Mouse CD68 Antibody | anti-CD68 antibody

CD68 (Macrophage Marker) Mouse Monoclonal Antibody

Cat. Num. AAA438131

• Gene Names: CD68; GP110; LAMP4; SCARD1
• Reactivity: Human, Monkey, Mouse, and Rat. Others not known.
• Applications: Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry
• Synonyms: CD68; CD68 (Macrophage Marker) Mouse Monoclonal Antibody; GP110; LAMP4; Microsialin; Macrosialin; SCARD1; Scavenger Receptor Class D Member-1; anti-CD68 antibody

• Host: Mouse
• Reactivity: Human, Monkey, Mouse, and Rat. Others not known.
• Isotype: IgG1, kappa
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

• Applicable Applications for anti-CD68 antibody: Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC), Immunohistology (Formalin-fixed)
• Application Notes: Western blotting (1-2ug/ml); Immunofluorescence (1-2ug/ml); Flow Cytometry (1-2ug/million cells); Immunochemistry (Formalin-fixed) (1-2 ug/ml for 30 minutes at RT) ; (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); ; Optimal dilution for a specific application should be determined.
• Human Unigene: 647419
• Hu-Chromosome Location: 17p13.1
• Immunogen: Recombinant human CD68 protein
• Cellular Localization: Cell Surface and Cytoplasmic
• Positive Control: U87MG cells,Tonsil, Lymph Node or Spleen
• Preparation and Storage: Antibody with azide - store at 2 to 8°C. Non-hazardous. No MSDS required

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68 Monoclonal Antibody (C68/684).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Tonsil stained with CD68 Monoclonal Antibody (C68/684).)

SDS-PAGE

(SDS-PAGE Analysis of Purified CD68 Monoclonal Antibody (C68/684).)

Western Blot (WB)

(Western Blot Analysis of human Spleen tissue lysate using CD68 Monoclonal Antibody (C68/684).)

Flow Cytometry (FC)

( Flow Cytometric Analysis of U87MG cells using CD68 Monoclonal Antibody (C68/684); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).)

Immunofluorescence

(Immunofluorescence staining of U87MG cells using CD68 Mouse Monoclonal Antibody (C68/684) followed by goat anti-Mouse IgG conjugated to MBS4380777 (green). Nuclei are stained with Red dot)

Related Product Information for anti-CD68 antibody

This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

References

Pulford KA et. al. Journal of Clinical Pathology, 1989, 42(4):414-21. Warnke RA et. al. Am J of Pathol, 1989, 135:1089-95.

NCBI and Uniprot Product Information

• NCBI GI #: 91199550
• NCBI GeneID: 968
• NCBI Accession #: NP_001035148.1
• NCBI GenBank Nucleotide #: NM_001040059.1
• UniProt Accession #: P34810; Q53HR6; Q53XI3; Q96BI7; B4DVT4
• Molecular Weight: 110kDa
• NCBI Official Full Name: macrosialin isoform B
• NCBI Official Synonym Full Names: CD68 molecule
• NCBI Official Symbol: CD68
• NCBI Official Synonym Symbols: GP110; LAMP4; SCARD1
• NCBI Protein Information: macrosialin
• UniProt Protein Name: Macrosialin
• Protein Family: Macrosialin
• UniProt Gene Name: CD68
• UniProt Entry Name: CD68_HUMAN

NCBI Description

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: membrane