Mouse Human Nuclear Antigen Monoclonal Antibody

Human Nuclear Antigen

Cat. Num. AAA438074

• Reactivity: Human and Non-human primates. Does not react with mouse, rat, and chicken. Others not known.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry
• Synonyms: Human Nuclear Antigen; Monoclonal Antibody; HNA; HuNu; anti-Human Nuclear Antigen antibody

• Host: Mouse
• Reactivity: Human and Non-human primates. Does not react with mouse, rat, and chicken. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 235-1
• Specificity: This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. ; Also available WITHOUT BSA & azide at 1.0mg/ml.

• Applicable Applications for anti-Human Nuclear Antigen antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
• Application Notes: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunoprecipitation (1-2ug/500ug protein lysate); Immunocytochemistry (Acetone-fixed cells) (1-2ug/ml for 30 minutes at RT); Immunohistochemistry (Frozen) (1-2ug/ml for 30 minutes at RT); ; Optimal dilution for a specific application should be determined.
• Immunogen: Nuclei of human myeloid leukemia biopsy cells
• Cellular Localization: Nuclei
• Positive Control: All human cells. Tonsil.
• Preparation and Storage: Antibody with azide store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunofluorescence (IF)

(Immunofluorescent staining of paraformaldehyde-fixed HeLa cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-Mouse IgG-CF488 (Green). Counter stained with Phalloidin (red).)

Flow Cytometry (FC/FACS)

(Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

Immunohistochemistry (IHC)

(Acetone-fixed frozen human Tonsil stained with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1).)

SDS-PAGE

(SDS-PAGE Analysis Purified Human Nuclear Antigen Mouse Monoclonal Antibody (235-1). Confirmation of Purity and Integrity of Antibody.)

Flow Cytometry (FC)

(Flow Cytometric Analysis of PFA-fixed Jurkat cells with Human Nuclear Antigen Mouse Monoclonal Antibody; followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).)

Flow Cytometry (FC)

(Flow Cytometric Analysis of PFA-fixed K562 cells with Human Nuclear Antigen Mouse Monoclonal Antibody; followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).)

Flow Cytometry (FC)

(Flow Cytometric Analysis of PFA-fixed MCF-7 cells with Human Nuclear Antigen Mouse Monoclonal Antibody; followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).)

Immunofluorescence (IF)

(Immunofluorescent staining of PFA-fixed K562 cells with Human Nuclear Antigen Mouse Monoclonal Antibody; followed by goat anti-mouse IgG-CF488 (Green). Counterstained with Phalloidin (red).)

Immunofluorescence (IF)

(Immunofluorescent staining of PFA-fixed MCF-7 cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1); followed by goat anti-mouse IgG-CF488 (Green). Counterstained with Phalloidin (red).)

Flow Cytometry (FC)

(Flow Cytometric Analysis of PFA-fixed Raji cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).)

References

1. Rosario Sanchez-Pernaute et. al. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease. Brain. 2008; 131(8): 2127-2139.