Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
1205 results for "Rat IgG Isotype Control" - showing 150-200
Western Blot (WB)
(Figure 1. Western blot analysis of CD2AP using anti-CD2AP antibody (MBS1750710).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,Lane 2: human 293T whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human COLO-320 whole cell lysates,Lane 5: human 22RV1 whole cell lysates,Lane 6: human SK-OV-3 whole cell lysates,Lane 7: rat stomach tissue lysates,Lane 8: rat liver tissue lysates,Lane 9: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD2AP antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD2AP at approximately 85KD. The expected band size for CD2AP is at 71KD. )
CD2AP, Polyclonal Antibody (Cat# AAA1750710)
Full Name
Anti-CD2AP Picoband antibody
Gene Names
CD2AP; CMS
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
ELISA (EIA)
(ELISA of anti-CD27 antibody on CD27-Fc fusion protein, with a CD70 (CD27-L) competition assay. Binding curves of the rabbit IgG chimeric version of the anti-CD27 (Interleukin-2 receptor subunit alpha) antibody RM27-3E5 and isotype control (anti-Fluorescein antibody and then at 3-fold dilutions down to 0.17ng/mL (red line). A negative control assay using 4-1BB Fc-fusion protein was also performed (yellow line). Inhibition of the binding to Pr00160-1.9 (CD27) by (CD70) may be observed between 10,000 ng/mL and 123 ng/mL, whereas does not inhibit a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.)
CD27, Monoclonal Recombinant Antibody (Cat# AAA488375)
Full Name
Anti-CD27 [RM27-3E5]
Gene Names
Cd27; S152; Tp55; Tnfrsf7
Reactivity
Mouse
Applications
FC/FACS, IP
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-TCERG1 monoclonal antibody. )
TCERG1, Monoclonal Antibody (Cat# AAA120042)
Full Name
Mouse monoclonal antibody Anti-Human TCERG1
Gene Names
TCERG1; Urn1; CA150; TAF2S
Reactivity
Human
Applications
EIA, ICC, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Recombinant Rabbit Monoclonal Antibody (CLIP/3127R).)
CD74, Antibody (Cat# AAA4381446)
Full Name
CD74 (B-Cell Marker)
Gene Names
CD74; II; p33; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human, Baboon, Mouse
Does not react with Rat.
Does not react with Rat.
Applications
FC/FACS, WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot analysis of APOA1 expression in human placenta extract (lane 1). APOA1 at 26KD was detected using rabbit anti- APOA1 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Apolipoprotein A I, Polyclonal Antibody (Cat# AAA178416)
Full Name
Anti-Apolipoprotein A I Antibody
Gene Names
APOA1; apo(a)
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB, IHC, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody , red in A and Mouse anti Rat CD4 , green in B. C is the Merged image with nuclei counter-stained blue using DAPI. High power)
CD172a, Monoclonal Antibody (Cat# AAA210478)
Full Name
MOUSE ANTI RAT CD172a:Biotin
Gene Names
Sirpa; Bit; Ptpns1; SHPS-1
Applications
FC/FACS
Pricing
$425/0.1 mg | $1,755/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PRX using anti-PRX antibody (MBS1750695).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,Lane 2: mouse kidney tissue lysates,Lane 3: human 293T whole cell lysates,Lane 4: human HK-2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRX antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRX at approximately 155KD. The expected band size for PRX is at 155KD. )
PRX, Polyclonal Antibody (Cat# AAA1750695)
Full Name
Anti-PRX Picoband antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Calpastatin using anti-Calpastatin antibody (MBS178604). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: COLO320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calpastatin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Calpastatin at approximately 100KD. The expected band size for Calpastatin is at 76KD. )
Calpastatin, Polyclonal Antibody (Cat# AAA178604)
Full Name
Anti-Calpastatin Antibody
Gene Names
CAST; BS-17; PLACK
Reactivity
Human
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215800)
Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS, IP
Pricing
$210/0.025 mg | $800/5x0.025 mg
