SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
GOT2 active protein
Recombinant GOT2
Gene Names
Got2; Got-2; Kyat4; mAspAT; FABP-pm; AL022787
Applications
SDS-Page
Purity
>90% by SDS-PAGE
Synonyms
GOT2; Recombinant GOT2; AATM_HUMAN; AL022787; Aspartate aminotransferase; Aspartate aminotransferase 2; mitochondrial; Aspartate transaminase 2; ASPATA; EC 2.6.1.1; FABP 1; FABP pm; FABP-1; FABPpm; Fatty acid binding protein; Fatty acid-binding protein; FLJ40994; Glutamate oxaloacetate transaminase 2; Glutamate oxaloacetate transaminase; Glutamic oxaloacetic transaminase 2; mitochondrial (aspartate aminotransferase 2); Got 2; KAT4; KATIV; kynurenine aminotransferase 4; Kynurenine aminotransferase IV; kynurenine-oxoglutarate transaminase 4; kynurenine-oxoglutarate transaminase IV; mAspAT; MGC102129; MGC115763; mitAAT; Mitochondrial aspartate aminotransferase; OTTMUSP00000017748; Plasma membrane fatty acid binding protein; Plasma membrane-associated fatty acid-binding protein; Transaminase A; GOT2 active protein
Host
E Coli
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
Concentration
0.5mg/ml (determined by Bradford assay) (varies by lot)
Sequence Positions
30-430aa
Sequence
MGSSHHHHHH SSGLVPRGSH M SSWWTHVEM GPPDPILGVT EAFKRDTNSK KMNLGVGAYR DDNGKPYVLP SVRKAEAQIA AKNLDKEYLP IGGLAEFCKA SAELALGENN EVLKSGRFVT VQTISGTGAL RVGASFLQRF FKFSRDVFLP KPSWGNHTPI FRDAGMQLQG YRYYDPKTCG FDFSGALEDI SKIPEQSVLL LHACAHNPTG VDPRPEQWKE IASVVKKKNL FAFFDMAYQG FASGDGDKDA WAVRHFIEQG INVCLCQSYA KNMGLYGERV GAFTVVCKDA EEAKRVESQL KILIRPLYSN PPLNGARIAA TILTSPDLRK QWLQEVKGMA DRIISMRTQL VSNLKKEGSS HNWQHITDQI GMFCFTGLKP EQVERLTKEF SVYMTKDGRI SVAGVTSGNV GYLAHAIHQV TK
Sequence Length
430
Applicable Applications for GOT2 active protein
SDS-PAGE, Enzyme Activity
Species
Mouse
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Biological Activity
Specific activity is > 20 units/mg, and is defined as the amount of enzyme that converts 1umole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for GOT2 active protein
GOT2, also known aspartate aminotransferase, mitochondrial, belongs to the class-I
Pyridoxal-phosphate-dependent aminotransferase family. Glutamate oxaloacetate transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant mouse GOT2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for GOT2 active protein
References
Honorat JA., et al. (2017) PLoS One. 12(11):e0187215. Han Q., et al. (2010) BMC Biochemistry. 11:19.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46.8 kDa (422aa), confirmed by MALDI-TOF
NCBI Official Full Name
aspartate aminotransferase, mitochondrial
NCBI Official Synonym Full Names
glutamatic-oxaloacetic transaminase 2, mitochondrial
NCBI Official Symbol
Got2
NCBI Official Synonym Symbols
Got-2; Kyat4; mAspAT; FABP-pm; AL022787
NCBI Protein Information
aspartate aminotransferase, mitochondrial
UniProt Protein Name
Aspartate aminotransferase, mitochondrial
UniProt Gene Name
Got2
UniProt Synonym Gene Names
Got-2; mAspAT; FABP-1; FABPpm
Uniprot Description
Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.
Research Articles on GOT2
Similar Products
Product Notes
The GOT2 got2 (Catalog #AAA201147) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 30-430aa. AAA Biotech's GOT2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Enzyme Activity. Researchers should empirically determine the suitability of the GOT2 got2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH M SSWWTHVEM GPPDPILGVT EAFKRDTNSK KMNLGVGAYR DDNGKPYVLP SVRKAEAQIA AKNLDKEYLP IGGLAEFCKA SAELALGENN EVLKSGRFVT VQTISGTGAL RVGASFLQRF FKFSRDVFLP KPSWGNHTPI FRDAGMQLQG YRYYDPKTCG FDFSGALEDI SKIPEQSVLL LHACAHNPTG VDPRPEQWKE IASVVKKKNL FAFFDMAYQG FASGDGDKDA WAVRHFIEQG INVCLCQSYA KNMGLYGERV GAFTVVCKDA EEAKRVESQL KILIRPLYSN PPLNGARIAA TILTSPDLRK QWLQEVKGMA DRIISMRTQL VSNLKKEGSS HNWQHITDQI GMFCFTGLKP EQVERLTKEF SVYMTKDGRI SVAGVTSGNV GYLAHAIHQV TK. It is sometimes possible for the material contained within the vial of "GOT2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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