SDS-Page
CDA active protein
CDA, 1-146aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
CDA; CDD
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
CDA; 1-146aa; Human; His tag; E Coli (Bioactivity Validated); Cytidine deaminase; CDD; Cytidine aminohydrolase; Cytosine nucleoside deaminase; CDA active protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1 mM DTT, 2 mM EDTA, 100 mM NaCl, 40% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ
Sequence Length
146
Applicable Applications for CDA active protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Biological Activity
Specific activity is > 10,000 pmol/min/ug, and is defined as the amount of required to deaminate 1.0 pmole of cytidine per min at pH 7.5 at 25C.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for CDA active protein
CDA (Cytidine deaminase) is an enzyme that scavenges exogenous and endogenous cytidine and 2-deoxycytidine for uMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. The protein also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. It can form homotetramers and is mainly expressed in granulocytes. Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for CDA active protein
References
Evrard A., et al. (2010) Ther Drug Monit. 32 (1):53-60.; Seeberg EC., et a/. (1998) Blood. 91 (11):4127-35.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
18.3 kDa (166aa), confirmed by MALDI-TOF
NCBI Official Full Name
Cytidine deaminase
NCBI Official Synonym Full Names
cytidine deaminase
NCBI Official Symbol
CDA
NCBI Official Synonym Symbols
CDD
NCBI Protein Information
cytidine deaminase
UniProt Protein Name
Cytidine deaminase
Protein Family
UniProt Gene Name
CDA
UniProt Synonym Gene Names
CDD
NCBI Description
This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias. [provided by RefSeq, Jul 2008]
Uniprot Description
This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.
Research Articles on CDA
Similar Products
Product Notes
The CDA cda (Catalog #AAA203660) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's CDA can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the CDA cda for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ. It is sometimes possible for the material contained within the vial of "CDA, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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