Western Blot (WB)
(Figure 1. Western blot analysis of CYP1A1 using anti-CYP1A1 antibody (MBS178240).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Lung Tissue LysateLane 2: Mouse Lung Tissue LysateLane 3: Human Placenta Tissue LysateLane 4: JURKAT Whole Cell LysateAfter Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP1A1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYP1A1 at approximately 58KD. The expected band size for CYP1A1 is at 58KD. )
CYP1A1 Polyclonal Antibody | anti-CYP1A1 antibody
Anti-CYP1A1 Antibody
IHC-P: Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat
IHC-F: Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse
ICC/IF: Concentration: 2 ug/ml; Tested Species: Human
FC: Concentration: 1-3ug/1x106 cells; Tested Species: Human.
Tested Species: In house tested species with positive results.
By heat: Boiling the paraffin section 10mM citrate buffer, pH6.0 for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end user.
Western Blot (WB)
(Figure 1. Western blot analysis of CYP1A1 using anti-CYP1A1 antibody (MBS178240).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Lung Tissue LysateLane 2: Mouse Lung Tissue LysateLane 3: Human Placenta Tissue LysateLane 4: JURKAT Whole Cell LysateAfter Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP1A1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYP1A1 at approximately 58KD. The expected band size for CYP1A1 is at 58KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of CYP1A1 using anti-CYP1A1 antibody (MBS178240).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Lung Tissue LysateLane 2: Mouse Lung Tissue LysateLane 3: Human Placenta Tissue LysateLane 4: JURKAT Whole Cell LysateAfter Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP1A1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYP1A1 at approximately 58KD. The expected band size for CYP1A1 is at 58KD. )
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of CYP1A1 using anti-CYP1A1 antibody (MBS178240).CYP1A1 was detected in paraffin-embedded section of Mouse Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CYP1A1 Antibody (MBS178240) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of CYP1A1 using anti-CYP1A1 antibody (MBS178240).CYP1A1 was detected in paraffin-embedded section of Mouse Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CYP1A1 Antibody (MBS178240) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 3. IHC analysis of CYP1A1 using anti-CYP1A1 antibody (MBS178240).CYP1A1 was detected in paraffin-embedded section of Rat Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CYP1A1 Antibody (MBS178240) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 3. IHC analysis of CYP1A1 using anti-CYP1A1 antibody (MBS178240).CYP1A1 was detected in paraffin-embedded section of Rat Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CYP1A1 Antibody (MBS178240) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of CYP1A1 using anti-CYP1A1 antibody (MBS178240).CYP1A1 was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CYP1A1 Antibody (MBS178240) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of CYP1A1 using anti-CYP1A1 antibody (MBS178240).CYP1A1 was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CYP1A1 Antibody (MBS178240) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 5. IHC analysis of CYP1A1 using anti-CYP1A1 antibody (MBS178240).CYP1A1 was detected in frozen section of Mouse Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CYP1A1 Antibody (MBS178240) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 5. IHC analysis of CYP1A1 using anti-CYP1A1 antibody (MBS178240).CYP1A1 was detected in frozen section of Mouse Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CYP1A1 Antibody (MBS178240) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 6. IHC analysis of CYP1A1 using anti-CYP1A1 antibody (MBS178240).CYP1A1 was detected in frozen section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CYP1A1 Antibody (MBS178240) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 6. IHC analysis of CYP1A1 using anti-CYP1A1 antibody (MBS178240).CYP1A1 was detected in frozen section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CYP1A1 Antibody (MBS178240) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Flow Cytometry (FC/FACS)
(Figure 7. Flow Cytometry analysis of CACO-2 cells using anti-CYP1A1 antibody (MBS178240).Overlay histogram showing CACO-2 cells stained with MBS178240 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP1A1 Antibody (MBS178240,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 7. Flow Cytometry analysis of CACO-2 cells using anti-CYP1A1 antibody (MBS178240).Overlay histogram showing CACO-2 cells stained with MBS178240 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP1A1 Antibody (MBS178240,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 8. Flow Cytometry analysis of Hela cells using anti-CYP1A1 antibody (MBS178240).Overlay histogram showing Hela cells stained with MBS178240 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP1A1 Antibody (MBS178240,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 8. Flow Cytometry analysis of Hela cells using anti-CYP1A1 antibody (MBS178240).Overlay histogram showing Hela cells stained with MBS178240 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP1A1 Antibody (MBS178240,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 9. Flow Cytometry analysis of K562 cells using anti-CYP1A1 antibody (MBS178240).Overlay histogram showing K562 cells stained with MBS178240 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP1A1 Antibody (MBS178240,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 9. Flow Cytometry analysis of K562 cells using anti-CYP1A1 antibody (MBS178240).Overlay histogram showing K562 cells stained with MBS178240 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP1A1 Antibody (MBS178240,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
NCBI and Uniprot Product Information
NCBI Description
This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2016]
Uniprot Description
CYP1A1: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Belongs to the cytochrome P450 family.
Protein type: Oxidoreductase; Cofactor and Vitamin Metabolism - retinol; Amino Acid Metabolism - tryptophan; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.14.14.1
Chromosomal Location of Human Ortholog: 15q24.1
Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; mitochondrion
Molecular Function: demethylase activity; enzyme binding; flavonoid 3'-monooxygenase activity; heme binding; iron ion binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on diphenols and related substances as donors; oxygen binding; protein binding; steroid hydroxylase activity
Biological Process: 9-cis-retinoic acid biosynthetic process; aging; amine metabolic process; camera-type eye development; cell proliferation; coumarin metabolic process; dibenzo-p-dioxin catabolic process; drug metabolic process; embryonic development ending in birth or egg hatching; epoxygenase P450 pathway; ethylene metabolic process; flavonoid metabolic process; gut development; hydrogen peroxide biosynthetic process; insecticide metabolic process; maternal process involved in parturition; porphyrin metabolic process; response to antibiotic; response to arsenic; response to drug; response to food; response to herbicide; response to hyperoxia; response to hypoxia; response to iron(III) ion; response to lipopolysaccharide; response to nematode; response to virus; response to vitamin A; response to wounding; vitamin D metabolic process
Research Articles on CYP1A1
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Product Notes
The CYP1A1 cyp1a1 (Catalog #AAA178240) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CYP1A1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CYP1A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunocytochemistry (ICC), Flow Cytometry (FC). WB: Concentration: 0.1-0.5ug/ml; Tested Species: Human Mouse, Rat IHC-P: Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat IHC-F: Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse ICC/IF: Concentration: 2 ug/ml; Tested Species: Human FC: Concentration: 1-3ug/1x106 cells; Tested Species: Human. Tested Species: In house tested species with positive results. By heat: Boiling the paraffin section 10mM citrate buffer, pH6.0 for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end user. Researchers should empirically determine the suitability of the CYP1A1 cyp1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYP1A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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