Testing Data
(Figure A. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat IgG2a isotype control . Figure B. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat anti Canine CD5 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
IgG2a, Negative Control (Cat# AAA225675)
Full Name
Rat IgG2a Negative Control
Reactivity
Rat
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$215/0.1 mg | $810/5x0.1 mg
Testing Data
(Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody , red in A and Mouse anti Rat CD4 , green in B. C is the Merged image with nuclei counter-stained blue using DAPI. High power)
CD172a, Monoclonal Antibody (Cat# AAA212964)
Full Name
MOUSE ANTI RAT CD172a
Gene Names
Sirpa; Bit; Ptpns1; SHPS-1
Applications
FC/FACS, WB
Pricing
$490/0.25 mg | $2,050/5x0.25 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded M. liver section using Mouse Csnk1g3 Antibody (C-term). MBS9214074 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Csnk1g3, Polyclonal Antibody (Cat# AAA9214074)
Full Name
Mouse Csnk1g3 Antibody (C-term)
Gene Names
Csnk1g3; 3300002K07Rik; C330049O21Rik
Reactivity
Mouse (Predicted Reactivity: Rat)
Applications
EIA, IHC-p, FC/FACS, WB
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of MCF-7 cells using ADRA1D Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9205971 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
ADRA1D, Polyclonal Antibody (Cat# AAA9205971)
Full Name
ADRA1D Antibody (N-term)
Gene Names
ADRA1D; DAR; ADRA1; ADRA1A; ADRA1R; ALPHA1; dJ779E11.2
Reactivity
Human, rat (Predicted Reactivity: Mouse, Bovine, Dog, Rabbit, Sheep)
Applications
FC/FACS, EIA, IHC, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Quality Control
(Left: Western blot analysis of immunized recombinant protein, using anti-DHX38 monoclonal antibody.)
DHX38, Monoclonal Antibody (Cat# AAA120170)
Full Name
Mouse monoclonal antibody Anti-Human DHX38
Gene Names
DHX38; DDX38; PRP16; PRPF16
Reactivity
Human
Applications
DB, ICC, IP, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse anti Human CD29 . All experiments performed on red cell lysed human blood gated on live platelets, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
CD29, Monoclonal Antibody (Cat# AAA225641)
Full Name
Mouse Anti Human CD29: FITC
Gene Names
ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
Reactivity
Human, Mink
Does not react with: Rat, Mouse
Does not react with: Rat, Mouse
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Testing Data
(Published customer image:IL-4 DC and IL-10 DC exhibit no obvious differences in their phenotype. IL-4 DC and IL-10 DC and mature splenic DC (sDC) coexpressed Ox62 and CD68, whereas macrophages generated in the presence of M-CSF (5 ug/ml) were only positive for CD68. Broken lines indicate background staining obtained using an irrelevant isotype control. The first number represents the percentage of cells staining positive for the indicated marker and the second number represents the mean fluorescence intensity. The results shown are representative for 4 independent flow cytometric analyses.From:BMC Res Notes. 2009 Jan 23;2:12.)
OX-62, Monoclonal Antibody (Cat# AAA212003)
Full Name
MOUSE ANTI RAT OX-62
Applications
FC/FACS, IP
Pricing
$345/0.1 mg | $1,405/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from MCF-7,Jurkat,PC-12 cell line (from left to right), using TOP1 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
TOP1, Monoclonal Antibody (Cat# AAA9231643)
Full Name
TOP1 Antibody (N-term)
Gene Names
TOP1; TOPI
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
WB, FC/FACS, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-DDX39 monoclonal antibody. )
DDX39, Monoclonal Antibody (Cat# AAA120168)
Full Name
Mouse monoclonal antibody Anti-Human DDX39
Gene Names
DDX39A; BAT1; DDXL; BAT1L; DDX39; URH49
Reactivity
Human
Applications
WB
Testing Data
(Figure A. RPE conjugated Rat anti Mouse CD3 and FITC conjugated Rat IgG2b isotype control . Figure B. RPE conjugated Rat anti Mouse CD3 and FITC conjugated Rat anti Mouse CD8 . All experiments performed on red cell lysed mouse blood gated on lymphocytes in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.)
