Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
1205 results for "Rat IgG Isotype Control" - showing 1-50
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-TWIST monoclonal antibody. )
TWIST, Monoclonal Antibody (Cat# AAA120163)
Full Name
Mouse monoclonal antibody Anti-Human TWIST
Gene Names
TWIST1; CRS; CSO; SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38
Reactivity
Human
Applications
DB, ICC, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-ACTB monoclonal antibody. )
ACTB, Monoclonal Antibody (Cat# AAA120059)
Full Name
Mouse monoclonal antibody Anti-Human ACTB
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human
Applications
DB, ICC, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Western Blot (WB)
(Detection of Human ADAMTS15 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2ug/ml of Human ADAMTS15 Monoclonal Antibody followed by HRP- conjugated Mouse IgG Secondary Antibody. A specific band was detected for mature ADAMTS15 at approximately 75kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
ADAMTS15, Monoclonal Antibody (Cat# AAA6001180)
Full Name
ADAMTS15 (A Disintegrin And Metalloproteinase With Thrombospondin Motifs 15, ADAM-TS 15, ADAM-TS15, ADAMTS-15)
Reactivity
Human
Applications
WB, IHC, FC/FACS
Purity
Affinity Purified
Purified by Protein A/G affinity chromatography.
Purified by Protein A/G affinity chromatography.
Pricing
$735/0.1 mg | $3,290/5x0.1 mg
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-DNMT3B monoclonal antibody. )
DNMT3B, Monoclonal Antibody (Cat# AAA120171)
Full Name
Mouse monoclonal antibody Anti-Human DNMT3B
Gene Names
DNMT3B; ICF; ICF1; M.HsaIIIB
Reactivity
Human
Applications
DB, ICC, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-NUDT21 monoclonal antibody. )
NUDT21, Monoclonal Antibody (Cat# AAA120191)
Full Name
Mouse monoclonal antibody Anti-Human NUDT21
Gene Names
NUDT21; CPSF5; CFIM25
Reactivity
Human
Applications
DB, ICC, IP, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-ABCF2 monoclonal antibody. )
ABCF2, Monoclonal Antibody (Cat# AAA120190)
Full Name
Mouse monoclonal antibody Anti-Human ABCF2
Gene Names
ABCF2; ABC28; HUSSY18; HUSSY-18; EST133090
Reactivity
Human
Applications
DB, IP, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-BRPF1 monoclonal antibody. )
BRPF1, Monoclonal Antibody (Cat# AAA120114)
Full Name
Mouse monoclonal antibody Anti-Human BRPF1
Gene Names
BRPF1; BR140
Reactivity
Human
Applications
DB, IP, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation of PD-L1 in HeLa CellsLoading: 15ug of lysates per lane. Antibodies: MBS151123 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
PDL-1, Polyclonal Antibody (Cat# AAA151123)
Full Name
PDL-1 Antibody
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC-P, IF, FC
Purity
PD-L1 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescent staining of paraformaldehyde-fixed HeLa cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-Mouse IgG-CF488 (Green). Counter stained with Phalloidin (red).)
Human Nuclear Antigen, Monoclonal Antibody (Cat# AAA438074)
Full Name
Human Nuclear Antigen
Reactivity
Human and Non-human primates. Does not react with mouse, rat, and chicken. Others not known.
Applications
FC/FACS, IF, IP, ICC, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
(Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA214794)
Full Name
RAT ANTI MOUSE CD8
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Reactivity
Mouse
Applications
FC, IF
Pricing
$195/0.025 mg | $730/5x0.025 mg
Testing Data
(Published customer image: Chronic effects of celastrol on microgliosis in Tg PS1/APPsw mice. A) Representative photomicrographs (taken with a 20x and 60x objective providing a respective magnification of 200x and 600x respectively) depicting the presence of CD45 reactive microglia around Abeta deposits in Tg PS1/APPsw treated with a placebo and celastrol. B) Histogram representing the burden of activated microglia (CD45 positive) in the cortex of Tg PS1/APPsw mice treated with placebo and celastrol pellets. Statistically significant difference in microgliosis burden (P < 0.03) was observed between placebo and celastrol treated mice. (* P < 0.05)..)
