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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
1205 results for "Rat IgG Isotype Control" - showing 50-100
Testing Data
(Figure A. Alexa Fluor 647 conjugated Rat anti Mouse CD122 and FITC conjugated Mouse IgG2a isotype control . Figure B. Alexa Fluor 647 conjugated Rat anti Mouse CD122 and FITC conjugated Mouse anti Mouse CD161 . All experiments performed on mouse blood gated on live, single lymphocytes in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer)
CD161/NK1.1, Monoclonal Antibody (Cat# AAA225624)
Full Name
Mouse Anti Mouse CD161/NK1.1: FITC
Gene Names
Klrb1c; Nk1; Ly59; Nk-1; CD161; Ly-59; Ly55c; NK1.1; NKRP1; Nk1.2; Klrb1b; NK-RP1; Nk-1.2; Nkrp1b; Nkrp1c; ly-55c; NKRP140; AI462337; NKR-P1.9
Reactivity
Mouse
Does not react with: Rat, Human
Does not react with: Rat, Human
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CHRNA5 using anti-CHRNA5 antibody (MBS178666). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat skeletal muscle tissue lysates, lane 2: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHRNA5 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHRNA5 at approximately 53KD. The expected band size for CHRNA5 is at 53KD. )
CHRNA5, Polyclonal Antibody (Cat# AAA178666)
Full Name
Anti-CHRNA5 Antibody
Gene Names
CHRNA5; LNCR2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Skin stained with Nucleolin Monoclonal Antibody (364-5).)
Nucleolin, Monoclonal Antibody (Cat# AAA439209)
Full Name
Nucleolin (Marker of Human Cells) Mouse Monoclonal Antibody
Gene Names
NCL; C23
Reactivity
Does not react with Mouse, Human, Rat and Cow
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Figure 1. IHC analysis of BDNF using anti-BDNF antibody (MBS176974). BDNF was detected in paraffin-embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BDNF Antibody (MBS176974) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
BDNF, Polyclonal Antibody (Cat# AAA176974)
Full Name
Anti-BDNF Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R).)
CD15/FUT4, Monoclonal Antibody (Cat# AAA439984)
Full Name
CD15/FUT4 (Reed-Sternberg Cell Marker)
Gene Names
FUT4; LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV
Reactivity
Human, Mouse, Rat. Others not known.
Applications
FC/FACS, IF, IHC, Formalin-fixed
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Figure 1. Western blot analysis of CD31 using anti-CD31 antibody (MBS176761).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.lane 1: recombinant human CD31 protein 0.5ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD31 at approximately 39KD. The expected band size for CD31 is at 39KD. )
CD31, Polyclonal Antibody (Cat# AAA176761)
Full Name
Anti-CD31 Antibody
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of CX3CR1 in human spleen lysate with CX3CR1 antibody at 1 μg/mL in the absence (lane 1) or presence of blocking peptide (lane 2).)
CX3CR1, Polyclonal Antibody (Cat# AAA150322)
Full Name
CX3CR1 Antibody
Gene Names
CX3CR1; V28; CCRL1; GPR13; CMKDR1; GPRV28; CMKBRL1
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
CX3CR1 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Ataxin 3 using anti-Ataxin 3 antibody (PB9423). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate Lane 2: COLO320 Whole Cell Lysate Lane 3: HELA Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ataxin 3 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ataxin 3 at approximately 42KD. The expected band size for Ataxin 3 is at 42KD.)
Ataxin 3, Polyclonal Antibody (Cat# AAA178269)
Full Name
Anti-Ataxin 3 Antibody
Gene Names
ATXN3; AT3; JOS; MJD; ATX3; MJD1; SCA3
Reactivity
Human, Rat
Applications
WB, IHC, ICC, FC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 8 Monoclonal Antibody (H1).)
