Immunohistochemistry
(MBS9217260 staining beta Tubulin in mouse thymus tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Rabbit beta Tubulin Polyclonal Antibody | anti-Tubb5 antibody
beta Tubulin
FC~~1:25
WB~~1:2000
Immunohistochemistry
(MBS9217260 staining beta Tubulin in mouse thymus tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry
(MBS9217260 staining beta Tubulin in mouse thymus tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Flow Cytometry
(Overlay histogram showing C2C12 cells stained with MBS9217260(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specificprotein-protein interactions followed by the antibody (MBS9217260, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Flow Cytometry
(Overlay histogram showing C2C12 cells stained with MBS9217260(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specificprotein-protein interactions followed by the antibody (MBS9217260, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Western Blot
(All lanes : Anti-beta Tubulin at 1:2000 dilution Lane 1: C2C12 whole cell lysateLane 2: L6 whole cell lysateLane 3: mouse brain lysateLane 4: mouse spleen whole cell lysateLane 5: mouse thymus lysateLane 6: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot
(All lanes : Anti-beta Tubulin at 1:2000 dilution
Lane 1: C2C12 whole cell lysateLane 2: L6 whole cell lysateLane 3: mouse brain lysateLane 4: mouse spleen whole cell lysateLane 5: mouse thymus lysateLane 6: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
Uniprot Description
TUBB: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO/C1orf88. Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Belongs to the tubulin family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Cellular Component: cytoplasm; cytoskeleton; microtubule; nuclear envelope lumen; nucleus; protein complex; tubulin complex
Molecular Function: GTP binding; GTPase activating protein binding; GTPase activity; MHC class I protein binding; nucleotide binding; protein complex binding; protein domain specific binding; structural constituent of cytoskeleton; ubiquitin protein ligase binding
Biological Process: cellular process; microtubule-based process; spindle assembly
Research Articles on Tubb5
Similar Products
Product Notes
The Tubb5 tubb5 (Catalog #AAA9217260) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The beta Tubulin reacts with Mouse, Rat; Predicted Reactivity: Bovine, Chicken, Human, Mouse, Pig, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's beta Tubulin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC), Immunohistochemistry (IHC-P). IHC-P~~1:25 FC~~1:25 WB~~1:2000. Researchers should empirically determine the suitability of the Tubb5 tubb5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta Tubulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
