Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
1205 results for "Rat IgG Isotype Control" - showing 100-150
Western Blot (WB)
(Figure 1. Western blot analysis of POR using anti-POR antibody (MBS178155).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Liver Tissue Lysate,Lane 2: Human Placenta Tissue Lysate,Lane 3: HEPG2 Whole Cell Lysate,Lane 4: HEPA Whole Cell Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POR antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for POR at approximately 85KD. The expected band size for POR is at 77KD. )
POR, Polyclonal Antibody (Cat# AAA178155)
Full Name
Anti-POR Antibody
Gene Names
POR; CPR; CYPOR; P450R
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-GITR antibody YGITR860.103.5 Mouse spleenocyts were stained with an isotype control or the rabbit-chimeric version of YGITR860.103.5 at a concentration of 1 ug/ml for 30 mins at RT without (Blue) and with (Grey) stimulation of the leukocytes with immobilized anti-CD3 and anti-CD28 antibodies. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
GITR, Monoclonal Recombinant Antibody (Cat# AAA488307)
Full Name
Anti-GITR [YGITR 860.103.5]
Gene Names
TNFRSF18; GITR
Reactivity
Mouse
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Figure 1. Flow Cytometry analysis of A431 cells using anti-Cytochrome C antibody (M03529-5).Overlay histogram showing A431 cells stained with M03529-5 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytochrome C Antibody (M03529-5, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight® 488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Cytochrome C, Monoclonal Antibody (Cat# AAA1752282)
Full Name
Anti-Cytochrome C Antibody (monoclonal, 15F10)
Gene Names
CYCS; CYC; HCS; THC4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from A549 cell line and human plasma tissue lysate(from left to right), using CLU Antibody (N-term). MBS9215265 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
CLU, Polyclonal Antibody (Cat# AAA9215265)
Full Name
CLU Antibody (N-term)
Gene Names
CLU; CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2
Reactivity
Human, mouse, rat
Applications
FC, WB, IHC-P, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Anti- CD22 Picoband antibody, MBS177673, Western blottingAll lanes: Anti CD22 (MBS177673) at 0.5ug/mlLane 1: MCF-7 Whole Cell Lysate at 40ugLane 2: 22RV1 Whole Cell Lysate at 40ugPredicted bind size: 95KDObserved bind size: 95KD )
CD22, Polyclonal Antibody (Cat# AAA177673)
Full Name
Anti-CD22 Antibody
Gene Names
CD22; SIGLEC2; SIGLEC-2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(All lanes : Anti-WNT5A Antibody (Center) at 1:2000 dilution Lane 1: human heart lysate Lane 2: PANC-1 whole cell lysate Lane 3: mouse heart lysate Lane 4: rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WNT5A, Polyclonal Antibody (Cat# AAA9210555)
Full Name
WNT5A Antibody (Center)
Gene Names
WNT5A; hWNT5A
Reactivity
Human, Mouse, Rat
Predicted: Rabbit
Predicted: Rabbit
Applications
EIA, IHC-P, IHC-P-Leica, FC/FACS, WB
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-GITR antibody YGITR765 Mouse activated T cells were stained with an isotype control or the rabbit-chimeric version of YGITR765 at a concentration of 1 ug/ml for 30 mins at RT without (Blue) and with (Grey) stimulation of the lymphocytes with immobilized anti-CD3 and anti-CD28 antibodies. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
GITR, Monoclonal Recombinant Antibody (Cat# AAA488333)
Full Name
Anti-GITR [YGITR765]
Gene Names
TNFRSF18; GITR
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of NFAT2 using anti-NFAT2 antibody (MBS1750373).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse thymus tissue lysates,Lane 2: human 22RV1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFAT2 at approximately 101KD. The expected band size for NFAT2 is at 101KD. )
NFAT2, Polyclonal Antibody (Cat# AAA1750373)
Full Name
Anti-NFAT2 Picoband Antibody
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Interleukin 7 receptor antibody YIL7R323 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of YIL7R323 (MBS488434) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488434, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Interleukin 7 Receptor, Monoclonal Recombinant Antibody (Cat# AAA488434)
Full Name
Anti-Interleukin 7 Receptor [YIL7R323]
Gene Names
IL7R; ILRA; CD127; IL7RA; CDW127; IL-7R-alpha
Reactivity
Human
Applications
FC/FACS, EIA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
SDS-Page
(SDS-PAGE Analysis Purified HER-4/ERBB4 Mouse Monoclonal Antibody (HFR-1). Confirmation of Purity and Integrity of Antibody.)
