Mouse Vimentin Monoclonal Antibody | anti-VIM antibody

Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody

Cat. Num. AAA438228

• Gene Names: VIM; HEL113; CTRCT30
• Reactivity: Human, Cow, Dog, Cat, Pig, Goat, Chicken.; Does not react with Mouse and Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Synonyms: Vimentin; Monoclonal Antibody; Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody; VIM; anti-VIM antibody

• Host: Mouse
• Reactivity: Human, Cow, Dog, Cat, Pig, Goat, Chicken.; Does not react with Mouse and Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: VM452
• Specificity: This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 466

• Applicable Applications for anti-VIM antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (1-2ug/ml); Western Blot (0.25-0.5ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cytoplasmic
• Immunogen: Recombinant full-length human vimentin protein
• Hu-Chromosome Location: 10p12.33
• Positive Control: Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Western Blot (WB)

(Western Blot Analysis Raji Cell Lysate, Vimentin Mouse Monoclonal Antibody (VM452))

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Mouse Monoclonal Antibody (VM452))

Western Blot (WB)

(Western Blot Analysis of human A375 cell lysate using Vimentin Mouse Monoclonal Antibody (VM452))

SDS-PAGE

(SDS-PAGE Analysis Purified Vimentin Mouse Monoclonal Antibody (VM452). Confirmation of Integrity and Purity of Antibody.)

Flow Cytometry (FC)

(Flow Cytometry of human Vimentin on Jurkat Cells, Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Mouse Monoclonal Antibody (VM452).)

Strength of Signal

(Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin (VIM) Mouse Monoclonal Antibody (VM452)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

References

Osborn M et. al. European Journal of Cell Biology. 1984; 34:137-143

NCBI and Uniprot Product Information

• NCBI GI #: 62414289
• NCBI GeneID: 7431
• NCBI Accession #: NP_003371.2
• NCBI GenBank Nucleotide #: NM_003380.3
• UniProt Accession #: P08670; Q15867; Q15868; Q15869; Q548L2; Q6LER9; Q8N850; Q96ML2; Q9NTM3; B0YJC2; D3DRU4
• Molecular Weight: 57-60kDa
• NCBI Official Full Name: vimentin
• NCBI Official Synonym Full Names: vimentin
• NCBI Official Symbol: VIM
• NCBI Official Synonym Symbols: HEL113; CTRCT30
• NCBI Protein Information: vimentin
• UniProt Protein Name: Vimentin
• Protein Family: Vimentin
• UniProt Gene Name: VIM
• UniProt Entry Name: VIME_HUMAN

NCBI Description

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Uniprot Description

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.