Rabbit HCK Polyclonal Antibody | anti-HCK antibody

Phospho-HCK (Tyr522) Antibody

Cat. Num. AAA9610573

• Gene Names: HCK; JTK9; p59Hck; p61Hck
• Reactivity: Human; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
• Applications: Western Blot, ELISA
• Purity: Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Synonyms: HCK; Polyclonal Antibody; Phospho-HCK (Tyr522) Antibody; Bmk; Hck 1; Hck; HCK_HUMAN; Hemopoietic cell kinase; JTK9; p59-HCK/p60-HCK; p59Hck; p59HCK/p60HCK; p61Hck; Tyrosine protein kinase HCK; Tyrosine-protein kinase HCK; anti-HCK antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-HCK (Tyr522) Antibody detects endogenous levels of HCK only when phosphorylated at Tyr522.
• Purity/Purification: Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 505

• Applicable Applications for anti-HCK antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human HCK around the phosphorylation site of Tyr522.
• Tissue Specificity: Detected in monocytes and neutrophils (at protein level). Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes. Detected in tonsil.
• Subcellular Location: Lysosome; Cytosol; Plasma Membrane; Lysosome; Nucleus; Cytoskeleton; Golgi Apparatus; Plasma Membrane; Cytosol
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of Phospho-HCK (Tyr522) in lysates of Jurkat, using Phospho-HCK (Tyr522) Antibody.)

Related Product Information for anti-HCK antibody

Description: Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.

Post Translational Modifications: Phosphorylated on several tyrosine residues. Autophosphorylated. Becomes rapidly phosphorylated upon activation of the immunoglobulin receptors FCGR1A and FCGR2A. Phosphorylation by the BCR-ABL fusion protein mediates activation of HCK. Phosphorylation at Tyr-411 increases kinase activity. Phosphorylation at Tyr-522 inhibits kinase activity. Kinase activity is not required for phosphorylation at Tyr-522, suggesting that this site is a target of other kinases. Ubiquitinated by CBL, leading to its degradation via the proteasome. Isoform 2 palmitoylation at position 2 requires prior myristoylation. Palmitoylation at position 3 is required for caveolar localization of isoform 2.

Subunit Structure: Interacts (via SH2 domain) with FLT3 (tyrosine phosphorylated). Interacts with VAV1, WAS and RAPGEF1 (By similarity). Interacts (via SH3 domain) with HIV-1 Nef and Vif. This interaction stimulates its tyrosine-kinase activity. Interacts (via SH3 domain) with HEV ORF3 protein. Interacts with ARRB1 and ARRB2. Interacts with ADAM15. Interacts with FASLG. Interacts with CBL. Interacts with FCGR1A; the interaction may be indirect. Interacts with IL6ST. Interacts (via SH3 domain) with ELMO1. Interacts (via SH3 domain) with TP73. Interacts with YAP1. Interacts with ABL1 and ITGB1, and thereby recruits ABL1 to activated ITGB1. Interacts (via SH3 domain) with WDCP.

Similarity: The SH3 domain mediates binding to HIV-1 Nef. Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 287326139
• NCBI GeneID: 3055
• NCBI Accession #: NP_001165600.1
• NCBI GenBank Nucleotide #: NP_001165600.1
• UniProt Accession #: P08631
• Molecular Weight: Observed: 59 kDa; Predicted: 60 kDa
• NCBI Official Full Name: tyrosine-protein kinase HCK isoform b
• NCBI Official Synonym Full Names: HCK proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: HCK
• NCBI Official Synonym Symbols: JTK9; p59Hck; p61Hck
• NCBI Protein Information: tyrosine-protein kinase HCK
• UniProt Protein Name: Tyrosine-protein kinase HCK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: HCK
• UniProt Entry Name: HCK_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010]

Uniprot Description

Hck: a protein-tyrosine kinase of the Src family that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms that have different subcellular localization.

Protein type: Oncoprotein; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; EC 2.7.10.2; Kinase, protein; TK group; Src family

Chromosomal Location of Human Ortholog: 20q11-q12

Cellular Component: Golgi apparatus; extrinsic to internal side of plasma membrane; transport vesicle; focal adhesion; cell projection; lysosome; actin filament; caveola; nucleus; cytosol

Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: peptidyl-tyrosine phosphorylation; respiratory burst after phagocytosis; viral reproduction; protein amino acid autophosphorylation; regulation of phagocytosis; protein amino acid phosphorylation; regulation of cell shape; leukocyte migration during immune response; regulation of transcription factor activity; leukocyte degranulation; positive regulation of cell proliferation; lipopolysaccharide-mediated signaling pathway; mesoderm development; inflammatory response; innate immune response-activating signal transduction; cell adhesion; cell differentiation; integrin-mediated signaling pathway; cytokine and chemokine mediated signaling pathway; regulation of defense response to virus by virus; positive regulation of actin filament polymerization; regulation of inflammatory response; innate immune response; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis