Testing Data
(Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)
Rat anti-Mouse CD8 ALPHA Monoclonal Antibody | anti-CD8 antibody
RAT ANTI MOUSE CD8
Suggested Dilution1/50-1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Shelf Life: 12 months from date of despatch.
Testing Data
(Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)
Testing Data
(Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)
Testing Data
(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti mouse CD19, clone 6D5 (MBS212149), green in A and Rat anti Mouse CD8 , red in B. Merged image in C wih nuclei counterstained blue using DAPI. Low power)
Testing Data
(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti mouse CD19, clone 6D5 (MBS212149), green in A and Rat anti Mouse CD8 , red in B. Merged image in C wih nuclei counterstained blue using DAPI. Low power)
Testing Data
(Staining of mouse spleen cells with Rat anti Mouse CD8 (MBS212026))
Testing Data
(Staining of mouse spleen cells with Rat anti Mouse CD8 (MBS212026))
Testing Data
(Immunoperoxidase staining of Mouse lymph node cryosection using Rat anti Mouse CD8alpha antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195) for detection. Low power)
Testing Data
(Immunoperoxidase staining of Mouse lymph node cryosection using Rat anti Mouse CD8alpha antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195) for detection. Low power)
Testing Data
(Immunofluorescence staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody , green in A and Rat anti Mouse CD8 antibody , red in B, Merged image in C)
Testing Data
(Immunofluorescence staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody , green in A and Rat anti Mouse CD8 antibody , red in B, Merged image in C)
Testing Data
(Published customer image: Recruitment of inflammatory cells in the intestine of neonates after CpG-ODN treatment. Eight-day-old neonatal mice received 20 ug/g of CpG-ODN orally. Twenty-four hours after treatment, ilea were removed to analyze the recruitment of inflammatory cells by immunohistology. Six to ten neonates from different litters (5 sections for each animal) per group were analyzed. Data were analyzed by non-parametric Mann-Whitney test.From: Lacroix-Lamand© S, Rochereau N, Mancassola R, Barrier M, Clauzon A, et al. (2009) Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation. PLoS ONE 4(12): e8291.)
Testing Data
(Published customer image: Recruitment of inflammatory cells in the intestine of neonates after CpG-ODN treatment. Eight-day-old neonatal mice received 20 ug/g of CpG-ODN orally. Twenty-four hours after treatment, ilea were removed to analyze the recruitment of inflammatory cells by immunohistology. Six to ten neonates from different litters (5 sections for each animal) per group were analyzed. Data were analyzed by non-parametric Mann-Whitney test.From: Lacroix-Lamand© S, Rochereau N, Mancassola R, Barrier M, Clauzon A, et al. (2009) Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation. PLoS ONE 4(12): e8291.)
Testing Data
(immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse Ly-6B.2 antibody, clone 7/4 (MBS216530), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
Testing Data
(immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse Ly-6B.2 antibody, clone 7/4 (MBS216530), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
Testing Data
(Published customer image: The effect of NKG2D-blocking antibody on NKG2D-positive CD8+T cells and CD8+T cell accumulation in tumors. 4T-1 tumor -bearing mice were subjected to one of two treatments: Dox plus IL-12 plus control IgG or Dox plus IL-12 plus NKG2D-blocking antibody (n = 3 per treatment). (A) NKG2D expression in CD8+T cells was determined as described for Figure 1 (n.s., not significant). (B) The presence of NKG2D/CD8 -positive cells in tumor sections after the indicated treatments were determined as described for Figure 3.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)
Testing Data
(Published customer image: The effect of NKG2D-blocking antibody on NKG2D-positive CD8+T cells and CD8+T cell accumulation in tumors. 4T-1 tumor -bearing mice were subjected to one of two treatments: Dox plus IL-12 plus control IgG or Dox plus IL-12 plus NKG2D-blocking antibody (n = 3 per treatment). (A) NKG2D expression in CD8+T cells was determined as described for Figure 1 (n.s., not significant). (B) The presence of NKG2D/CD8 -positive cells in tumor sections after the indicated treatments were determined as described for Figure 3.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)
Testing Data
(Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 (MBS216530), green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)
Testing Data
(Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 (MBS216530), green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)
Testing Data
(Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 (MBS216530), green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
Testing Data
(Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 (MBS216530), green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
NCBI and Uniprot Product Information
Uniprot Description
CD8A: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane
Molecular Function: protein binding; protein homodimerization activity; protein kinase binding
Biological Process: cell surface receptor linked signal transduction; T cell activation; immune system process; T cell mediated immunity; positive regulation of calcium-mediated signaling; cytotoxic T cell differentiation; defense response to virus
Research Articles on CD8
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Product Notes
The CD8 cd8a (Catalog #AAA214794) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The RAT ANTI MOUSE CD8 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD8 ALPHA can be used in a range of immunoassay formats including, but not limited to, Immunohistology-Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested Dilution1/50-1/100 Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls. Researchers should empirically determine the suitability of the CD8 cd8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8 ALPHA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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