HER-4/ERBB4, Monoclonal Antibody (Cat# AAA4380869)
Full Name
HER-4/ERBB4
Gene Names
ERBB4; HER4; ALS19; p180erbB4
Reactivity
Human, Mouse
Predicted to work with Rat, Chicken.
Predicted to work with Rat, Chicken.
Applications
FC/FACS, IF
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Flow Cytometry (FC/FACS)
(Figure 1. Flow Cytometry analysis of U20S cells using anti-GSTM1 antibody (M00569).Overlay histogram showing U20S cells stained with M00569 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GSTM1 Antibody (M00569, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GSTM1, Monoclonal Antibody (Cat# AAA1751984)
Full Name
Anti-GSTM1 Antibody (monoclonal, 11F2)
Gene Names
GSTM1; MU; H-B; GST1; GTH4; GTM1; MU-1; GSTM1-1; GSTM1a-1a; GSTM1b-1b
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-YARS monoclonal antibody. )
YARS, Monoclonal Antibody (Cat# AAA120005)
Full Name
Mouse monoclonal antibody Anti-Human YARS
Gene Names
YARS; YRS; YTS; TYRRS; CMTDIC
Reactivity
Human
Applications
ICC, IP, WB, FC/FACS
Western Blot (WB)
(Figure 1. Western blot analysis of Factor I using anti- Factor I antibody (MBS178435). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Factor I antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Factor I at approximately 75KD; 45KD. The expected band size for Factor I is at 66KD. )
Factor I, Polyclonal Antibody (Cat# AAA178435)
Full Name
Anti-Factor I Antibody
Gene Names
CFI; FI; IF; KAF; AHUS3; ARMD13; C3BINA; C3b-INA
Reactivity
Human, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (MBS178597).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates,Lane 2: JURKAT whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD147/Emmprin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD147/Emmprin at approximately 55KD. The expected band size for CD147/Emmprin is at 42KD. )
CD147/Emmprin, Polyclonal Antibody (Cat# AAA178597)
Full Name
Anti-CD147/Emmprin Antibody
Gene Names
BSG; OK; 5F7; TCSF; CD147; EMMPRIN
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Flow Cytometry
(Overlay histogram showing HepG2 cells stained with MBS9215444 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9215444, 1:25 dilution) for 60 min at 37 C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(at 1/400 dilution for 40 min at 37 C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
RAB3B, Monoclonal Antibody (Cat# AAA9215444)
Full Name
RAB3B Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, FC, IHC-P
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of AIRE using anti- AIRE antibody (MBS178480). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- AIRE antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AIRE at approximately 58KD. The expected band size for AIRE is at 58KD. )
AIRE, Polyclonal Antibody (Cat# AAA178480)
Full Name
Anti-AIRE Antibody
Gene Names
AIRE; APS1; APSI; PGA1; AIRE1; APECED
Reactivity
Human, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Anti-IL-17 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml.)
IL17A, Monoclonal Antibody (Cat# AAA246549)
Full Name
Mouse Monoclonal [clone 4K5F6] (IgG2b,k) to Human IL17A
Gene Names
IL17A; IL17; CTLA8; IL-17; IL-17A
Reactivity
Human
Applications
IHC - Paraffin, WB, FC/FACS
Purity
Protein G Purified
Pricing
$585/0.05 mg | $2,585/5x0.05 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Cyclin D3 using anti-Cyclin D3 antibody (MBS177055). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human Cyclin D3 protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin D3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cyclin D3 at approximately 25KD. The expected band size for Cyclin D3 is at 25KD. )
Cyclin D3, Polyclonal Antibody (Cat# AAA177055)
Full Name
Anti-Cyclin D3 Antibody
Reactivity
Human, Rat
Applications
WB
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of ATG14L using anti-ATG14L antibody (MBS178045). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: HELA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG14L antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATG14L at approximately 59KD. The expected band size for ATG14L is at 59KD. )
ATG14L, Polyclonal Antibody (Cat# AAA178045)
Full Name
Anti-ATG14L Antibody
Gene Names
ATG14; ATG14L; BARKOR; KIAA0831
Reactivity
Human, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Anti-Integrin Beta 4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)
ITGB4 / Integrin Beta 4, Monoclonal Antibody (Cat# AAA245779)
Full Name
Rat Monoclonal [clone 439-9B] (IgG2b) to Human ITGB4 / Integrin Beta 4
Gene Names
ITGB4; CD104
Reactivity
Human
Applications
IHC - Paraffin; IHC - Frozen, WB, FC/FACS
Purity
Affinity Purified
Pricing
$585/0.1 mg | $2,585/5x0.1 mg
Western Blot (WB)
(Anti-PPP1R12A antibody, MBS175310, Western blottingAll lanes: Anti PPP1R12A (MBS175310) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: 293T Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugPredicted bind size: 115KDObserved bind size: 115KD)