Cytokeratin 8 (KRT8), Monoclonal Antibody (Cat# AAA438242)
Full Name
Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody
Gene Names
KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
Reactivity
Human, Rat, Zebrafish
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
(Published customer image: Rat anti Human HLA-DR antibody, clone YE2/36-HLK used for flow cytometryImage caption:BILF1 identified as a lytic gene that downregulates surface MHC class I. 293 (A) or MJS (B) cells were transfected with different EBV genes in the bi-cistronic vector, pCDNA3-IRES-nlsGFP. At 48 hr post-transfection, surface MHC class I was stained with PE-conjugated W6/32 mAb and (in MJS only) MHC class II was stained with PE-conjugated anti-DR mAb, YE2/36-HLK. Two-colour flow cytometry was used to analyse staining in the untransfected GFP- population, shown as the solid line histogram, and in the transfected GFP+ population, shown as the dashed line histogram. The grey histogram denotes background staining obtained with an isotype control PE-conjugated antibody.From: Zuo J, Currin A, Griffin BD, Shannon-Lowe C, Thomas WA, et al. (2009) The Epstein-Barr Virus G-Protein-Coupled Receptor Contributes to Immune Evasion by Targeting MHC Class I Molecules for Degradation. PLoS Pathog 5(1): e1000255.)
HLA DR, Monoclonal Antibody (Cat# AAA211077)
Full Name
RAT ANTI HUMAN HLA DR:FITC
Gene Names
HLA-DQB1; IDDM1; CELIAC1; HLA-DQB
Applications
FC/FACS
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Western Blot (WB)
(Western Blot using MBS624304.)
IRS-1, Polyclonal Antibody (Cat# AAA624304)
Full Name
IRS-1, phosphorylated (Tyr612) (Insulin Receptor Substrate 1)
Gene Names
IRS1; HIRS-1
Reactivity
Mouse
Applications
FC, WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$695/0.1 mL | $3,110/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of MVP using anti-MVP antibody (MBS1750453).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human PANC-1 whole cell lysates,Lane 5: human SGC-7901 whole cell lysates,Lane 6: human MDA-MB-231 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MVP antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MVP at approximately 99KD. The expected band size for MVP is at 99KD. )
MVP, Polyclonal Antibody (Cat# AAA1750453)
Full Name
Anti-MVP Picoband antibody
Gene Names
MVP; LRP; VAULT1
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Flow Cytometry
(Overlay histogram showing Hela cells stained with MBS9207576 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9207576, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was Rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
LPL, Polyclonal Antibody (Cat# AAA9207576)
Full Name
LPL Antibody (Center)
Gene Names
LPL; LIPD; HDLCQ11
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Pig, Rat, Sheep)
Applications
WB, IHC, FC/FACS, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Figure 1. Western blot analysis of Mesothelin using anti-Mesothelin antibody (MBS178354).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: Recombinant Human Mesothelin Protein 0.5ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mesothelin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Mesothelin at approximately 43KD. The expected band size for Mesothelin is at 43KD. )
Mesothelin, Polyclonal Antibody (Cat# AAA178354)
Full Name
Anti-Mesothelin Antibody
Gene Names
MSLN; MPF; SMRP
Reactivity
Human
Applications
WB, IHC, EIA, FC, ICC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA214268)
Full Name
RAT ANTI MOUSE CD8:Low Endotoxin
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Applications
FC/FACS, IF
Pricing
$745/0.5 mg | $3,200/5x0.5 mg
Immunohistochemistry (IHC)
(Formalin-fixed. Paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (S100B/1012).)
S100B, Monoclonal Antibody (Cat# AAA4380220)
Full Name
S100B (Astrocyte and Melanoma Marker)
Gene Names
S100B; NEF; S100; S100-B; S100beta
Reactivity
Human, Mouse, Rat, Cow. Others not known.
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunofluorescence (IF)
( Immunofluorescence staining of fixed mouse splenocytes with anti-CD74 antibody In-1 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of In-1 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD74, Monoclonal Recombinant Antibody (Cat# AAA488365)
Full Name
Anti-CD74 [In-1]
Reactivity
Mouse
Applications
IP, FC/FACS, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human non-Hodgkin's Lymphoma stained with Bcl-2 Monoclonal Antibody (100/D5 + 124). Note nuclear membrane & cytoplasmic staining.)
Bcl-2, Monoclonal Antibody (Cat# AAA438175)
Full Name
Bcl-2 (Apoptosis & Follicular Lymphoma Marker) Mouse Monoclonal Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human.
Does not react with Mouse and Rat
Does not react with Mouse and Rat
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Histone H1 Mouse Recombinant Monoclonal Antibody (rAE-4).)
Histone H1, Antibody (Cat# AAA4380970)
Full Name
Histone H1 (Pan Nuclear Marker)
Gene Names
H1-0; H10; H1.0; H1F0; H1FV
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
(Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and FITC conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.)
