Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
1203 results for " neutrophils" - showing 250-300
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216592)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN
Applications
FC/FACS, IF, WB
Pricing
$210/0.025 mg | $800/5x0.025 mg
Midkine, Active Protein (Cat# AAA691986)
Full Name
Mouse Midkine
Gene Names
Mdk; MK; Mek
Reactivity
Mouse
Purity
> 98% by SDS-PAGE & HPLC analyses
Pricing
$395/0.02 mg | $250/0.005 mg | $1,565/5x0.02 mg
SDS-Page
(Recombinant protein Human CXCL1/GRO was determined by SDS-PAGE under reducing conditions with Coomassie Blue, showing a band at 10 kDa.)
CXCL1/GRO, Active Protein (Cat# AAA9139778)
Full Name
Recombinant Human CXCL1/GRO Protein
Gene Names
CXCL1; FSP; GRO1; GROa; MGSA; NAP-3; SCYB1; MGSA-a
Purity
>95% by SDS-PAGE.
Pricing
$160/0.01 mg | $260/0.05 mg | $1,370/0.5 mg | $1,860/1 mg | $8,315/5x1 mg
Midkine, Active Protein (Cat# AAA145112)
Full Name
Recombinant Mouse Midkine
Gene Names
Mdk; MK; Mek
Purity
Greater than 95.0% as determined by HPLC and SDS-PAGE.
Pricing
$250/0.02 mg | $2,830/1 mg | $180/0.005 mg | $12,520/5x1 mg
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FCGR2A protein (Right) or empty vector (Left) detected with FCGR2A antibody)
FCGR2A, Monoclonal Antibody (Cat# AAA533439)
Full Name
FCGR2A antibody
Applications
IF, IHC, WB
Purity
FCGR2A antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of HL60, using FGR antibody. The lane on the left is treated with the antigen-specific peptide.)
FGR, Polyclonal Antibody (Cat# AAA9607459)
Full Name
FGR Antibody
Gene Names
FGR; SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr
Reactivity
Human
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of K562 cell lysate, using S100A12 Antibody. The lane on the left is treated with the antigen-specific peptide.)
S100A12, Polyclonal Antibody (Cat# AAA9607915)
Full Name
S100A12 Antibody
Gene Names
S100A12; p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215798)
Full Name
RAT ANTI MOUSE DECTIN-1:RPE
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$550/100 Tests | $2,320/5x100 Tests
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216595)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
FC/FACS
Pricing
$255/25 Tests | $995/5x25 Tests
GRO/KC, Rat, Active Protein (Cat# AAA651024)
Full Name
GRO/KC, Recombinant, Rat (Cxcl1, Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, alpha), Cinc1, CINC-1, C-X-C Motif Chemokine 1, Cytokine-induced Neutrophil Chemoattractant 1, Gro1, Growth-regulated alpha Protein, Platelet-derived
Gene Names
Cxcl1; Gro1; CINC-1
Purity
Molecular Biology Grade
98% by SDS-PAGE and HPLC
98% by SDS-PAGE and HPLC
Pricing
$480/0.025 mg | $330/0.005 mg | $2,150/5x0.025 mg
Flow Cytometry (FC/FACS)
(Human peripheral blood neutrophils are stained with Anti-Human CD15 Monoclonal Antibody(APC Conjugated)(filled gray histogram). Unstained neutrophils (empty black histogram) are used as control.)
CD15, Monoclonal Antibody (Cat# AAA2556511)
Full Name
Anti-Human CD15 Monoclonal Antibody (APC Conjugated)
Gene Names
FUT4; LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV
Reactivity
Human
Applications
FC/FACS
Pricing
$215/100 Tests | $280/200 Tests | $165/50 Tests | $130/20 Tests | $1,255/5x200 Tests
MIP-2 (CXCL2, Gro-beta), Active Protein (Cat# AAA691971)
Full Name
Mouse MIP-2 (CXCL2, Gro-beta)
Gene Names
Cxcl2; GROb; Gro2; Mip2; Scyb; MIP-2; Scyb2; MIP-2a; Mgsa-b; CINC-2a
Reactivity
Mouse
Purity
> 98% (SDS-PAGE; HPLC)
Pricing
$395/0.02 mg | $250/0.005 mg | $1,565/5x0.02 mg
Western Blot
(Western Blot analysis using Proteinase 3 antibodyWestern Blot showing PRTN3 antibody used against PRTN3 (aa28-256)-hIgGFc transfected HEK293 cell lysate (1).)
Proteinase 3, Monoclonal Antibody (Cat# AAA831940)
Full Name
Proteinase 3 antibody
Gene Names
PRTN3; MBN; MBT; NP4; P29; PR3; ACPA; AGP7; NP-4; PR-3; CANCA; C-ANCA
Applications
EIA, WB
Purity
Proteinase 3 antibody was purified by protein G affinity chromatography.