CD45, Monoclonal Antibody (Cat# AAA211182)
Full Name
RAT ANTI MOUSE CD45
Gene Names
Ptprc; loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
Applications
IHC-Fr, FC/FACS, IF, IP
Pricing
$475/0.25 mg | $1,995/5x0.25 mg
Western Blot (WB)
(Figure 1. Western blot analysis of ABCB4 using anti-ABCB4 antibody (MBS177897).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: MCF-7 Whole Cell Lysate,Lane 2: SW620 Whole Cell Lysate,Lane 3: 22RV1 Whole Cell Lysate,Lane 4: SKOV Whole Cell Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ABCB4 at approximately 142KD. The expected band size for ABCB4 is at 142KD. )
ABCB4, Polyclonal Antibody (Cat# AAA177897)
Full Name
Anti-ABCB4 Antibody
Gene Names
ABCB4; GBD1; ICP3; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF/ICC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-ARNTL2 monoclonal antibody. )
ARNTL2, Monoclonal Antibody (Cat# AAA120053)
Full Name
Mouse monoclonal antibody Anti-Human ARNTL2
Gene Names
ARNTL2; CLIF; MOP9; BMAL2; PASD9; bHLHe6
Reactivity
Human
Applications
DB, ICC, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-HARS monoclonal antibody. )
HARS, Monoclonal Antibody (Cat# AAA120067)
Full Name
Mouse monoclonal antibody Anti-Human HARS
Gene Names
HARS; HRS; USH3B
Reactivity
Human
Applications
DB, ICC, IP, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-STAT3 monoclonal antibody. )
STAT3, Monoclonal Antibody (Cat# AAA120037)
Full Name
Mouse monoclonal antibody Anti-Human STAT3
Gene Names
STAT3; APRF; HIES; ADMIO
Reactivity
Human
Applications
EIA, ICC, IP, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Testing Data
(Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA212026)
Full Name
RAT ANTI MOUSE CD8
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Applications
FC/FACS, IF
Pricing
$475/0.25 mg | $1,995/5x0.25 mg
Western Blot (WB)
(Western Blot of A431 Cell Lysate using Nucleolin Mouse Monoclonal Antibody (NCL/902))
Nucleolin, Monoclonal Antibody (Cat# AAA438674)
Full Name
Nucleolin (Marker of Human Cells) Mouse Monoclonal Antibody
Gene Names
NCL; C23
Reactivity
Human.
Does not react with Mouse, Rat and Cow
Does not react with Mouse, Rat and Cow
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot
(Anti-WT1 Antibody (Center E361) at 1:1000 dilution + Mouse testis lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 49 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WT1, Polyclonal Antibody (Cat# AAA9203569)
Full Name
WT1 Antibody (Center E361)
Gene Names
WT1; GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
Reactivity
Human, Mouse
Predicted Reactivity: Xenopus, Pig, Rat
Predicted Reactivity: Xenopus, Pig, Rat
Applications
IF, EIA, WB, FC/FACS, IHC
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western Blot Analysis Raji Cell Lysate, Vimentin Mouse Monoclonal Antibody (VM452))
Vimentin, Monoclonal Antibody (Cat# AAA438228)
Full Name
Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody
Gene Names
VIM; HEL113; CTRCT30
Reactivity
Human, Cow, Dog, Cat, Pig, Goat, Chicken.
