Rabbit anti-Human RAPGEF2 Polyclonal Antibody | anti-RAPGEF2 antibody

RAPGEF2 Antibody (C-term)

Cat. Num. AAA9204595

• Gene Names: RAPGEF2; RA-GEF; NRAPGEP; PDZGEF1; Rap-GEP; CNrasGEF; PDZ-GEF1; RA-GEF-1; nRap GEP
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: RAPGEF2; Polyclonal Antibody; RAPGEF2 Antibody (C-term); Rap guanine nucleotide exchange factor 2; Cyclic nucleotide ras GEF; CNrasGEF; Neural RAP guanine nucleotide exchange protein; nRap GEP; PDZ domain-containing guanine nucleotide exchange factor 1; PDZ-GEF1; RA-GEF-1; Ras/Rap1-associating GEF-1; KIAA0313; NRAPGEP; PDZGEF1; anti-RAPGEF2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RAPGEF2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1464-1498 amino acids from the C-terminal region of human RAPGEF2.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 1,499

• Applicable Applications for anti-RAPGEF2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:2000
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-RAPGEF2 Antibody (C-term) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: HT-1080 whole cell lysatesLane 3: 293T/17 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 167 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-RAPGEF2 antibody

Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP (PubMed:23800469, PubMed:10801446) or not (PubMed:10608844, PubMed:10548487, PubMed:11359771). Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP- induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain- derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions.

Product Categories/Family for anti-RAPGEF2 antibody

Neuroscience; Signal Transduction

References

Nagase T.,et al.DNA Res. 4:141-150(1997).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Ohtsuka T.,et al.Biochem. Biophys. Res. Commun. 265:38-44(1999).
Liao Y.,et al.J. Biol. Chem. 274:37815-37820(1999).
de Rooij J.,et al.J. Biol. Chem. 274:38125-38130(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 7657261
• NCBI GeneID: 9693
• NCBI Accession #: NP_055062.1
• NCBI GenBank Nucleotide #: NM_014247.2
• UniProt Accession #: Q9Y4G8; D3DP27
• Molecular Weight: 167417
• NCBI Official Full Name: rap guanine nucleotide exchange factor 2
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor (GEF) 2
• NCBI Official Symbol: RAPGEF2
• NCBI Official Synonym Symbols: RA-GEF; NRAPGEP; PDZGEF1; Rap-GEP; CNrasGEF; PDZ-GEF1; RA-GEF-1; nRap GEP
• NCBI Protein Information: rap guanine nucleotide exchange factor 2
• UniProt Protein Name: Rap guanine nucleotide exchange factor 2
• Protein Family: Rap guanine nucleotide exchange factor
• UniProt Gene Name: RAPGEF2
• UniProt Synonym Gene Names: KIAA0313; NRAPGEP; PDZGEF1; CNrasGEF; nRap GEP; PDZ-GEF1
• UniProt Entry Name: RPGF2_HUMAN

NCBI Description

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008]

Uniprot Description

RAPGEF2: Guanine nucleotide exchange factor (GEF) for Rap1A, Rap1B and Rap2B GTPases. Does not interact with cAMP or cGMP. Interacts with MAGI2. Highest expression levels in brain. Lower expression levels in heart, kidney, lung, and placenta.

Protein type: Motility/polarity/chemotaxis; GEFs; GEFs, Ras

Chromosomal Location of Human Ortholog: 4q32.1

Cellular Component: neuron projection; tight junction; protein complex; integral to plasma membrane; intercellular junction; cytosol; membrane; cell soma; perinuclear region of cytoplasm; endocytic vesicle; late endosome; apical plasma membrane; cytoplasm; plasma membrane; synapse

Molecular Function: diacylglycerol binding; protein binding; signal transducer activity; Rap guanyl-nucleotide exchange factor activity; beta-1 adrenergic receptor binding; WW domain binding; cGMP binding; calcium ion binding; cAMP binding; protein kinase binding; Ras guanyl-nucleotide exchange factor activity; PDZ domain binding

Biological Process: blood vessel development; positive regulation of protein binding; brain-derived neurotrophic factor receptor signaling pathway; ventricular system development; neuron migration; MAPKKK cascade; regulation of synaptic plasticity; negative regulation of dendrite morphogenesis; negative regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; microvillus biogenesis; cAMP-mediated signaling; forebrain neuron development; neuropeptide signaling pathway; positive regulation of protein kinase activity; small GTPase mediated signal transduction; negative regulation of melanin biosynthetic process; Rap protein signal transduction; neurite development