Interleukin 8, 72aa Active Protein | IL8 active protein

Interleukin 8, Recombinant, Human, 72aa (IL-8)

Cat. Num. AAA650368

• Gene Names: IL8; NAF; GCP1; LECT; LUCT; NAP1; CXCL8; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
• Purity: Highly Purified; 98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug)
• Synonyms: Interleukin 8; 72aa; Recombinant; Human; 72aa (IL-8); IL8 active protein

• Host: Recombinant Human (E Coli)
• Purity/Purification: Highly Purified; 98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug)
• Form/Format: Supplied as a lyophilized powder.. Reconstitute in sterile ddH2O, 0.1% HSA or BSA to a concentration 0.1 mg/ml.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Lys-Glu-Leu.

• Biological Activity: rHuIL-8 is fully biologically active when compared to standard. Specific Activity of IL-8 in chemotaxis of donor PBL neutrophils, threshold concentration corresponding to 10-100ng/ml.
• Dimers and Aggregates: 1% as determined by silver-stained SDS-PAGE gel analysis.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).; 2. Analysis by RP-HPLC, using a standard solution of Eotaxin as a Reference Standard.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, 0.1% HSA or BSA, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for IL8 active protein

Recombinant Human IL-8 is a single, non-glycosylated polypeptide chain containing 72 amino acids (7-77) and having a molecular mass of 8452D. Recombinant Interleukin-8 is purified by proprietary chromatographic techniques.

Product Categories/Family for IL8 active protein

Growth Factors; Cytokines; Growth Factors-Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 10834978
• NCBI GeneID: 3576
• NCBI Accession #: NP_000575.1
• NCBI GenBank Nucleotide #: NM_000584.3
• UniProt Accession #: P10145; Q6FGF6; Q6LAE6; Q96RG6; Q9C077; Q9UCE1; Q9UCR8; Q9UCR9; Q9UCS0; B2R4L8
• Molecular Weight: 11,098 Da
• NCBI Official Full Name: interleukin-8
• NCBI Official Synonym Full Names: interleukin 8
• NCBI Official Symbol: IL8
• NCBI Official Synonym Symbols: NAF; GCP1; LECT; LUCT; NAP1; CXCL8; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
• NCBI Protein Information: interleukin-8; emoctakin; OTTHUMP00000199824; OTTHUMP00000199825; C-X-C motif chemokine 8; T cell chemotactic factor; T-cell chemotactic factor; neutrophil-activating peptide 1; chemokine (C-X-C motif) ligand 8; beta-thromboglobulin-like protein; granulocyte chemotactic protein 1; tumor necrosis factor-induced gene 1; alveolar macrophage chemotactic factor I; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; small inducible cytokine subfamily B, member 8; lymphocyte-derived neutrophil-activating factor; lymphocyte derived neutrophil activating peptide; lung giant cell carcinoma-derived chemotactic protein; beta endothelial cell-derived neutrophil activating peptide
• UniProt Protein Name: Interleukin-8
• UniProt Gene Name: IL8
• UniProt Synonym Gene Names: CXCL8
• UniProt Entry Name: IL8_HUMAN

NCBI Description

The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq]

Uniprot Description

Function: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. Ref.15 Ref.24 Ref.27

Subunit structure: Homodimer.

Subcellular location: Secreted.

Post-translational modification: Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.

Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.