Does not react with Mouse and Rat
Does not react with Mouse and Rat
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western BlotPositive WB detected in: Hela whole cell lysate, HEK293 whole cell lysateAll lanes: PODXL antibody at 2.5 ug/mlSecondaryGoat polyclonal to Mouse IgG at 1/5000 dilutionPredicted band size: 59, 56 kDaObserved band size: 59 kDa)
Podocalyxin, Monoclonal Antibody (Cat# AAA1498612)
Full Name
Podocalyxin; GCTM-2 antigen; Gp200; PC; PODXL; PCLP; PCLP1
Gene Names
PODXL; PC; PCLP; Gp200; PCLP-1
Reactivity
Human. Other species are not tested. Please decide the specificity by homology
Applications
EIA, IHC, IF, WB, IP, FC/FACS
Purity
>95%, Protein G Purified
Pricing
$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL
Flow Cytometry (FC/FACS)
(Blocking of Fc-receptors by anti-CD16/32 antibody. Ex vivo murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate, left and middle panel) as well as bone marrow-derived macrophages (BMDMs, right panel) were stained with a murine anti-human antibody (fluorescently labelled (AF647) human-specific anti-CD16) with or without pre-incubation with anti-mouse CD16/CD32 (Fc-receptors) antibody (clone 2.4G2). Pre-incubation of cells with anti-CD16/CD32 reduced non-specific binding of the fluorescently labelled antibody to all cell types analysed and shows that this anti-CD16/CD32 antibody acts as an Fc-blocking reagent.)
CD16/CD32, Recombinant Antibody (Cat# AAA488078)
Full Name
Anti-CD16/CD32 [2.4G2]
Gene Names
Fcgr3; CD16
Reactivity
Mouse
Applications
FC/FACS, IF, IP, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-PRPF8 monoclonal antibody. )
PRPF8, Monoclonal Antibody (Cat# AAA120210)
Full Name
Mouse monoclonal antibody Anti-Human PRPF8
Gene Names
PRPF8; PRP8; RP13; HPRP8; PRPC8; SNRNP220
Reactivity
Human
Applications
DB, ICC, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
IgG2b, Isotype Control (Cat# AAA224983)
Full Name
RAT IgG2b NEGATIVE CONTROL
Reactivity
Negative Control
Applications
FC/FACS
Pricing
$380/1 mg | $1,565/5x1 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using anti-PD-1 antibody RMP1-14 Staining of murine splenocytes (unstimulated and 3-day Con A stimulated) with the rabbit-chimeric version of RMP1-14 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody. Con A stimulation induces PD-1 expression as evident by the increased fluorescence signal.)
PD-1, Monoclonal Recombinant Antibody (Cat# AAA488363)
Full Name
Anti-PD-1 [RMP1-14]
Reactivity
Mouse
Applications
BL, FC/FACS, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of NADPH oxidase 4/NOX4 using anti-NADPH oxidase 4/NOX4 antibody Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human HK-2 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human SW579 whole cell lysates, Lane 7: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NADPH oxidase 4/NOX4 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NADPH OXIDASE 4/NOX4 at approximately 67KD. The expected band size for NADPH OXIDASE 4/NOX4 is at 67KD.)
NADPH oxidase 4, Polyclonal Antibody (Cat# AAA176126)
Full Name
Anti-NADPH oxidase 4 antibody
Gene Names
Nox4; AI648021
Reactivity
Human, Rat; Predicted to work with: Mouse
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of SLC10A1 using anti- SLC10A1 antibody (MBS177905).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: mouse liver tissue lysates,After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- SLC10A1 antigen affinity purified polyclonal antibody (MBS177905) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit ( MBS176460 ) with Tanon 5200 system. A specific band was detected for SLC10A1 at approximately 50KD. The expected band size for SLC10A1 is at 38KD.)
SLC10A1, Polyclonal Antibody (Cat# AAA177905)
Full Name
Anti-SLC10A1 Antibody
Gene Names
Slc10a1; Ntcp
Reactivity
Mouse, Rat
Applications
FC/FACS, IF, IHC-P, IHC-F, ICC, WB
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Esophageal Tumor stained with CD86 Monoclonal Antibody (BU63).)
