Western Blot (WB)
((0. 05ug/ml) staining of Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat LTF Polyclonal Antibody | anti-LTF antibody
Goat anti-LTF Antibody
Immunohistochemistry: In paraffin embedded blood vessel shows strong staining of cytoplasm of neutrophils. Recommended concentration, 2-4ug/ml.
Western Blot: Approx.85kDa band observed in Human Peripheral Mononucleocyte lysates (calculated MW of 78.2kDa according to NP_002334.2). Recommended concentration: 0. 05-0.2ug/ml.
Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant LTF captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml.
Western Blot (WB)
((0. 05ug/ml) staining of Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 05ug/ml) staining of Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Blood Vessel. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Blood Vessel. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Testing Data
((5ug/ml) as the reporter with as the capture rabbit antibody (5ug/ml).)
Testing Data
((5ug/ml) as the reporter with as the capture rabbit antibody (5ug/ml).)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
Uniprot Description
LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter.
Protein type: Protease; EC 3.4.21.-; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 3p21.31
Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; nucleus; protein complex; secretory granule; specific granule
Molecular Function: DNA binding; heparin binding; iron ion binding; protein binding; protein serine/threonine kinase activator activity; serine-type endopeptidase activity
Biological Process: activation of NF-kappaB transcription factor; antibacterial humoral response; antifungal humoral response; cell redox homeostasis; cellular protein metabolic process; humoral immune response; innate immune response in mucosa; ion transport; iron assimilation by chelation and transport; negative regulation of apoptosis; negative regulation of ATPase activity; negative regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of viral genome replication; negative regulation of viral reproduction; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of osteoblast differentiation; positive regulation of osteoblast proliferation; positive regulation of toll-like receptor 4 signaling pathway; proteolysis; regulation of cytokine production; regulation of tumor necrosis factor production; retinal homeostasis; transcription, DNA-dependent
Research Articles on LTF
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Product Notes
The LTF ltf (Catalog #AAA422219) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-LTF Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's LTF can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded blood vessel shows strong staining of cytoplasm of neutrophils. Recommended concentration, 2-4ug/ml. Western Blot: Approx.85kDa band observed in Human Peripheral Mononucleocyte lysates (calculated MW of 78.2kDa according to NP_002334.2). Recommended concentration: 0. 05-0.2ug/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant LTF captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml. Researchers should empirically determine the suitability of the LTF ltf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ENYKSQQSSD PDP. It is sometimes possible for the material contained within the vial of "LTF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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