Myosin Phosphatase, Polyclonal Antibody (Cat# AAA175310)
Full Name
Anti-Myosin Phosphatase antibody
Gene Names
PPP1R12A; MBS; M130; MYPT1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of DBI using anti-DBI antibody (MBS178641). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBI antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DBI at approximately 10KD. The expected band size for DBI is at 10KD. )
DBI, Polyclonal Antibody (Cat# AAA178641)
Full Name
Anti-DBI Antibody
Gene Names
DBI; EP; ACBP; ACBD1; CCK-RP
Reactivity
Human
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Figure 1. IHC analysis of CD62P using anti-CD62P antibody (MBS177774).CD62P was detected in paraffin-embedded section of Human Tonsil Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD62P Antibody (MBS177774) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
CD62P, Polyclonal Antibody (Cat# AAA177774)
Full Name
Anti-CD62P Antibody
Gene Names
SELP; CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
Reactivity
Human
Applications
WB, IHC, EIA
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunofluorescence (IF)
( Immunofluorescence staining of fixed mouse splenocytes with anti-GITRL (Tumor necrosis factor ligand superfamily member 18) antibody YGL386 Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of YGL386 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
GITRL, Monoclonal Recombinant Antibody (Cat# AAA488331)
Full Name
Anti-GITRL [YGL386]
Gene Names
TNFSF18; TL6; AITRL; GITRL; TNLG2A; hGITRL
Reactivity
Mouse
Applications
FC/FACS, EIA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-SMAD1 monoclonal antibody. )
SMAD1, Monoclonal Antibody (Cat# AAA120248)
Full Name
Mouse monoclonal antibody Anti-Human SMAD1
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human
Applications
DB, ICC, IP, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Testing Data
(Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)
CD18, Monoclonal Antibody (Cat# AAA219819)
Full Name
RAT ANTI HUMAN CD18:Low Endotoxin
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS, FN, IP
Pricing
$745/0.5 mg | $3,200/5x0.5 mg
Testing Data
(Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD4 and RPE conjugated Rat IgG2b isotype control . Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD4 and RPE conjugated Mouse anti Human CD45 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)
CD45, Monoclonal Antibody (Cat# AAA225669)
Full Name
Rat Anti Human CD45: FITC
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Flow Cytometry (FC/FACS)
(Overlay histogram showing NIH/3T3 cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
beta Tubulin, Polyclonal Antibody (Cat# AAA9232228)
Full Name
beta Tubulin
Gene Names
Tubb5; AA408537; AI596182; M(beta)5; B130022C14Rik
Reactivity
Human, Mouse, Rat
Predicted: C.Elegans, Chicken, Drosophila, Monkey, Bovine, Xenopus, Hamster, Pig
Predicted: C.Elegans, Chicken, Drosophila, Monkey, Bovine, Xenopus, Hamster, Pig
Applications
WB, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215791)
Full Name
RAT ANTI MOUSE DECTIN-1:Biotin
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$210/0.025 mg | $800/5x0.025 mg
Western Blot (WB)
(Western blot analysis of anti-CSF1R Pab in human placenta. CSF1R (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
MCSF Receptor (CSF1R), Polyclonal Antibody (Cat# AAA9211301)
Full Name
MCSF Receptor (CSF1R) Antibody (C-term)
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
WB, EIA, FC, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-complement receptors 1 & 2 antibody 7G6 Mouse lymphocytes were stained with an isotype control or the rabbit-chimeric version of 7G6 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
Complement Receptor 1 & 2, Recombinant Antibody (Cat# AAA488171)
Full Name
Anti-Complement Receptor 1 & 2 [7G6]
Reactivity
Mouse
Applications
FC/FACS, IHC, IP, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Integrin beta-7 antibody FIB27 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB27 (MBS488444) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488444, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Integrin beta-7, Monoclonal Recombinant Antibody (Cat# AAA488444)
Full Name
Anti-Integrin beta-7 [FIB27]
Reactivity
Human, Mouse
Applications
FC/FACS, IP, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of beta Catenin using anti-beta Catenin antibody (M00004-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: human COLO-320 whole cell lysates, Lane 3: human HepG2 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: human SW620 whole cell lysates,Lane 6: human 22RV1 whole cell lysates,Lane 7: human Jurkat whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-beta Catenin antigen affinity purified monoclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.)
