Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Monoclonal Antibody (9G10.F8.2).)
Rat GRP94 / HSP90B1 Monoclonal Antibody | anti-HSP90B1 antibody
GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Rat Monoclonal Antibody
Immunofluorescence (1-2ug/ml)
Western Blot (0.5-1.0ug/ml)
Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Monoclonal Antibody (9G10.F8.2).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Monoclonal Antibody (9G10.F8.2).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Rat Monoclonal Antibody (9G10.F8.2).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Rat Monoclonal Antibody (9G10.F8.2).)
Immunofluorescence (IF)
(Immunofluorescence Analysis of HePG2 cells labeling GRP94 with GRP94 Rat Monoclonal Antibody (9G10.F8.2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot.(Red).)
Immunofluorescence (IF)
(Immunofluorescence Analysis of HePG2 cells labeling GRP94 with GRP94 Rat Monoclonal Antibody (9G10.F8.2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot.(Red).)
Flow Cytometry (FC)
(Flow Cytometric Analysis of PFA-fixed HePG2 cells using GRP94 Rat Monoclonal Antibody (9G10.F8.2) followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).)
Flow Cytometry (FC)
(Flow Cytometric Analysis of PFA-fixed HePG2 cells using GRP94 Rat Monoclonal Antibody (9G10.F8.2) followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]
Uniprot Description
GRP94: Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP. Interacts with TLR4 and TLR9, but not with TLR3. Belongs to the heat shock protein 90 family.
Protein type: Secreted; Apoptosis; Secreted, signal peptide; Heat shock protein
Chromosomal Location of Human Ortholog: 12q24.2-q24.3
Cellular Component: cytosol; endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum membrane; extracellular matrix; extracellular region; focal adhesion; membrane; midbody; nucleus; perinuclear region of cytoplasm
Molecular Function: calcium ion binding; low-density lipoprotein receptor binding; protein binding; protein phosphatase binding; RNA binding; virion binding
Biological Process: actin rod formation; ER-associated protein catabolic process; negative regulation of apoptosis; receptor-mediated endocytosis; regulation of phosphoprotein phosphatase activity; response to hypoxia; retrograde protein transport, ER to cytosol; toll-like receptor signaling pathway
Research Articles on HSP90B1
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Product Notes
The HSP90B1 hsp90b1 (Catalog #AAA439463) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Rat Monoclonal Antibody reacts with Human, Monkey, Horse, Cow, Sheep, Pig, Rabbit, Dog, Rat, Mouse, Hamster, Guinea Pig, Chicken, Xenopus laevis and may cross-react with other species as described in the data sheet. AAA Biotech's GRP94 / HSP90B1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin. Flow Cytometry (0.5-1ug/million cells in 0.1ml) Immunofluorescence (1-2ug/ml) Western Blot (0.5-1.0ug/ml) Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the HSP90B1 hsp90b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRP94 / HSP90B1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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