CD8, Monoclonal Antibody (Cat# AAA225633)
Full Name
Rat Anti Mouse CD8: FITC
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215789)
Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS, IP
Pricing
$525/0.25 mg | $2,210/5x0.25 mg
Western Blot (WB)
(Figure 1. Western blot analysis of TLR1 using anti-TLR1 antibody (MBS1750402).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates,Lane 2: mouse small intestine tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TLR1 at approximately 90KD. The expected band size for TLR1 is at 90KD. )
TLR1, Polyclonal Antibody (Cat# AAA1750402)
Full Name
Anti-TLR1 Picoband antibody
Gene Names
TLR1; TIL; CD281; rsc786; TIL. LPRS5
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from M.brain tissue,rat PC-12,K562,Hela cell line (from left to right), using PIN1 Antibody. It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
PIN1, Monoclonal Antibody (Cat# AAA9231759)
Full Name
PIN1 Antibody
Gene Names
PIN1; DOD; UBL5
Reactivity
Human, Mouse
Predicted: Rat
Predicted: Rat
Applications
WB, IHC, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Immunohistochemistry (IHC)-Paraffin
(Staining SNCA in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
SNCA, Polyclonal Antibody (Cat# AAA9231880)
Full Name
SNCA Antibody (C-term)
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human, Rat
Predicted: Mouse
Predicted: Mouse
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Flow Cytometry (FC/FACS)
(Overlay histogram showing HL-60 cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was mouse IgG3 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
ALDOC, Monoclonal Antibody (Cat# AAA9232178)
Full Name
ALDOC Antibody (C-term)
Gene Names
ALDOC; ALDC
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Flow Cytometry (FC/FACS)
(Overlay histogram showing Jurkat cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was mouse IgG2a (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Erk1/2, Monoclonal Antibody (Cat# AAA9232168)
Full Name
Erk1/2 Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human
Predicted: Bovine, Mouse, Rat
Predicted: Bovine, Mouse, Rat
Applications
WB, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells using RPS6 Antibody (N-term)(green) compared to an isotype control of mouse IgG1(blue). MBS9200615 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
RPS6, Monoclonal Antibody (Cat# AAA9200615)
Full Name
RPS6 Antibody (N-term)
Gene Names
RPS6; S6
Reactivity
Human, rat (Predicted Reactivity: Monkey, Mouse)
Applications
EIA, IHC, WB, FC/FACS, IF
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-desmosome/hemidesmosome antibody F12 A431 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of F12 (MBS488463, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Desmosome/Hemidesmosome, Monoclonal Recombinant Antibody (Cat# AAA488463)
Full Name
Anti-Desmosome/Hemidesmosome [F12]
Reactivity
Human
Applications
IF, IEM
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Bcl-X using anti- Bcl-X antibody (MBS178417). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Bcl-X antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Bcl-X at approximately 29 KD, 60KD. The expected band size for Bcl-X is at 26KD. )
Bcl-X, Polyclonal Antibody (Cat# AAA178417)
Full Name
Anti-Bcl-X Antibody
Gene Names
BCL2L1; BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Bik using anti-Bik antibody (MBS178226). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Spleen Tissue Lysate, Lane 2: Rat Gaster Tissue Lysate, Lane 3: Rat Intestine Tissue Lysate, Lane 4: MCF-7 Whole Cell Lysate, Lane 5: A549 Whole Cell Lysate, Lane 6: SKOV Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bik antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Bik at approximately 22KD. The expected band size for Bik is at 22KD. )
Bik, Polyclonal Antibody (Cat# AAA178226)
Full Name
Anti-Bik Antibody
Gene Names