Pricing
$605/0.1 mL | $2,715/5x0.1 mL
Interleukin 8, 72aa, Active Protein (Cat# AAA650913)
Full Name
Interleukin 8, Recombinant, Human, 72aa (IL-8)
Gene Names
IL8; NAF; GCP1; LECT; LUCT; NAP1; CXCL8; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
Purity
Highly Purified
98% by SDS-PAGE and HPLC Endotoxin: 0.1ng/ug
98% by SDS-PAGE and HPLC Endotoxin: 0.1ng/ug
Pricing
$480/0.025 mg | $330/0.005 mg | $2,150/5x0.025 mg
GRO gamma, Active Protein (Cat# AAA650677)
Full Name
GRO gamma, Recombinant, Human
Purity
Molecular Biology Grade
98% by SDS-PAGE and HPLC analyses.
98% by SDS-PAGE and HPLC analyses.
Pricing
$480/0.01 mg | $330/0.002 mg | $2,150/5x0.01 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215793)
Full Name
RAT ANTI MOUSE DECTIN-1:FITC
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$460/0.1 mg | $1,910/5x0.1 mg
Testing Data
(Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)
CD18, Monoclonal Antibody (Cat# AAA211045)
Full Name
RAT ANTI HUMAN CD18:FITC
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS
Pricing
$415/100 Tests | $1,710/5x100 Tests
Interleukin-8, Active Protein (Cat# AAA144659)
Full Name
Recombinant Rhesus Macaque Interleukin-8
Gene Names
CXCL8; IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
Purity
Greater than 97.0% as determined by SDS-PAGE.
Pricing
$2,830/1 mg | $250/0.025 mg | $180/0.005 mg | $12,520/5x1 mg
ENA-78 (8-78aa) / CXCL5), Active Protein (Cat# AAA691659)
Full Name
Human ENA-78 (8-78aa) / CXCL5)
Gene Names
CXCL5; SCYB5; ENA-78
Reactivity
Human
Purity
98 % (SDS-PAGE, HPLC)
Pricing
$395/0.02 mg | $250/0.005 mg | $1,565/5x0.02 mg
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FCGR2A protein (Right) or empty vector (Left) detected with FCGR2A antibody)
FCGR2A, Monoclonal Antibody (Cat# AAA831512)
Full Name
FCGR2A antibody
Applications
IF, IHC, WB
Purity
FCGR2A antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FCGR2A protein (Right) or empty vector (Left) detected with FCGR2A antibody)
FCGR2A, Monoclonal Antibody (Cat# AAA831878)
Full Name
FCGR2A antibody
Applications
FC/FACS, IF, IHC, WB
Purity
FCGR2A antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Interleukin-8, Active Protein (Cat# AAA143274)
Full Name
Recombinant Human Interleukin-8 (1-77) (CXCL8) Pichia
Gene Names
CXCL8; IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
Purity
Greater than 98.0% as determined by SDS-PAGE.
Pricing
$2,830/1 mg | $250/0.025 mg | $180/0.005 mg | $12,520/5x1 mg
GRO beta, Rat, Active Protein (Cat# AAA650180)
Full Name
GRO beta, Recombinant, Rat
Purity
Molecular Biology Grade
Greater than 98% by SDS-PAGE and HPLC analyses.
Greater than 98% by SDS-PAGE and HPLC analyses.
Pricing
$480/0.025 mg | $330/0.005 mg | $2,150/5x0.025 mg
Western Blot (WB)
(Western blot analysis of HCK in lysates of Jurkat, using HCK Antibody.)
HCK, Polyclonal Antibody (Cat# AAA9610999)
Full Name
HCK Antibody
Gene Names
HCK; JTK9; p59Hck; p61Hck
Reactivity
Human
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$245/0.05 mL | $305/0.1 mL | $365/0.2 mL | $1,495/5x0.2 mL
MDK, Active Protein (Cat# AAA553656)
Full Name
MDK
Gene Names
MDK; MK; ARAP; NEGF2
Applications
SDS-PAGE
Purity
>95%, as determined by SDS-PAGE and HPLC.