beta Catenin, Monoclonal Antibody (Cat# AAA1751889)
Full Name
Anti-beta Catenin Antibody (monoclonal, 1F6)
Gene Names
CTNNB1; EVR7; CTNNB; MRD19; armadillo
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(All lanes : Anti-MCL1 Antibody (BH3 Domain Specific) at 1:2000 dilution Lane 1: Raji whole cell lysate Lane 2: Ramos whole cell lysate Lane 3: A431 whole cell lysate Lane 4: F9 whole cell lysate Lane 5: A20 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
MCL1, Polyclonal Antibody (Cat# AAA9206247)
Full Name
MCL1 Antibody (BH3 Domain Specific)
Gene Names
MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC-P, FC, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Flow Cytometry (FC/FACS)
(Overlay histogram showing NIH/3T3 cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
beta II Tubulin, Polyclonal Antibody (Cat# AAA9232227)
Full Name
beta II Tubulin Antibody
Gene Names
Tubb2a; Tubb2; M(beta)2
Reactivity
Human, Mouse, Rat
Predicted: Chicken, Monkey, Bovine, Xenopus, Pig
Predicted: Chicken, Monkey, Bovine, Xenopus, Pig
Applications
WB, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of ADK using anti- ADK antibody (MBS178787). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- ADK antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADK at approximately 40KD. The expected band size for ADK is at40KD. )
ADK, Polyclonal Antibody (Cat# AAA178787)
Full Name
Anti-ADK Antibody
Gene Names
ADK; AK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
SDS-Page
(SDS-PAGE Analysis Purified c-Myc Rabbit Recombinant Monoclonal Antibody (MYC2895R). Confirmation of Purity and Integrity of Antibody.)
c-Myc Oncoprotein, Antibody (Cat# AAA4381109)
Full Name
c-Myc Oncoprotein
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human
Does not react with Mouse, Rat or Chicken.
Does not react with Mouse, Rat or Chicken.
Applications
EIA, FC/FACS, IF
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(All lanes : Anti-VCP Antibody at1:4000 dilutionLane 1: A549 whole cell lysateLane 2: C6 whole cell lysateLane 3: Hela whole cell lysateLane 4: K562 whole cell lysateLane 5: NIH/3T3 whole cell lysateLane 6: U-87 MG whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 89 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
VCP, Monoclonal Antibody (Cat# AAA9232200)
Full Name
VCP Antibody
Gene Names
VCP; p97; TERA; CDC48
Reactivity
Human, Mouse, Rat
Predicted: Dog
Predicted: Dog
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Testing Data
(Published customer image: Rat anti Human HLA-DR antibody, clone YE2/36-HLK used for flow cytometryImage caption:BILF1 identified as a lytic gene that downregulates surface MHC class I. 293 (A) or MJS (B) cells were transfected with different EBV genes in the bi-cistronic vector, pCDNA3-IRES-nlsGFP. At 48 hr post-transfection, surface MHC class I was stained with PE-conjugated W6/32 mAb and (in MJS only) MHC class II was stained with PE-conjugated anti-DR mAb, YE2/36-HLK. Two-colour flow cytometry was used to analyse staining in the untransfected GFP- population, shown as the solid line histogram, and in the transfected GFP+ population, shown as the dashed line histogram. The grey histogram denotes background staining obtained with an isotype control PE-conjugated antibody.From: Zuo J, Currin A, Griffin BD, Shannon-Lowe C, Thomas WA, et al. (2009) The Epstein-Barr Virus G-Protein-Coupled Receptor Contributes to Immune Evasion by Targeting MHC Class I Molecules for Degradation. PLoS Pathog 5(1): e1000255.)
