Mouse CD86 Monoclonal Antibody | anti-CD86 antibody

CD86 (Dendritic Cells Maturation Marker) Mouse Monoclonal Antibody

Cat. Num. AAA438121

• Gene Names: CD86; B70; B7-2; B7.2; LAB72; CD28LG2
• Reactivity: Human, Mouse, Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry
• Synonyms: CD86; Monoclonal Antibody; CD86 (Dendritic Cells Maturation Marker) Mouse Monoclonal Antibody; Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD28LG2; CLS1; CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly-58; MB7-2; TS/A-2; anti-CD86 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: BU63
• Specificity: Recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 217

• Applicable Applications for anti-CD86 antibody: Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) Formalin
• Application Notes: Functional Studies (Order Ab without Azide); Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); ; Optimal dilution for a specific application should be determined.
• Cellular Localization: Cell Surface
• Immunogen: ARH-77 (B-lymphoblastoid cell line)
• Hu-Chromosome Location: 3q13.33
• Human Unigene: 171182
• Positive Control: 293T or Jukat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.
• Preparation and Storage: Antibody with azide - store at 2-8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Esophageal Tumor stained with CD86 Monoclonal Antibody (BU63).)

Flow Cytometry (FC/FACS

(Flow Cytometric Analysis of human PBMC's Cells. usingCD86 Mouse Monoclonal Antibody (BU63); Goat anti-mouse IgG-CF488 (red); Isotype Control (green).)

Flow Cytometry (FC/FACS)

(Flow Cytometric Analysis of PFA fixed Ramos Cells. CD86 Mouse Monoclonal Antibody (BU63). followed byGoat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).)

Immunofluorescence (IF)

(mmunofluorescencestaining of PFA fixed Ramos cells using CD86 Mouse Monoclonal Antibody (BU63) followed by goat anti-mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.)

SDS-PAGE

(SDS-PAGE Analysis Purified CD86 Mouse Monoclonal Antibody (BU63) Confirmation of Integrity and Purity of Antibody.)

References

Engel P et. al. Blood. 1994;84(5):1402-7.Caux C et. al. J Exp Med. 1994;180(5):1841-7. Mauri D et. al. J Immunol. 1995;155(1):118-27.Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)

NCBI and Uniprot Product Information

• NCBI GI #: 332634950
• NCBI GeneID: 942
• NCBI Accession #: NP_001193853.1
• NCBI GenBank Nucleotide #: NM_001206924.1
• UniProt Accession #: P42081; Q13655; Q6FHB1; Q6GTS4; Q7M4L5; A0N0P0; B7Z2F3; B7Z702; E7ETN5; E9PC27
• Molecular Weight: 70kDa
• NCBI Official Full Name: T-lymphocyte activation antigen CD86 isoform 4
• NCBI Official Synonym Full Names: CD86 molecule
• NCBI Official Symbol: CD86
• NCBI Official Synonym Symbols: B70; B7-2; B7.2; LAB72; CD28LG2
• NCBI Protein Information: T-lymphocyte activation antigen CD86
• UniProt Protein Name: T-lymphocyte activation antigen CD86
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: CD86
• UniProt Synonym Gene Names: CD28LG2
• UniProt Entry Name: CD86_HUMAN

NCBI Description

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Uniprot Description

CD86: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: cell surface; integral to membrane; plasma membrane

Molecular Function: phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; receptor activity

Biological Process: cell-cell signaling; immune response; phosphoinositide-mediated signaling; positive regulation of cell proliferation; regulation of phosphoinositide 3-kinase cascade; T cell activation; T cell costimulation