Midkine Active Protein | Mdk active protein

Recombinant Mouse Midkine

Cat. Num. AAA145112

• Gene Names: Mdk; MK; Mek
• Purity: Greater than 95.0% as determined by HPLC and SDS-PAGE.
• Synonyms: Midkine; Recombinant Mouse Midkine; Midkine Mouse; Midkine Mouse Recombinant; NEGF-2; Neurite Growth-Promoting Factor 2; MK; Neurite outgrowth-promoting protein; Midgestation and kidney protein; Amphiregulin-associated protein; ARAP; Neurite outgrowth-promoting factor 2; FLJ27379; MK1; NEGF2; mMidkine; Mdk active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by HPLC and SDS-PAGE.
• Form/Format: Lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: VAKKKEKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRVHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK TKAKKGKGKD
• Sequence Length: 140

• Solubility: It is recommended to reconstitute the lyophilized Midkine Mouse in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Fully biologically active when compared to standard. Determined by its ability to chemoattract human neutrophils using a concentration range of 10-100 ng/ml corresponding to a specific activity of 10,000-100,000IU/mg.
• Preparation and Storage: Lyophilized Midkine Mouse although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Midkine Mouse should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.

Related Product Information for Mdk active protein

Description: Midkine Mouse Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 120 amino acids and having a molecular mass of 13.3kDa.The Midkine Mouse is purified by proprietary chromatographic techniques.

Introduction: Midkine (MK) is the product of a retinoic acid responsive gene, MK, and is a member of a family of heparin binding factors. It contains 121 amino acid residues including 10 conserved cysteine residues, all of which appear to be disulphide linked.Midkine is expressed during embryogenesis, showing an expression pattern that suggests functions in neurogenesis, cell migration, secondary organogenetic induction, and mesoderm-epithelial interaction.The widespread downregulation of MK in the adult human is reverted in a number of cancers, in which polypeptides are able to act as both transforming growth factors and promoters of angiogenesis.Midkine (MK), induces chemotaxis of human neutrophils and was found to trigger mobilization of intracellular calcium of these cells.Midkine induces histamine release from rat peritoneal mast cells with a rapid response in a dose dependent manner.Midkine is also a potent stimulator of collagen and glycosaminoglycan synthesis.

Product Categories/Family for Mdk active protein

Cytokines and Growth Factors; Neurotrophins; Midkine

NCBI and Uniprot Product Information

• NCBI GI #: 59894790
• NCBI GeneID: 17242
• NCBI Accession #: NP_001012335.1
• NCBI GenBank Nucleotide #: NM_001012335.2
• UniProt Accession #: P12025
• Molecular Weight: 15,434 Da
• NCBI Official Full Name: midkine isoform a
• NCBI Official Synonym Full Names: midkine
• NCBI Official Symbol: Mdk
• NCBI Official Synonym Symbols: MK; Mek
• NCBI Protein Information: midkine; retinoic acid-induced differentiation factor; retinoic acid-responsive protein
• UniProt Protein Name: Midkine
• Protein Family: Midkine
• UniProt Gene Name: Mdk
• UniProt Synonym Gene Names: Mk; MK
• UniProt Entry Name: MK_MOUSE

NCBI Description

This gene encodes a secreted growth factor that belongs to the pleiotrophin/midkine heparin-binding protein family and functions in a variety of biological processes. The encoded cytokine promotes the growth, differentiation, survival and migration of several target cells including leucocytes involved in inflammation. This protein plays a role in the formation of scar tissue and intraperitoneal adhesions, and promotes neurite outgrowth and neuron survival. The protein encoded by this gene is associated with obesity and inhibition of insulin signaling in fat cells. A pseudogene of this gene is present on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

MDK: Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development. Belongs to the pleiotrophin family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: cell projection; cytoplasm; extracellular region

Molecular Function: heparin binding; growth factor activity

Biological Process: cell migration; behavioral fear response; cerebellar granular layer development; adrenal gland development; short-term memory; multicellular organismal development; positive regulation of transcription, DNA-dependent; hippocampus development; positive regulation of cell division; response to wounding; negative regulation of neuron apoptosis; cell differentiation; defecation; regulation of behavior