Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Rabbit Polyclonal Antibody.)
Rabbit CD15/FUT4 Polyclonal Antibody | anti-FUT4 antibody
CD15/FUT4 (Reed-Sternberg Cell Marker)
Gene Names
FUT4; LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
CD15/FUT4; Polyclonal Antibody; CD15/FUT4 (Reed-Sternberg Cell Marker); 3 Fucosyl N Acetyl Lactosamine; Alpha (1;3) Fucosyltransferase; Alpha 13 fucosyltransferase FucT; ELAM Ligand Fucosyltransferase; ELFT; FCT3A; Fuc-TIV; Fucosyltransferase 4 Alpha 1 3 Fucosyltransferase Myeloid Specific; Fucosyltransferase 4; Galactoside 3 L Fucosyltransferase; Lewis X; LeX; SSEA1; Stage Specific Embryonic Antigen 1; anti-FUT4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present ons disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & Azide at 1.0mg/ml.
Sequence Length
530
Applicable Applications for anti-FUT4 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
Application Notes
FC/FACS: 2-4ug/million cells
IF: 2-4ug/ml
Immunohistochemistry (Formalin-fixed) (5-10ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
IF: 2-4ug/ml
Immunohistochemistry (Formalin-fixed) (5-10ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Positive Control
U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma.
Immunogen
Purified human neutrophils
Cellular Localization
Cell Surface and granular paranuclear
Hu-chromosome Location
11q21
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Rabbit Polyclonal Antibody.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Rabbit Polyclonal Antibody.)
References
Hakomori, S. 1992. LeX and related structures as adhesion molecules. Histochem. J. 24: 771-776
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~220kDa
NCBI Official Full Name
alpha-(1,3)-fucosyltransferase 4
NCBI Official Synonym Full Names
fucosyltransferase 4
NCBI Official Symbol
FUT4
NCBI Official Synonym Symbols
LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV
NCBI Protein Information
alpha-(1,3)-fucosyltransferase 4
UniProt Protein Name
Alpha-(1,3)-fucosyltransferase 4
Protein Family
UniProt Gene Name
FUT4
UniProt Synonym Gene Names
ELFT; FCT3A; Fuc-TIV; FucT-IV
NCBI Description
The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009]
Uniprot Description
May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
Research Articles on FUT4
Similar Products
Product Notes
The FUT4 fut4 (Catalog #AAA4380927) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD15/FUT4 (Reed-Sternberg Cell Marker) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD15/FUT4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin. FC/FACS: 2-4ug/million cells IF: 2-4ug/ml Immunohistochemistry (Formalin-fixed) (5-10ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the FUT4 fut4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD15/FUT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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