BIK; BP4; NBK; BIP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Dynamin 1 using anti-Dynamin 1 antibody (MBS1750776).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates,Lane 3: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dynamin 1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Dynamin 1 at approximately 97KD. The expected band size for Dynamin 1 is at 97KD. )
Dynamin 1, Polyclonal Antibody (Cat# AAA1750776)
Full Name
Anti-Dynamin 1 Picoband antibody
Gene Names
DNM1; DNM; EIEE31
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Figure 1. IHC analysis of TAPA1 using anti-TAPA1 antibody (MBS1750607).TAPA1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TAPA1 Antibody (MBS1750607) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
TAPA1, Polyclonal Antibody (Cat# AAA1750607)
Full Name
Anti-TAPA1 Picoband antibody
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of NFIA using anti- NFIA antibody (MBS178836).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: JURKAT whole cell lysates,Lane 2: COLO320 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- NFIA antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFIA at approximately 69KD. The expected band size for NFIA is at 56KD. )
NFIA, Polyclonal Antibody (Cat# AAA178836)
Full Name
Anti-NFIA Antibody
Gene Names
NFIA; CTF; NF1-A; NFI-A; NFI-L; NF-I/A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from A431,Hela cell line (from left to right), using DFFB Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
DFFB, Polyclonal Antibody (Cat# AAA9231709)
Full Name
DFFB Antibody (Center)
Gene Names
DFFB; CAD; CPAN; DFF2; DFF40; DFF-40
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Flow Cytometry (FC/FACS)
(Overlay histogram showing Hela cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
RPS7, Polyclonal Antibody (Cat# AAA9232223)
Full Name
RPS7 Antibody (N-Term)
Gene Names
RPS7; S7; eS7; DBA8
Reactivity
Human, Mouse
Predicted: Bovine, Zebrafish, Rat, Xenopus
Predicted: Bovine, Zebrafish, Rat, Xenopus
Applications
WB, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Anti- BMP-5 Picoband antibody, MBS178341, Western blottingAll lanes: Anti BMP-5 (MBS178341) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Mouse Liver Tissue Lysate at 50ugLane 3: A549 Whole Cell Lysate at 40ugPredicted bind size: 51KDObserved bind size: 51KD)
BMP5, Polyclonal Antibody (Cat# AAA178341)
Full Name
Anti-BMP5 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from Hela,A431,MCF-7,mouse C2C12,rat C6 cell line (from left to right), using ACTB Antibody. It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Beta-Actin, Monoclonal Antibody (Cat# AAA9231852)
Full Name
Beta-Actin Antibody
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Rat
Predicted: Xenopus, Bovine, Chicken, Horse, Monkey, Mouse, Pig, Sheep
Predicted: Xenopus, Bovine, Chicken, Horse, Monkey, Mouse, Pig, Sheep
Applications
WB, IHC, FC/FACS, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Testing Data
(Figure A. RPE conjugated Mouse anti Human CD19 and FITC conjugated Rat IgG2b isotype control . Figure B. RPE conjugated Mouse anti Human CD19 and FITC conjugated Rat anti Human CD18 . All experiments performed on human peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).)
IgG2b, Negative Control (Cat# AAA225676)
Full Name
Rat IgG2b Negative Control: Pacific Blue
Reactivity
Rat
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$300/100 Tests | $1,200/5x100 Tests
Testing Data
(1:1000 dilution of this antibody detected SMC1A on 40ug of cell lysates)
SMC1A, Monoclonal Antibody (Cat# AAA9400360)
Full Name
SMC1A(N-term) Monoclonal Antibody
Gene Names
SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha
Reactivity
Human, Porcine, Mouse, Rat
Applications
WB, IHC, FC/FACS
Purity
Purified by antigen-affinity chromatography.
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from mouse kidney and liver tissue lysate (from left to right), using Mouse Csnk1g3 Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.)