CD86, Monoclonal Antibody (Cat# AAA438121)
Full Name
CD86 (Dendritic Cells Maturation Marker) Mouse Monoclonal Antibody
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, WB, IP, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot detection of COX IV in Mouse skeletal muscel and Jurkat lysates using COX IV mouse mAb (1:1000 diluted). Predicted band size: 17KDa.Observed band size: 17KDa.)
COX IV, Monoclonal Antibody (Cat# AAA475127)
Full Name
Anti-COX IV Mouse mAb
Gene Names
COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1
Reactivity
Human, Mouse, Rat, Hamster, Goat, Monkey
Applications
WB, FC/FACS, ICC, IP, IHC
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of CYP1A1 using anti-CYP1A1 antibody (MBS178240).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Lung Tissue LysateLane 2: Mouse Lung Tissue LysateLane 3: Human Placenta Tissue LysateLane 4: JURKAT Whole Cell LysateAfter Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP1A1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYP1A1 at approximately 58KD. The expected band size for CYP1A1 is at 58KD. )
CYP1A1, Polyclonal Antibody (Cat# AAA178240)
Full Name
Anti-CYP1A1 Antibody
Gene Names
CYP1A1; AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IHC-F, ICC, FC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western Blot detection of human FOLR1 using MBS605669. Lysates were prepared from Hela cells in non-reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with MBS605669 (2ug/ml) and chemiluminescent detection substrate.)
FOLR1, Monoclonal Antibody (Cat# AAA605669)
Full Name
FOLR1 (Folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FOLR, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18)
Gene Names
FOLR1; FBP; FOLR
Reactivity
Human
Applications
Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Pricing
$850/0.1 mg | $3,815/5x0.1 mg
Western Blot (WB)
(Western BlotPositive WB detected in: Mouse brain tissue, Rat brain tissueAll lanes: GFAP antibody at 2.7ug/mlPredicted band size: 50, 51 kDaObserved band size: 50 kDa)
GFAP, Monoclonal Antibody (Cat# AAA7110751)
Full Name
GFAP Monoclonal Antibody
Gene Names
GFAP; ALXDRD
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
>95%, Protein G purified
Pricing
$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of Cannabinoid Receptor I using anti-Cannabinoid Receptor I antibody (MBS1750613).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human 22RV1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cannabinoid Receptor I antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cannabinoid Receptor I at approximately 60KD. The expected band size for Cannabinoid Receptor I is at 53KD. )
Cannabinoid Receptor I, Polyclonal Antibody (Cat# AAA1750613)
Full Name
Anti-Cannabinoid Receptor I antibody
Gene Names
CNR1; CB1; CNR; CB-R; CB1A; CB1R; CANN6; CB1K5
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IHC-P/Fr, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68 Monoclonal Antibody (C68/684).)
CD68, Antibody (Cat# AAA438131)
Full Name
CD68 (Macrophage Marker) Mouse Monoclonal Antibody
Gene Names
CD68; GP110; LAMP4; SCARD1
Reactivity
Human, Monkey, Mouse, and Rat. Others not known.
Applications
WB, IF, FC, Formalin-fixed
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-PDL1 antibody YDC 127.1.1 Mouse lymphocytes were stained with an isotype control or the rabbit-chimeric version of YDC 127.1.1 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
PDL1, Monoclonal Recombinant Antibody (Cat# AAA488305)
Full Name
Anti-PDL1 [YDC 127.1.1]
Gene Names
Cd274; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
Reactivity
Mouse
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Figure A. RPE conjugated Rat anti Mouse CD11b and Pacific Blue conjugated Rat IgG2b isotype control . Figure B. RPE conjugated Rat anti Mouse CD11b and Pacific Blue conjugated Rat anti Mouse GR-1 . All experiments performed on red cell lysed mouse bone marrow gated on mononuclear cells in the presence of Mouse Seroblock (BUF041A). Data acquired on the ZE5 cell analyzer.)
