
Uniprot Description
NPPA: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Defects in NPPA are the cause of familial atrial fibrillation type 6 (ATFB6). Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity, progressive deterioration of atrial electromechanical function and ineffective pumping of blood into the ventricles. It can be associated with palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the natriuretic peptide family.
Protein type: Hormone; Secreted; Secreted, signal peptide
Research Articles on NT-ProANP
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Product Notes
The NT-ProANP nppa (Catalog #AAA2090439) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). Researchers should empirically determine the suitability of the NT-ProANP nppa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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