CD31, Monoclonal Antibody (Cat# AAA225658)
Full Name
Rat Anti Mouse CD31: FITC
Gene Names
Pecam1; Cd31; Pecam; C85791; PECAM-1
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Flow Cytometry (FC)
(Flow cytometric analysis of U-87 MG cells using RAC1(green) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
RAC1, Monoclonal Antibody (Cat# AAA9200589)
Full Name
RAC1 Antibody
Gene Names
RAC1; MIG5; Rac-1; TC-25; p21-Rac1
Reactivity
Human, rat (Predicted Reactivity: Bovine, Mouse)
Applications
WB, EIA, FC, IHC
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. astroglioma section using NCAM1 Antibody (C-term). MBS9207972 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
NCAM1, Polyclonal Antibody (Cat# AAA9207972)
Full Name
NCAM1 Antibody (C-term)
Gene Names
NCAM1; CD56; NCAM; MSK39
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat)
Applications
FC/FACS, IHC-P, WB, EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
SDS-PAGE
(SDS-PAGE AnalysisCD3e Mouse Monoclonal Antibody (RIV9).Confirmation of Integrity and Purity of Antibody)
CD3, Monoclonal Antibody (Cat# AAA439364)
Full Name
CD3 (T-Cell Marker) Mouse Monoclonal Antibody
Gene Names
CD3E; T3E; TCRE; IMD18
Reactivity
Human, Mouse, Rat
Applications
FC, IF
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Uterus stained with Calponin-1 Recombinant Mouse Monoclonal Antibody (rCNN1/832).)
Calponin-1, Monoclonal Antibody (Cat# AAA439885)
Full Name
Calponin-1 (Smooth Muscle Marker)
Gene Names
CNN1; SMCC; Sm-Calp; HEL-S-14
Reactivity
Human, Rat
Applications
FC, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Figure 1. Western blot analysis of CD8 alpha using anti-CD8 alpha antibody (MBS1750757).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysate,Lane 2: rat thymus tissue lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD8 alpha antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD8 alpha at approximately 36KD. The expected band size for CD8 alpha is at 26KD. )
CD8 alpha, Polyclonal Antibody (Cat# AAA1750757)
Full Name
Anti-CD8 alpha Picoband antibody
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of ADO expression in rat testis extract (lane 1), mouse kidney extract (lane 2) and HELA whole cell lysates (lane 3). ADO at 30KD was detected using rabbit anti- ADO Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
ADO, Polyclonal Antibody (Cat# AAA178788)
Full Name
Anti-ADO Antibody
Gene Names
ADO; C10orf22
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of SH-SY5Y cells using STMN2 Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9214476 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
STMN2, Polyclonal Antibody (Cat# AAA9214476)
Full Name
STMN2 Antibody (N-term)
Gene Names
STMN2; SCG10; SCGN10
Reactivity
Human, Mouse, Rat
Applications
FC, IHC, WB, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data
(Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody , red in A and Mouse anti Rat CD4 , green in B. C is the Merged image with nuclei counter-stained blue using DAPI. High power)
CD172a, Monoclonal Antibody (Cat# AAA210999)
Full Name
MOUSE ANTI RAT CD172a:FITC
Gene Names
Sirpa; Bit; Ptpns1; SHPS-1
Applications
FC/FACS
Pricing
$425/0.1 mg | $1,755/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PTP4A2 using anti-PTP4A2 antibody (MBS177865).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Skeletal Muscle Tissue Lysate,Lane 2: Rat Thymus Tissue Lysate,Lane 3: Mouse Brain Tissue Lysate,Lane 4: Mouse Thymus Tissue Lysate,Lane 5: 22RV1 Whole Cell Lysate,Lane 6: MCF-7 Whole Cell Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTP4A2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTP4A2 at approximately 19KD. The expected band size for PTP4A2 is at 19KD. )
PTP4A2, Polyclonal Antibody (Cat# AAA177865)
Full Name
Anti-PTP4A2 Antibody
Gene Names
PTP4A2; HH13; OV-1; PRL2; HH7-2; PRL-2; PTP4A; HU-PP-1; PTPCAAX2; ptp-IV1a; ptp-IV1b
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, FC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Integrin beta-7 antibody FIB27 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB27 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Integrin beta-7, Monoclonal Recombinant Antibody (Cat# AAA488443)
Full Name
Anti-Integrin beta-7 [FIB27]