Gr-1, Monoclonal Antibody (Cat# AAA225657)
Full Name
Rat Anti Mouse Gr-1: FITC
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Immunohistochemistry
(MBS9217260 staining beta Tubulin in mouse thymus tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
beta Tubulin, Polyclonal Antibody (Cat# AAA9217260)
Full Name
beta Tubulin
Gene Names
Tubb5; AA408537; AI596182; M(beta)5; B130022C14Rik
Reactivity
Mouse, Rat; Predicted Reactivity: Bovine, Chicken, Human, Mouse, Pig, Rat
Applications
WB, EIA, FC, IHC-P
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Immunofluorescence (IF)
(Immunofluorescent analysis of Human pancreas tissues and Human kidney tissues, using TTR Antibody (C-term). MBS9200982 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).)
TTR, Polyclonal Antibody (Cat# AAA9200982)
Full Name
TTR Antibody (C-term)
Gene Names
TTR; CTS; CTS1; PALB; TBPA; HEL111; HsT2651
Reactivity
Human, Rat
Applications
WB, EIA, IF, IHC-P, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western BlotPositive WB detected in: HepG2 whole cell lysateAll lanes: OCT4 antibody at 1:500SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 39, 31 kDaObserved band size: 39 kDa)
OCT4, Monoclonal Antibody (Cat# AAA7112722)
Full Name
OCT4 Monoclonal Antibody
Gene Names
POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
>95%
Protein G Purified
Protein G Purified
Pricing
$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Mouse Monoclonal Antibody (KRT14/2375).)
Cytokeratin 14 (KRT14), Monoclonal Antibody (Cat# AAA4381054)
Full Name
Cytokeratin 14 (KRT14) (Squamous Cell Marker)
Gene Names
KRT14; K14; NFJ; CK14; EBS3; EBS4
Reactivity
Human, Mouse, Rat, Pig
Applications
FC/FACS, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Figure 1. Western blot analysis of CD163 using anti-CD163 antibody (MBS1750488).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human placenta tissue lysates,Lane 3: mouse NIH3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD163 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD163 at approximately 150KD. The expected band size for CD163 is at 128KD. )
CD163, Antibody (Cat# AAA1750488)
Full Name
Anti-CD163 Picoband antibody
Gene Names
CD163; M130; MM130; SCARI1
Reactivity
Human, Mouse, Rat
Applications
WB, Paraffin-embedded Section, IHC-P, Frozen Section, IHC-Fr, ICC, FC, EIA
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Monoclonal Antibody (9G10.F8.2).)
GRP94 / HSP90B1, Monoclonal Antibody (Cat# AAA439463)
Full Name
GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Rat Monoclonal Antibody
Gene Names
HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
Reactivity
Human, Monkey, Horse, Cow, Sheep, Pig, Rabbit, Dog, Rat, Mouse, Hamster, Guinea Pig, Chicken, Xenopus laevis
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)
Western Blot (WB)
(Figure 1. Western blot analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (MBS175443). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Kidney Tissue Lysate Lane 2: Rat Lung Tissue Lysate Lane 3: Mouse Kidney Tissue Lysate Lane 4: MM453 Whole Cell Lysate Lane 5: SMMC-7721 Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Aquaporin 3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Aquaporin 3 at approximately 32KD. The expected band size for Aquaporin 3 is at 32KD. )
Aquaporin 3, Polyclonal Antibody (Cat# AAA175443)
Full Name
Anti-Aquaporin 3 antibody
Gene Names
AQP3; GIL; AQP-3
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of ADA using anti-ADA antibody (MBS1752027). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U-937 whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ADA antigen affinity purified monoclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.)
