Thioredoxin Active Protein | TXN1 active protein

Recombinant Human Thioredoxin His Tag

Cat. Num. AAA144064

• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Thioredoxin; Recombinant Human Thioredoxin His Tag; TXN1 Human; His; Thioredoxin Human Recombinant; His Tag; ATL-derived factor; ADF; Surface-associated sulphydryl protein; SASP; TXN; TRDX; TRX; TRX1; MGC61975; DKFZp686B1993; TXN1; TXN1 active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: TXN1 solution containing 1x PBS pH 7.4.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV
• Sequence Length: 218

• Biological Activity: Specific activity is 7-10 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for TXN1 active protein

Description: Thioredoxin Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids (1-105 a.a.) and having a molecular mass of 13.9 kDa (Molecular weight on SDS-PAGE will appear higher). TXN protein is fused to a 20 amino acid His-Tag at N-terminus and purified by standard chromatography.

Introduction: Thioredoxins are small disulphide-containing redox proteins (within the conserved Cys-Gly-Pro-Cys active site) that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation.

Product Categories/Family for TXN1 active protein

Recombinant & Natural Proteins; Recombinant Proteins; Thioredoxin

NCBI and Uniprot Product Information

• NCBI GI #: 5454028
• NCBI GeneID: 6237
• NCBI Accession #: NP_006261.1
• NCBI GenBank Nucleotide #: NM_006270.3
• UniProt Accession #: P10301; Q6FH12
• Molecular Weight: 23,480 Da
• NCBI Official Full Name: ras-related protein R-Ras
• NCBI Official Synonym Full Names: related RAS viral (r-ras) oncogene homolog
• NCBI Official Symbol: RRAS
• NCBI Protein Information: ras-related protein R-Ras; Oncogene RRAS; p23
• UniProt Protein Name: Ras-related protein R-Ras
• Protein Family: Thioredoxin
• UniProt Gene Name: RRAS
• UniProt Entry Name: RRAS_HUMAN

NCBI Description

The protein encoded by this gene is a small GTPase involved in diverse processes including angiogenesis, vascular homeostasis and regeneration, cell adhesion, and neuronal axon guidance. Mutations in this gene are found in many invasive cancers. [provided by RefSeq, Jul 2015]

Uniprot Description

RRas: a small G protein of the Ras family. Promotes focal adhesion formation by signaling to FAK and p130(Cas). Promotes tumor growth of cervical epithelial cells and increases their migration potential over collagen through a pathway that involves PI 3-K.

Protein type: G protein, monomeric, Ras; Motility/polarity/chemotaxis; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: focal adhesion; plasma membrane

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; protein complex binding

Biological Process: axon guidance; synaptic transmission; positive regulation of angiogenesis; metabolic process; leukocyte differentiation; Ras protein signal transduction; regulation of protein kinase B signaling cascade; negative regulation of cell migration