Pricing
$290/0.02 mg | $705/0.1 mg | $3,285/1 mg
Western Blot (WB)
(Western blot analysis of CD32 expression in K562 cell lysate (MBS179660).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCGR2A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FCGR2A )
CD32, Monoclonal Antibody (Cat# AAA179660)
Full Name
Anti-CD32 Rabbit Monoclonal Antibody
Gene Names
FCGR2A; CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
Reactivity
Human
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Testing Data
(Texas red conjugated Goat anti Mouse IgG antibody used for the detection of bound anti CD80 antibody by immunofluorescenceImage caption:Immunolabelling of Mphi by the monocyte/Mphi marker CD14 and the M1 Mphi marker CD80. Mphi were seeded at a density of 5.7×104 cells/cm2, with (coculture) or without the same number of MDA-MB-231 cells. In each case, the culture medium was replaced daily. 5 days later, all cultures were treated with 1 mug/ml LPS and 5 ng/ml IFNgamma for 24 h before washing and labelling. CD14 fluorescent labelling is green, CD80 is red. Panel A: without TM, it can be seen that CD80 was expressed on activated Mphi cultured alone and was not detectable on Mphi in the cocultures. Panel B: in presence of TM, no CD80-positive cells were found in any of the cultures.From: Joimel U, Gest C, Soria J, Pritchard LL, Alexandre J, Laurent M, Blot E, Cazin L, Vannier JP, Varin R, Li H, Soria C.Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate.BMC Cancer. 2010 Jul 17;10:375)
IgG, Polyclonal Antibody (Cat# AAA225563)
Full Name
Goat Anti Mouse IgG: FITC
Reactivity
Mouse
Applications
FC/FACS, IF
Purity
Purified Ig prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose
Pricing
$250/1 mg | $975/5x1 mg
Western Blot (WB)
(Western blot analysis of extracts from Hela cells, using Syntaxin4 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 40 kDa.)
Syntaxin4, Polyclonal Antibody (Cat# AAA9611512)
Full Name
Syntaxin4 Antibody
Gene Names
STX4; STX4A; p35-2
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Applications
WB
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Phospho-HCK (Tyr522) in lysates of Jurkat, using Phospho-HCK (Tyr522) Antibody.)
HCK, Polyclonal Antibody (Cat# AAA9610573)
Full Name
Phospho-HCK (Tyr522) Antibody
Gene Names
HCK; JTK9; p59Hck; p61Hck
Reactivity
Human
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Applications
WB, EIA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Epithelial Neutrophil-Activating Protein 78, Active Protein (Cat# AAA143795)
Full Name
Recombinant Mouse Epithelial Neutrophil-Activating Protein 78 (CXCL5)
Gene Names
Cxcl5; LIX; GCP-2; Scyb5; Scyb6; ENA-78; AMCF-II
Purity
Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
$250/0.02 mg | $2,830/1 mg | $180/0.005 mg | $12,520/5x1 mg
IL-8 (72aa) (CXCL8), Active Protein (Cat# AAA692017)
Full Name
Human IL-8 (72aa) (CXCL8)
Gene Names
IL8; NAF; GCP1; LECT; LUCT; NAP1; CXCL8; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
Reactivity
Human
Purity
> 98% by SDS-PAGE and HPLC analysis
Pricing
$395/0.025 mg | $250/0.005 mg | $1,565/5x0.025 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215799)
Full Name
RAT ANTI MOUSE DECTIN-1:RPE
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$255/25 Tests | $995/5x25 Tests
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216593)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
FC/FACS
Pricing
$550/100 Tests | $2,320/5x100 Tests
Western Blot (WB)
( All lanes : Anti-LTF Antibody at 1:4000 dilution Lane 1: A549 whole cell lysate Lane 2: Human plasma tissue lysate Lane 3: Human fetal kidney tissue lysate Lane 4: Human breast tissue lysate Lane 5: Human uterus tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
LTF, Polyclonal Antibody (Cat# AAA9204289)
Full Name
LTF Antibody
Gene Names
LTF; LF; HLF2; GIG12; HEL110
Reactivity
Human, mouse
Applications
WB, EIA, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Midkine, Active Protein (Cat# AAA691539)
Full Name
Human Midkine
Gene Names
MDK; MK; ARAP; NEGF2
Reactivity
Human
Purity
> 98 % (SDS-PAGE, HPLC)
Pricing
$395/0.02 mg | $250/0.005 mg | $1,565/5x0.02 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-CD62L (L-selectin) antibody MEL-14 Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of MEL-14 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
CD62L, Monoclonal Recombinant Antibody (Cat# AAA488435)
Full Name
Anti-CD62L [MEL-14]
Gene Names
Sell; Lnhr; CD62L; Ly-22; Lyam1; Ly-m22; Lyam-1; LECAM-1; AI528707; L-selectin
Reactivity
Mouse
Applications
BL, IP, FC/FACS, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Rabbit Polyclonal Antibody.)