ADA, Monoclonal Antibody (Cat# AAA1752027)
Full Name
Anti-ADA Antibody (monoclonal, 6D4)
Reactivity
Human
Applications
WB, IHC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Figure 1. IHC analysis of Calbindin using anti-Calbindin antibody (MBS176994). Calbindin was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (MBS176994) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Calbindin, Polyclonal Antibody (Cat# AAA176994)
Full Name
Anti-Calbindin Antibody
Gene Names
CALB1; CALB
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Figure A. RPE conjugated Rat anti Mouse CD45R and FITC conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD45R and FITC conjugated Rat anti Mouse CD169 . All experiments performed on murine bone marrow in the presence of murine SeroBlock (BUF041A).)
CD169, Monoclonal Antibody (Cat# AAA225720)
Full Name
Rat Anti Mouse CD169: RPE
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Testing Data
(Published customer image: Rat anti Human HLA-DR antibody, clone YE2/36-HLK used for flow cytometryImage caption:BILF1 identified as a lytic gene that downregulates surface MHC class I. 293 (A) or MJS (B) cells were transfected with different EBV genes in the bi-cistronic vector, pCDNA3-IRES-nlsGFP. At 48 hr post-transfection, surface MHC class I was stained with PE-conjugated W6/32 mAb and (in MJS only) MHC class II was stained with PE-conjugated anti-DR mAb, YE2/36-HLK. Two-colour flow cytometry was used to analyse staining in the untransfected GFP- population, shown as the solid line histogram, and in the transfected GFP+ population, shown as the dashed line histogram. The grey histogram denotes background staining obtained with an isotype control PE-conjugated antibody.From: Zuo J, Currin A, Griffin BD, Shannon-Lowe C, Thomas WA, et al. (2009) The Epstein-Barr Virus G-Protein-Coupled Receptor Contributes to Immune Evasion by Targeting MHC Class I Molecules for Degradation. PLoS Pathog 5(1): e1000255.)
HLA DR, Monoclonal Antibody (Cat# AAA213126)
Full Name
RAT ANTI HUMAN HLA DR
Gene Names
HLA-DQB1; IDDM1; CELIAC1; HLA-DQB
Applications
FC/FACS
Pricing
$475/0.25 mg | $1,995/5x0.25 mg
Western Blot (WB)
(Western BlotPositive WB detected in: MCF-7 whole cell lysate, Ntera-2 whole cell lysate, A549 whole cell lysateAll lanes: NANOG antibody at 1:500SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 35, 33 kDaObserved band size: 46, 40 kDa)
NANOG, Monoclonal Antibody (Cat# AAA7112725)
Full Name
NANOG Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, ICC, IF, FC/FACS
Purity
>95%
Protein G Purified
Protein G Purified
Pricing
$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of Perilipin A using anti-Perilipin A antibody (MBS1750855).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: mouse liver tissue lysates,Lane 3: human HepG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Perilipin A antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Perilipin A at approximately 62KD. The expected band size for Perilipin A is at 56KD. )
Perilipin A, Polyclonal Antibody (Cat# AAA1750855)
Full Name
Anti-Perilipin A Picoband Antibody
Gene Names
PLIN1; PERI; PLIN; FPLD4
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)
CD18, Monoclonal Antibody (Cat# AAA213057)
Full Name
RAT ANTI HUMAN CD18
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Reactivity
Dog, Guinea Pig
Applications
FC/FACS, IP
Pricing
$475/0.2 mg | $1,995/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Aquaporin 1 using anti- Aquaporin 1 antibody (MBS175198). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Kidney Tissue Lysate, Lane 2: Rat Lung Tissue Lysate, Lane 3: SMMC Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Aquaporin 1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Aquaporin 1 at approximately 28KD. The expected band size for Aquaporin 1 is at 28KD. )
Aquaporin 1, Polyclonal Antibody (Cat# AAA175198)
Full Name
Anti-Aquaporin 1 antibody
Gene Names
AQP1; CO; CHIP28; AQP-CHIP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
