Western Blot
(Western Blot analysis using Proteinase 3 antibodyWestern Blot showing PRTN3 antibody used against PRTN3 (aa28-256)-hIgGFc transfected HEK293 cell lysate (1).)
Mouse Proteinase 3 Monoclonal Antibody | anti-PRTN3 antibody
Proteinase 3 antibody
Gene Names
PRTN3; MBN; MBT; NP4; P29; PR3; ACPA; AGP7; NP-4; PR-3; CANCA; C-ANCA
Applications
ELISA, Western Blot
Purity
Proteinase 3 antibody was purified by protein G affinity chromatography.
Synonyms
Proteinase 3; Monoclonal Antibody; Proteinase 3 antibody; Monoclonal Proteinase 3; Anti-Proteinase 3; Proteinase-3; PR-3; PR3; anti-PRTN3 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3B4
Purity/Purification
Proteinase 3 antibody was purified by protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS containing 50% glycerol and 0.03% sodium azide.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
254
Applicable Applications for anti-PRTN3 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: 1:10,000
WB: 1:500-1:2000
WB: 1:500-1:2000
Biological Significance
Proteinase 3 also known as PRTN3 is an enzyme which in humans is encoded by the PRTN3 gene. PRTN3 is a serine protease enzyme expressed mainly in neutrophil granulocytes. Its exact role in the function of the neutrophil is unknown but in human neutrophils proteinase 3 contributes to the proteolytic generation of antimicrobial peptides.
Cross-Reactivity
Human
Immunogen
Proteinase 3 antibody was raised in Mouse using a purified recombinant fragment of human PRTN3 expressed in E Coli as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot
(Western Blot analysis using Proteinase 3 antibodyWestern Blot showing PRTN3 antibody used against PRTN3 (aa28-256)-hIgGFc transfected HEK293 cell lysate (1).)
Western Blot
(Western Blot analysis using Proteinase 3 antibodyWestern Blot showing PRTN3 antibody used against PRTN3 (aa28-256)-hIgGFc transfected HEK293 cell lysate (1).)
Related Product Information for anti-PRTN3 antibody
Mouse monoclonal Proteinase 3 antibody
Product Categories/Family for anti-PRTN3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
27,807 Da
NCBI Official Full Name
proteinase 3
NCBI Official Synonym Full Names
proteinase 3
NCBI Official Symbol
PRTN3
NCBI Official Synonym Symbols
MBN; MBT; NP4; P29; PR3; ACPA; AGP7; NP-4; PR-3; CANCA; C-ANCA
NCBI Protein Information
myeloblastin
UniProt Protein Name
Myeloblastin
Protein Family
UniProt Gene Name
PRTN3
UniProt Synonym Gene Names
MBN; PR-3; PR3; NP-4
UniProt Entry Name
PRTN3_HUMAN
Uniprot Description
PRTN3: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Belongs to the peptidase S1 family. Elastase subfamily.
Protein type: Cell cycle regulation; EC 3.4.21.76; Protease
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: extracellular matrix; extracellular space; plasma membrane; cytosol
Molecular Function: enzyme binding; serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: negative regulation of phagocytosis; collagen catabolic process; positive regulation of cell proliferation; blood coagulation; proteolysis
Research Articles on PRTN3
Similar Products
Product Notes
The PRTN3 prtn3 (Catalog #AAA831940) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Proteinase 3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 1:10,000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PRTN3 prtn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Proteinase 3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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