GRO gamma Active Protein

GRO gamma, Recombinant, Human

Cat. Num. AAA650677

• Purity: Molecular Biology Grade; 98% by SDS-PAGE and HPLC analyses.
• Synonyms: GRO gamma; Recombinant; Human; GRO gamma active protein

• Host: Human
• Purity/Purification: Molecular Biology Grade; 98% by SDS-PAGE and HPLC analyses.
• Form/Format: Lyophilized protein without additives.

• Biological Activity: Maximal chemotactic activity on rat neutrophils is assayed in a modified Boyden chamber at 50ng/ml.
• Endotoxin Level: 0.1ng/ug.
• Reconstitution: Soluble in water and most aqueous buffers. The lyophilized protein should be reconstituted in water to a concentration of 100ng/ul. This solution can be diluted into water or other buffered solutions or stored at -20 degree C for future use.
• Preparation and Storage: -20 degree C; Stability: The lyophilized protein, though stable at room temperature, is best stored desiccated below 0 degree C. Reconstituted rat GRO- should be stored in working aliquots at -20 degree C.

Related Product Information for GRO gamma active protein

CXCL3/GROg is a small cytokine belonging to the CXC chemokine family. CXCL3 controls migration and adhesion of monocytes and mediates it effects on its target cell by interacting with a cell surface chemokine receptor called CXCR2. The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively.Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

Product Categories/Family for GRO gamma active protein

Growth Factors; Cytokines; Growth Factors-GRO

NCBI and Uniprot Product Information

• NCBI GI #: 30023531
• Molecular Weight: 7.9kD
• NCBI Official Full Name: GRO-gamma

NCBI Description

This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL3: Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; neutrophil chemotaxis; immune response; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; inflammatory response; regulation of cell proliferation