Csnk1g3, Polyclonal Antibody (Cat# AAA9231587)
Full Name
Mouse Csnk1g3 Antibody (C-term)
Gene Names
Csnk1g3; 3300002K07Rik; C330049O21Rik
Reactivity
Mouse
Predicted: Rat
Predicted: Rat
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of NFATC4 using anti-NFATC4 antibody (MBS1750778).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat testis tissue lysates,Lane 2: mouse testis tissue lysates,Lane 3: human Hela whole cell lysates,Lane 4: human placenta tissue lysates,Lane 5: human A549 whole cell lysates,Lane 6: human SK-OV-3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFATC4 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFATC4 at approximately 95120KD. The expected band size for NFATC4 is at 95KD. )
NFATC4, Polyclonal Antibody (Cat# AAA1750778)
Full Name
Anti-NFATC4 Picoband Antibody
Gene Names
NFATC4; NFAT3; NF-AT3; NF-ATC4
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of ATF2 using anti-ATF2 antibody (MBS177474).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: Recombinant Human ATF2 Protein 0.5ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATF2 at approximately 49KD. The expected band size for ATF2 is at 49KD. )
Cyclic AMP-dependent transcription factor ATF-2, Polyclonal Antibody (Cat# AAA177474)
Full Name
Anti-ATF2 Antibody
Gene Names
ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Applications
WB, IHC-P, IHC-F
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from A431,mouse NIH/3T3,L929,rat C6,Hela cell line (from left to right), using HIST1H4A Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
HIST1H4A, Polyclonal Antibody (Cat# AAA9231704)
Full Name
HIST1H4A Antibody (C-term)
Reactivity
Human, Mouse, Rat
Predicted: Bovine, C.Elegans, Chicken, D, Monkey, Pig, Xenopus, Yeast
Predicted: Bovine, C.Elegans, Chicken, D, Monkey, Pig, Xenopus, Yeast
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of Stefin B using anti-Stefin B antibody (M02794). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Stefin B antigen affinity purified monoclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.)
Stefin B, Monoclonal Antibody (Cat# AAA1752235)
Full Name
Anti-Stefin B Antibody (monoclonal, 2B6)
Gene Names
CSTB; PME; ULD; CST6; EPM1; STFB; CPI-B; EPM1A
Reactivity
Human
Applications
WB, IHC, ICC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CD147 using anti-CD147 antibody (MBS177907).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: Recombinant Human CD147 Protein 0.5ngAfter Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD147 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD147 at approximately39KD. The expected band size for CD147 is at 39KD. )
CD147, Polyclonal Antibody (Cat# AAA177907)
Full Name
Anti-CD147 Antibody
Gene Names
BSG; OK; 5F7; TCSF; CD147; EMMPRIN
Reactivity
Human
Applications
WB
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215798)
Full Name
RAT ANTI MOUSE DECTIN-1:RPE
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$550/100 Tests | $2,320/5x100 Tests
Flow Cytometry (FC/FACS)
(Overlay histogram showing U-87 MG cells stained at 37 degree C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
PLOD1, Polyclonal Antibody (Cat# AAA9231969)
Full Name
PLOD1 Antibody (N-term)
Gene Names
PLOD1; LH; LH1; LLH; EDS6; PLOD; EDSKCL1
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
WB, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Testing Data
(Anti-CD169 staining of murine macrophages Murine bone marrow-derived macrophages (BMDMs) were stained with anti-CD169 antibody (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a CD169-positive population is apparent.)
CD169, Monoclonal Recombinant Antibody (Cat# AAA488383)
Full Name
Anti-CD169 [3D6.112 (Recombinant Version)]
Gene Names
Siglec1; Sn; Cd169; Siglec-1
Reactivity
Mouse
Applications
WB, IHC
Purity
Protein A Affinity Purified
Pricing
$635/0.1 mg | $2,660/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PPT1 using anti- PPT1 antibody (MBS178109).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates,Lane 3: rat liver tissue lysates,Lane 4: mouse liver tissue lysates,Lane 5: HEPG2 whole Cell lysates,Lane 6: 293T whole cell lysates,Lane 7: MCF-7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- PPT1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PPT1. )
PPT1, Polyclonal Antibody (Cat# AAA178109)
Full Name
Anti-PPT1 Antibody
Gene Names
PPT1; PPT; CLN1; INCL
Reactivity
Human, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of DC-SIGN using anti-DC-SIGN antibody (MBS1750548).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human MCF-7 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: rat spleen tissue lysates,Lane 6: mouse thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DC-SIGN antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DC-SIGN at approximately 46KD. The expected band size for DC-SIGN is at 46KD. )
DC-SIGN, Polyclonal Antibody (Cat# AAA1750548)
Full Name
Anti-DC-SIGN Picoband antibody
Gene Names
CD209; CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-ASH2L monoclonal antibody. )
ASH2L, Monoclonal Antibody (Cat# AAA120142)
Full Name
Mouse monoclonal antibody Anti-Human ASH2L
Gene Names
ASH2L; ASH2; Bre2; ASH2L1; ASH2L2
Reactivity
Human
Applications
DB, ICC, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