CD15/FUT4, Polyclonal Antibody (Cat# AAA4380927)
Full Name
CD15/FUT4 (Reed-Sternberg Cell Marker)
Gene Names
FUT4; LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data #1
(Human peripheral blood monocytes stained with Mouse anti Human Siglec-5/Siglec-14 (MBS224725) followed by rabbit F(ab')2 anti mouse IgG:FITC (MBS235160))
SIGLEC-5/SIGLEC-14, Monoclonal Antibody (Cat# AAA224725)
Full Name
MOUSE ANTI HUMAN SIGLEC-5/SIGLEC-14
Gene Names
SIGLEC5; CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
Applications
EIA, FC/FACS, IF, IP
Pricing
$340/0.1 mg | $1,375/5x0.1 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215790)
Full Name
RAT ANTI MOUSE DECTIN-1:Biotin
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$460/0.1 mg | $1,910/5x0.1 mg
GRO/KC, Active Protein (Cat# AAA650941)
Full Name
GRO/KC, Recombinant, Mouse (Cxcl1, Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, alpha), Cinc1, CINC-1, C-X-C Motif Chemokine 1, Cytokine-induced Neutrophil Chemoattractant 1, Gro1, Growth-regulated alpha Protein, Platelet-derive
Gene Names
Cxcl1; Gro1; CINC-1
Purity
Highly Purified
98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Pricing
$515/0.02 mg | $365/0.005 mg | $2,305/5x0.02 mg
Western Blot (WB)
(Western blot analysis of Hela cell lysate, using LCP1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
LCP1, Polyclonal Antibody (Cat# AAA9608038)
Full Name
LCP1 Antibody
Gene Names
LCP1; LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
NAP2, Active Protein (Cat# AAA650335)
Full Name
NAP2 (Neutrophil Activating Protein 2, CXCL7, C-X-C motif chemokine 7, PPBP, pro-platelet basic protein)
Gene Names
NAP1L4; NAP2; NAP2L; hNAP2; NAP1L4b
Purity
Highly Purified
~98% (SDS-PAGE)
~98% (SDS-PAGE)
Pricing
$375/0.002 mg | $1,680/5x0.002 mg
CXCL5, Active Protein (Cat# AAA637775)
Full Name
CXCL5, Recombinant, Mouse (C-X-C Motif Chemokine 5, Cytokine LIX, Small-inducible Cytokine B5, Scyb5)
Gene Names
CXCL5; SCYB5; ENA-78
Purity
Highly Purified
~97% (HPLC, SDS-PAGE)
~97% (HPLC, SDS-PAGE)
Pricing
$485/0.02 mg | $2,170/5x0.02 mg
Western Blot (WB)
((0. 05ug/ml) staining of Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
LTF, Polyclonal Antibody (Cat# AAA422219)
Full Name
Goat anti-LTF Antibody
Gene Names
LTF; LF; HLF2; GIG12; HEL110
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
EIA, WB, IHC, EIA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215796)
Full Name
RAT ANTI MOUSE DECTIN-1:FITC
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$210/0.025 mg | $800/5x0.025 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215794)
Full Name
RAT ANTI MOUSE DECTIN-1:FITC
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$280/0.05 mg | $1,115/5x0.05 mg
Interleukin 8, 77aa, Active Protein (Cat# AAA650699)
Full Name
Interleukin 8, Recombinant, Human, 77aa (IL-8)
Gene Names
IL8; NAF; GCP1; LECT; LUCT; NAP1; CXCL8; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
Purity
Highly Purified
98% by SDS-PAGE and HPLC analysis. Endotoxin: 0.1ng/ug
98% by SDS-PAGE and HPLC analysis. Endotoxin: 0.1ng/ug
Pricing
$480/0.025 mg | $330/0.005 mg | $2,150/5x0.025 mg
Interleukin 8, 72aa, Active Protein (Cat# AAA650368)
Full Name
Interleukin 8, Recombinant, Human, 72aa (IL-8)
Gene Names
IL8; NAF; GCP1; LECT; LUCT; NAP1; CXCL8; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
Purity
Highly Purified
98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug)
98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug)
Pricing
$480/0.025 mg | $330/0.005 mg | $2,150/5x0.025 mg
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216590)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN
Applications
FC/FACS, IF, WB
Pricing
$525/0.25 mg | $2,210/5x0.25 mg
Flow Cytometry (FC/FACS)
(Human peripheral blood neutrophils are stained with Anti-Human CD13 Monoclonal Antibody(APC Conjugated)(filled gray histogram). Unstained neutrophils (empty black histogram) are used as control.)
CD13, Monoclonal Antibody (Cat# AAA2556506)
Full Name
Anti-Human CD13 Monoclonal Antibody (APC Conjugated)
Gene Names
ANPEP; APN; CD13; LAP1; P150; PEPN; GP150
Reactivity
Human
Applications
FC/FACS
Pricing
$215/100 Tests | $280/200 Tests | $165/50 Tests | $130/20 Tests | $1,255/5x200 Tests
