Western Blot (WB)
(Western Blot detection of human FOLR1 using MBS605669. Lysates were prepared from Hela cells in non-reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with MBS605669 (2ug/ml) and chemiluminescent detection substrate.)
Mouse anti-Human FOLR1 Monoclonal Antibody | anti-FOLR1 antibody
FOLR1 (Folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FOLR, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18)
ELISA (Capture): 2-8ug/ml
Flow Cytometry: 0.25ug labels/10e6 cells. Tested in MCF-7 cells.
Western Blot: 2ug/ml detects human FOLR1 in human cortex tissue and HeLa cells under non-reducing conditions only.
Immunocytochemistry: 8-25ug/ml. Tested on immersion fixed MCF-7 cells.
Optimal dilutions to be determined by the researcher.
Western Blot (WB)
(Western Blot detection of human FOLR1 using MBS605669. Lysates were prepared from Hela cells in non-reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with MBS605669 (2ug/ml) and chemiluminescent detection substrate.)
Western Blot (WB)
(Western Blot detection of human FOLR1 using MBS605669. Lysates were prepared from Hela cells in non-reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with MBS605669 (2ug/ml) and chemiluminescent detection substrate.)
Testing Data
(Detection of FOLR1 in MCF-7 human cell line. FOLR1 was detected in immersion fixed MCF-7 human breast cancer cell line using MBS605669 (10ug/ml) for 3 hours at RT. Cells were stained with the DayLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and|counterstained with DAPI (blue).)
Testing Data
(Detection of FOLR1 in MCF-7 human cell line. FOLR1 was detected in immersion fixed MCF-7 human breast cancer cell line using MBS605669 (10ug/ml) for 3 hours at RT. Cells were stained with the DayLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and|counterstained with DAPI (blue).)
Flow Cytometry (FC/FACS)
(Flow Cytometry detection of FOLR1 in MCF-7 human cell line using MBS605669. MCF-7 human breast cancer cell line was stained with MBS605669 (filled histogram) or isotype control antibody (open histogram), followed by|Phycoerythrin-conjugated Anti-Mouse|IgG Secondary Antibody.)
Flow Cytometry (FC/FACS)
(Flow Cytometry detection of FOLR1 in MCF-7 human cell line using MBS605669. MCF-7 human breast cancer cell line was stained with MBS605669 (filled histogram) or isotype control antibody (open histogram), followed by|Phycoerythrin-conjugated Anti-Mouse|IgG Secondary Antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2009]
Uniprot Description
FOLR1: Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain- specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function. Belongs to the folate receptor family.
Protein type: Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 11q13.3-q14.1
Cellular Component: anchored to external side of plasma membrane; cell surface; clathrin-coated vesicle; membrane; brush border membrane; integral to plasma membrane; apical plasma membrane; plasma membrane; endosome
Molecular Function: folic acid transporter activity; receptor activity; folic acid binding
Biological Process: receptor-mediated endocytosis; folic acid metabolic process; folic acid transport
Disease: Neurodegeneration Due To Cerebral Folate Transport Deficiency
Research Articles on FOLR1
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Product Notes
The FOLR1 folr1 (Catalog #AAA605669) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FOLR1 (Folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FOLR, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FOLR1 can be used in a range of immunoassay formats including, but not limited to, Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA (Capture): 2-8ug/ml Flow Cytometry: 0.25ug labels/10e6 cells. Tested in MCF-7 cells. Western Blot: 2ug/ml detects human FOLR1 in human cortex tissue and HeLa cells under non-reducing conditions only. Immunocytochemistry: 8-25ug/ml. Tested on immersion fixed MCF-7 cells. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the FOLR1 folr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOLR1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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