Reactivity
Human, Mouse
Applications
FC/FACS, IP, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of SCRIBBLE using anti-SCRIBBLE antibody (MBS1750687).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,Lane 2: human COLO-320 whole cell lysates,Lane 3: human 22RV1 whole cell lysates,Lane 4: human SGC-7901 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCRIBBLE antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SCRIBBLE at approximately 240KD. The expected band size for SCRIBBLE is at 175KD. )
SCRIBBLE, Polyclonal Antibody (Cat# AAA1750687)
Full Name
Anti-SCRIBBLE Picoband antibody
Gene Names
SCRIB; CRIB1; SCRB1; SCRIB1; Vartul
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody , red in A and Mouse anti Rat CD4 , green in B. C is the Merged image with nuclei counter-stained blue using DAPI. High power)
CD172a, Monoclonal Antibody (Cat# AAA212963)
Full Name
MOUSE ANTI RAT CD172a
Gene Names
Sirpa; Bit; Ptpns1; SHPS-1
Applications
FC/FACS
Pricing
$345/0.1 mg | $1,405/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Caspase3 using anti-Caspase3 antibody (MBS177469).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.lane 1: recombinant human Caspase3 protein 0.5ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Caspase3 at approximately 39KD. The expected band size for Caspase3 is at 39KD. )
Caspase-3, Polyclonal Antibody (Cat# AAA177469)
Full Name
Anti-Caspase-3 Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Applications
WB, IHC-P
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse anti Human CD29 . All experiments performed on red cell lysed human blood gated on live platelets, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
CD29, Monoclonal Antibody (Cat# AAA225642)
Full Name
Mouse Anti Human CD29: RPE
Gene Names
ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
Reactivity
Human, Mink
Does not react with: Rat, Mouse
Does not react with: Rat, Mouse
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$500/100 Tests | $2,090/5x100 Tests
Western Blot (WB)
(Figure 1. Western blot analysis of beta Amyloid using anti-beta Amyloid antibody (MBS176795). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human beta-Amyloid protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta Amyloid antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for beta Amyloid at approximately 29KD. The expected band size for beta Amyloid is at 29KD. )
beta Amyloid, Polyclonal Antibody (Cat# AAA176795)
Full Name
Anti-beta Amyloid Antibody
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from A549,SK-BR-3,mouse C2C12 cell line (from left to right), using CAPN2 Antibody (MBS9231851). It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
CAPN2, Monoclonal Antibody (Cat# AAA9231851)
Full Name
CAPN2 Antibody
Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Reactivity
Human, Rat
Predicted: Mouse
Predicted: Mouse
Applications
WB, IHC-P, FC/FACS, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-BRD3 monoclonal antibody. )
BRD3, Monoclonal Antibody (Cat# AAA120113)
Full Name
Mouse monoclonal antibody Anti-Human BRD3
Gene Names
BRD3; ORFX; RING3L
Reactivity
Human
Applications
DB, ICC, IP, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Cyclophilin A using anti- Cyclophilin A antibody (MBS1750616).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat lung tissue lysates,Lane 2: rat brain tissue lysates,Lane 3: mouse lung tissue lysates,Lane 4: HELA whole Cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Cyclophilin A antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cyclophilin A at approximately 18KD. The expected band size for Cyclophilin A is at 18KD. )
Cyclophilin A, Polyclonal Antibody (Cat# AAA1750616)
Full Name
Anti-Cyclophilin A Picoband Antibody
Gene Names
PPIA; CYPA; CYPH; HEL-S-69p
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
FC/FACS, IHC, ICC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(1:2500 dilution of this antibody detected CRTC1 on 40ug of cell lysates)
CRTC1, Monoclonal Antibody (Cat# AAA9400213)
Full Name
CRTC1 Monoclonal Antibody
Gene Names
CRTC1; MECT1; TORC1; TORC-1; WAMTP1
Reactivity
Human, Porcine, Mouse, Rat
Applications
WB, ICC, FC/FACS
Purity
Purified by antigen-affinity chromatography.
