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Quality Control (Western blot analysis of immunized recombinant protein, using anti-ABCF2 monoclonal antibody. )

Mouse anti-Human ABCF2 Monoclonal Antibody | anti-ABCF2 antibody

Mouse monoclonal antibody Anti-Human ABCF2

Gene Names
ABCF2; ABC28; HUSSY18; HUSSY-18; EST133090
Reactivity
Human
Applications
Dot Blot, Immunoprecipitation, Western Blot, Flow Cytometry, Functional Assay
Synonyms
ABCF2; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human ABCF2; Homo sapiens ATP-binding cassette; sub-family F (GCN20); member 2 (ABCF2); nuclear gene encoding mitochondrial protein; transcript variant 2; mRNA; ABC28; M-ABC1; HUSSY-18; EST133090;DKFZp586K1823; anti-ABCF2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2001C1
Concentration
100 ug/ml (1.0 ml) (varies by lot)
Sequence Length
2405
Applicable Applications for anti-ABCF2 antibody
Dot Blot (DB), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-ABCF2 monoclonal antibody. )

Quality Control (Western blot analysis of immunized recombinant protein, using anti-ABCF2 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Western Blot (WB)

(Detection of ABCF2 by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 2001C1-1]Predicted molecular weight: 71 kDa)

Western Blot (WB) (Detection of ABCF2 by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 2001C1-1]Predicted molecular weight: 71 kDa)

Immunoprecipitation (IP)

(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-ABCF2 mAb. [Lot No. 2001C1-1]Predicted molecular weight: 71 kDa)

Immunoprecipitation (IP) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-ABCF2 mAb. [Lot No. 2001C1-1]Predicted molecular weight: 71 kDa)

Flow Cytometry (FC/FACS)

(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-ABCF2 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2001C1-1])

Flow Cytometry (FC/FACS) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-ABCF2 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2001C1-1])
Related Product Information for anti-ABCF2 antibody
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). This protein is a member of the GCN20 subfamily. Alternative splicing of this gene results in multiple transcript variants. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,444 Da
NCBI Official Full Name
Homo sapiens ATP-binding cassette, sub-family F (GCN20), member 2 (ABCF2), transcript variant 2, mRNA
NCBI Official Synonym Full Names
ATP-binding cassette, sub-family F (GCN20), member 2
NCBI Official Symbol
ABCF2
NCBI Official Synonym Symbols
ABC28; HUSSY18; HUSSY-18; EST133090
NCBI Protein Information
ATP-binding cassette sub-family F member 2; ABC-type transport protein; iron-inhibited ABC transporter 2
UniProt Protein Name
ATP-binding cassette sub-family F member 2
Protein Family
UniProt Gene Name
ABCF2
UniProt Entry Name
ABCF2_HUMAN

NCBI Description

This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ATP-binding casette proteins transport various molecules across extra- and intracellular membranes. Alterations in this gene may be involved in cancer progression. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 7. [provided by RefSeq, Jul 2013]

Uniprot Description

ABCF2: is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). This protein is a member of the GCN20 subfamily. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: mitochondrial envelope; membrane; ATP-binding cassette (ABC) transporter complex

Molecular Function: transporter activity; ATPase activity; ATP binding

Biological Process: transport; metabolic process

Research Articles on ABCF2

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Product Notes

The ABCF2 abcf2 (Catalog #AAA120190) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human ABCF2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ABCF2 can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the ABCF2 abcf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ABCF2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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