AXL recombinant protein

AXL Protein, Mouse, Recombinant (His & hFc Tag)

Cat. Num. AAA8122548

• Gene Names: Axl; Ark; Ufo; Tyro7; AI323647
• Purity: >90% as determined by SDS-PAGE
• Synonyms: AXL; AXL Protein; Mouse; Recombinant (His & hFc Tag); Mouse Axl Kinase Protein (His & Fc Tag); AI323647 Protein; Ark Protein; Tyro7 Protein; Ufo Protein; AXL receptor tyrosine kinase; AXL recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro443

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His 20
• Tag: C-human IgG1-Fc & His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Pro 443) of mouse AXL (NP_033491.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for AXL recombinant protein

Background: Axl receptor tyrosine kinase, together with Tyro3 and Mer, constitute the TAM family of receptor tyrosine kinases. In the nervous system, Axl and its ligand Growth-arrest-specific protein 6 (Gas6) are expressed on multiple cell types. Axl functions in dampening the immune response, regulating cytokine secretion, clearing apoptotic cells and debris, and maintaining cell survival. Axl is upregulated in various disease states, such as in the cuprizone toxicity-induced model of demyelination and in multiple sclerosis (MS) lesions, suggesting that it plays a role in disease pathogenesis. Axl expression correlates with poor prognosis in several cancers. Axl mediates multiple oncogenic phenotypes and activation of these RTKs constitutes a mechanism of chemoresistance in a variety of solid tumors. Axl contributes to cell survival, migration, invasion, metastasis and chemosensitivity justify further investigation of Axl as novel therapeutic targets in cancer. The receptor tyrosine kinase AXL is thought to play a role in metastasis. The soluble AXL receptor as a therapeutic candidate agent for treatment of metastatic ovarian cancer. GAS6/AXL targeting as an effective strategy for inhibition of metastatic tumor progression in vivo.

Product Categories/Family for AXL recombinant protein

Receptor Tyrosine Kinases (RTKs)

References

Weinger JG, et al. (2011) Loss of the receptor tyrosine kinase Axl leads to enhanced inflammation in the CNS and delayed removal of myelin debris during Experimental Autoimmune Encephalomyelitis. J Neuroinflammation. 8: 49.Linger RM, et al. (2010) Taking aim at Mer and Axl receptor tyrosine kinases as novel therapeutic targets in solid tumors. Expert Opin Ther Targets. 14(10): 1073-90.Cavet ME, et al. (2010) Gas6-Axl pathway: the role of redox-dependent association of Axl with nonmuscle myosin IIB. Hypertension. 56(1): 105-11.Rankin EB, et al. (2010) AXL is an essential factor and therapeutic target for metastatic ovarian cancer. Cancer Res. 70(19): 7570-9.

NCBI and Uniprot Product Information

• NCBI GI #: 31542164
• NCBI GeneID: 26362
• NCBI Accession #: NP_033491.2
• NCBI GenBank Nucleotide #: NM_009465.4
• UniProt Accession #: Q00993; Q80YQ3
• Molecular Weight: 98,191 Da
• NCBI Official Full Name: tyrosine-protein kinase receptor UFO isoform 1
• NCBI Official Synonym Full Names: AXL receptor tyrosine kinase
• NCBI Official Symbol: Axl
• NCBI Official Synonym Symbols: Ark; Ufo; Tyro7; AI323647
• NCBI Protein Information: tyrosine-protein kinase receptor UFO; ufo oncogene homolog; adhesion-related kinase
• UniProt Protein Name: Tyrosine-protein kinase receptor UFO
• Protein Family: Tyrosine-protein kinase receptor
• UniProt Gene Name: Axl
• UniProt Synonym Gene Names: Ark; Ufo
• UniProt Entry Name: UFO_MOUSE

Uniprot Description

AXL: a receptor tyrosine kinase that may function as a signal transducer between specific cell types of mesodermal origin. Interacts with SKP1. Overexpression in tissue culture causes oncogenic transformation. Overexpressed in several cancers including thyroid, ovarian, gastric, ER+ breast cancer and acute myeloid leukemia, where it is associated with poor prognosis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Oncoprotein; Kinase, protein; TK group; Axl family

Cellular Component: extracellular space; cell surface; membrane; cell; integral to membrane; plasma membrane; intracellular

Molecular Function: transferase activity; phosphatidylserine binding; protein heterodimerization activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; phosphoinositide 3-kinase binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; myosin heavy chain binding; protein kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; neuron migration; protein amino acid phosphorylation; enzyme linked receptor protein signaling pathway; positive regulation of natural killer cell differentiation; erythrocyte homeostasis; ovulation cycle; natural killer cell differentiation; negative regulation of interferon-gamma production; protein kinase B signaling cascade; negative regulation of neuron apoptosis; inflammatory response; cell differentiation; secretion by cell; forebrain cell migration; negative regulation of lymphocyte activation; platelet activation; immune system process; vagina development; negative regulation of tumor necrosis factor production; phagocytosis; positive regulation of protein kinase B signaling cascade; cellular response to extracellular stimulus; innate immune response; spermatogenesis; blood vessel remodeling; positive regulation of cytokine and chemokine mediated signaling pathway; phosphorylation; apoptotic cell clearance; negative regulation of apoptosis