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
Testing Data
(Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)
CD18, Monoclonal Antibody (Cat# AAA213058)
Full Name
RAT ANTI HUMAN CD18
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS, IP
Pricing
$340/0.1 mg | $1,375/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CD90/Thy1 using anti- CD90/Thy1 antibody (MBS1750721).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse brain tissue lysates,Lane 2: mouse thymus tissue lysates,Lane 3: mouse lung tissue lysates,Lane 4: rat thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CD90/Thy1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD90/Thy1 at approximately 26KD. The expected band size for CD90/Thy1 is at 18KD. )
CD90/Thy1, Polyclonal Antibody (Cat# AAA1750721)
Full Name
Anti-CD90/Thy1 Picoband Antibody
Gene Names
Thy1; T25; CD90; Thy-1; Thy1.1; Thy1.2; Thy-1.2
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)
CD18, Monoclonal Antibody (Cat# AAA213845)
Full Name
RAT ANTI HUMAN CD18:RPE
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Western Blot (WB)
(Figure 1. Western blot analysis of Synaptotagmin 1 using anti-Synaptotagmin 1 antibody (MBS1750760).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptotagmin 1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Synaptotagmin 1 at approximately 65KD. The expected band size for Synaptotagmin 1 is at 47KD. )
Synaptotagmin 1, Polyclonal Antibody (Cat# AAA1750760)
Full Name
Anti-Synaptotagmin 1 Picoband antibody
Gene Names
SYT1; P65; SYT; SVP65
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PNP using anti-PNP antibody (MBS178628).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat thymus tissue lysates,Lane 2: rat ovary tissue lysates,Lane 3: mouse liver tissue lysates,Lane 4: human placneta tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNP antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PNP at approximately 38KD. The expected band size for PNP is at 38KD. )
PNP, Polyclonal Antibody (Cat# AAA178628)
Full Name
Anti-PNP Antibody
Gene Names
PNP; NP; PUNP; PRO1837
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215797)
Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS, IP
Pricing
$375/0.1 mg | $1,535/5x0.1 mg
Testing Data
(Published customer image:IL-4 DC and IL-10 DC exhibit no obvious differences in their phenotype. IL-4 DC and IL-10 DC and mature splenic DC (sDC) coexpressed Ox62 and CD68, whereas macrophages generated in the presence of M-CSF (5 ug/ml) were only positive for CD68. Broken lines indicate background staining obtained using an irrelevant isotype control. The first number represents the percentage of cells staining positive for the indicated marker and the second number represents the mean fluorescence intensity. The results shown are representative for 4 independent flow cytometric analyses.From:BMC Res Notes. 2009 Jan 23;2:12.)
OX-62, Monoclonal Antibody (Cat# AAA212002)
Full Name
MOUSE ANTI RAT OX-62
Applications
FC/FACS, IP, WB
Pricing
$490/0.25 mg | $2,050/5x0.25 mg
ELISA (EIA)
( Binding curve of anti-ICOSL antibody HK5.3 to mouse ICOSL-Fc fusion protein ELISA Plate coated with ICOSL-Fc fusion protein (Pr00157-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.)
ICOSL, Monoclonal Recombinant Antibody (Cat# AAA488367)
Full Name
Anti-ICOSL [HK5.3]
Gene Names
Icosl; B7h; GI50; GL50; B7-H2; LICOS; B7RP-1; GL50-B; ICOS-L; Icoslg; Ly115l; AU044799; BG071784; mKIAA0653
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ELISA (EIA)
( Binding curve of anti-B7-H3 antibody MJ18 to mouse B7-H3-Fc fusion protein ELISA Plate coated with mouse B7-H3-Fc fusion protein (Pr00158-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.)
B7-H3, Monoclonal Recombinant Antibody (Cat# AAA488371)
Full Name
Anti-B7-H3 [MJ18]
Gene Names
Cd276; B7h3; B7RP-2; AU016588; 6030411F23Rik
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-CD8a antibody YTS 105.18 Mouse lymphocytes were stained with an isotype control or the rabbit-chimeric version of YTS 105.18 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD8 alpha, Recombinant Antibody (Cat# AAA488138)
Full Name
Anti-CD8 alpha [YTS 105.18]
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Reactivity
Mouse
Applications
FC/FACS, IF, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PKC eta using anti-PKC eta antibody (MBS178258). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A431 Whole Cell Lysate Lane 2: A549 Whole Cell Lysate Lane 3: HELA Whole Cell Lysate Lane 4: SKOV Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC eta antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PKC eta at approximately 78KD. The expected band size for PKC eta is at 78KD. )
PKC eta, Polyclonal Antibody (Cat# AAA178258)
Full Name
Anti-PKC eta Antibody
Gene Names
PRKCH; PKCL; PKC-L; PRKCL; nPKC-eta
Reactivity
Human
Applications
WB
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
