Flow Cytometry (FC/FACS)
(Blocking of Fc-receptors by anti-CD16/32 antibody. Ex vivo murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate, left and middle panel) as well as bone marrow-derived macrophages (BMDMs, right panel) were stained with a murine anti-human antibody (fluorescently labelled (AF647) human-specific anti-CD16) with or without pre-incubation with anti-mouse CD16/CD32 (Fc-receptors) antibody (clone 2.4G2). Pre-incubation of cells with anti-CD16/CD32 reduced non-specific binding of the fluorescently labelled antibody to all cell types analysed and shows that this anti-CD16/CD32 antibody acts as an Fc-blocking reagent.)
CD16/CD32 recombinant antibody
Anti-CD16/CD32 [2.4G2]
Flow Cytometry (FC/FACS)
(Blocking of Fc-receptors by anti-CD16/32 antibody. Ex vivo murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate, left and middle panel) as well as bone marrow-derived macrophages (BMDMs, right panel) were stained with a murine anti-human antibody (fluorescently labelled (AF647) human-specific anti-CD16) with or without pre-incubation with anti-mouse CD16/CD32 (Fc-receptors) antibody (clone 2.4G2). Pre-incubation of cells with anti-CD16/CD32 reduced non-specific binding of the fluorescently labelled antibody to all cell types analysed and shows that this anti-CD16/CD32 antibody acts as an Fc-blocking reagent.)
Flow Cytometry (FC/FACS)
(Blocking of Fc-receptors by anti-CD16/32 antibody. Ex vivo murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate, left and middle panel) as well as bone marrow-derived macrophages (BMDMs, right panel) were stained with a murine anti-human antibody (fluorescently labelled (AF647) human-specific anti-CD16) with or without pre-incubation with anti-mouse CD16/CD32 (Fc-receptors) antibody (clone 2.4G2). Pre-incubation of cells with anti-CD16/CD32 reduced non-specific binding of the fluorescently labelled antibody to all cell types analysed and shows that this anti-CD16/CD32 antibody acts as an Fc-blocking reagent.)
Flow Cytometry (FC/FACS)
( Anti-CD16/32 staining of murine macrophages Murine bone marrow-derived macrophages (BMDMs) were stained with anti-CD16/32 antibody (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor 647 commercially available from a competitor. Compared to the isotype control and secondary-only control, a CD16/32-positive population is apparent.)
Flow Cytometry (FC/FACS)
( Anti-CD16/32 staining of murine macrophages Murine bone marrow-derived macrophages (BMDMs) were stained with anti-CD16/32 antibody (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor 647 commercially available from a competitor. Compared to the isotype control and secondary-only control, a CD16/32-positive population is apparent.)
NCBI and Uniprot Product Information
Uniprot Description
FCGR2A: low affinity receptor for the Fc region of IgGs. Binding to IgG initiates cellular responses against pathogens and soluble antigens. A type I membrane protein found on monocytes, neutrophils and platelets. SHP-1 associates with this protein to modulate signaling events in myeloid cells.
Protein type: Cell surface; Membrane protein, integral; Receptor, misc.
Cellular Component: external side of plasma membrane
Molecular Function: IgG binding; IgG receptor activity
Biological Process: antibody-dependent cellular cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I; cell surface receptor linked signal transduction; mast cell activation; neutrophil chemotaxis; phagocytosis, engulfment; phagocytosis, recognition; positive regulation of phagocytosis; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of type I hypersensitivity; positive regulation of type IIa hypersensitivity; positive regulation of type III hypersensitivity; regulation of immune response; serotonin secretion
Research Articles on CD16/CD32
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Product Notes
The CD16/CD32 fcgr3 (Catalog #AAA488078) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD16/CD32 [2.4G2] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD16/CD32 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Block. This antibody binds murine Fc Receptors CD16/CD32 and inhibits binding of antibodies to these. It can therefore be used as an Fc-blocking reagent, to reduce non-specific staining of cells by antibodies binding via their Fc-region. A similar effect can be achieved using the Fc Silent range of antibodies that prevent these interactions at the level of the Fc receptor binding site, which has been mutated to lack affinity for the Fc receptors. Researchers should empirically determine the suitability of the CD16/CD32 fcgr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD16/CD32, Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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