HLA DR, Monoclonal Antibody (Cat# AAA213868)
Full Name
RAT ANTI HUMAN HLA DR:RPE
Gene Names
HLA-DQB1; IDDM1; CELIAC1; HLA-DQB
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Western Blot (WB)
(Figure 1. Western blot analysis of ABCB11 using anti-ABCB11 antibody (MBS177975).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Liver Tissue LysateLane 2: Mouse Liver Tissue LysateLane 3: SMMC Whole Cell LysateAfter Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB11 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ABCB11 at approximately 146KD. The expected band size for ABCB11 is at 146KD. )
ABCB11, Polyclonal Antibody (Cat# AAA177975)
Full Name
Anti-ABCB11 Antibody
Gene Names
ABCB11; BSEP; PGY4; SPGP; ABC16; BRIC2; PFIC2; PFIC-2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD4 and RPE conjugated Rat IgG2b isotype control . Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD4 and RPE conjugated Mouse anti Human CD45 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)
CD45, Monoclonal Antibody (Cat# AAA225670)
Full Name
Rat Anti Human CD45: RPE
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Pricing
$500/100 Tests | $2,090/5x100 Tests
Western Blot (WB)
(Figure 1. Western blot analysis of IDE using anti-IDE antibody (MBS1750623).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human placenta tissue lysates,Lane 3: human COLO-320 whole cell lysates,Lane 4: human HepG2 whole cell lysates,Lane 5: human SGC-7901 whole cell lysates,Lane 6: human Jurkat whole cell lysates,Lane 7: rat stomach tissue lysates,Lane 8: mouse skeletal muscle tissue lysates,Lane 9: mouse stomach tissue lysates,Lane 10: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDE antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IDE at approximately 118KD. The expected band size for IDE is at 118KD. )
IDE, Polyclonal Antibody (Cat# AAA1750623)
Full Name
Anti-IDE Picoband antibody
Gene Names
IDE; INSULYSIN
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of ATP citrate lyase using anti- ATP citrate lyase antibody (MBS178780). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- ATP citrate lyase antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATP citrate lyase at approximately 127KD. The expected band size for ATP citrate lyase is at 127KD. )
ATP citrate lyase, Polyclonal Antibody (Cat# AAA178780)
Full Name
Anti-ATP citrate lyase Antibody
Gene Names
ACLY; ACL; ATPCL; CLATP
Reactivity
Human, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215792)
Full Name
RAT ANTI MOUSE DECTIN-1:Low Endotoxin
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS, FN, IP
Pricing
$830/0.5 mg | $3,580/5x0.5 mg
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-PAX8 monoclonal antibody. )
PAX8, Monoclonal Antibody (Cat# AAA120074)
Full Name
Mouse monoclonal antibody Anti-Human PAX8
Reactivity
Human
Applications
DB, ICC, IP, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
SDS-Page
(SDS-PAGE Analysis Purified GRP94 Recombinant Rabbit Monoclonal (HSP90B1/3168R). Confirmation of Purity and Integrity of Antibody.)
GRP94/HSP90B1, Antibody (Cat# AAA4381313)
Full Name
GRP94/HSP90B1 (Endoplasmic Reticulum Marker)
Gene Names
HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
Reactivity
Human, Monkey, Horse, Cow, Sheep, Pig, Rabbit, Dog, Rat, Mouse, Hamster, Guinea Pig, Chicken, Xenopus laevis
Applications
FC/FACS, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)
Immunofluorescence (IF)
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)
Ly-6C, Monoclonal Antibody (Cat# AAA225659)
Full Name
Rat Anti Mouse Ly-6C: FITC
Gene Names
Ly6c2; Ly-6C2; Ly-6C.2
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215795)
Full Name
RAT ANTI MOUSE DECTIN-1:FITC
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$695/0.5 mg | $2,975/5x0.5 mg
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-CDC5L monoclonal antibody. )
CDC5L, Monoclonal Antibody (Cat# AAA120141)
Full Name
Mouse monoclonal antibody Anti-Human CDC5L
Gene Names
CDC5L; CDC5; CEF1; PCDC5RP; CDC5-LIKE; dJ319D22.1
Reactivity
Human
Applications
DB, ICC, IP, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of NFAT4 using anti-NFAT4 antibody (MBS1750786).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human 22RV1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT4 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFAT4 at approximately 115KD. The expected band size for NFAT4 is at 115KD. )
NFAT4, Polyclonal Antibody (Cat# AAA1750786)
Full Name
Anti-NFAT4 Picoband Antibody
Gene Names
NFATC3; NFAT4; NFATX
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
