Rat CD18 Monoclonal Antibody | anti-CD18 antibody

RAT ANTI HUMAN CD18:FITC

Cat. Num. AAA211045

• Gene Names: ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD18; Monoclonal Antibody; RAT ANTI HUMAN CD18:FITC; anti-CD18 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: YFC118.3
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1mg/ml (varies by lot)
• Sequence Length: 769

• Applicable Applications for anti-CD18 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Maximum Dilution: Neat
• Perservative Stabilisers: 0.09% Sodium Azide; 1% Bovine Serum Albumin; Preparation
• Immunogen: Human neutrophils; Fusion Partners
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)

Testing Data

(Published customer image: Rat anti Human CD18 antibody,clone YFC118.3 used for flow cytometryImage caption: LFA-1 activation analyses by Flow Cytometry. (A) Profiles of CD11a, CD18 activation epitope (CD18act, representing œhigh affinity conformation) and CD18 (CD18tot) expression by Teff and Treg#1 cells pre-incubated or not with indicated antibodies. Anti-CD28 and anti-CTLA-4 were used at 10 ug/ml. (B) Histograms of Mean Fluorescent Intensity (MFI) of CD11a, CD18act and CD18tot expressed on Teff and Treg#1 cells. Ratio CD18act MFI: CD18tot was established to analyze LFA-1 high affinity conformation in indicated conditions. Data are representative of more than three different experiments. Filled gray, negative control; Red line, Teff and Treg cells alone; Green line, cells with APC; Blue line, cells with APC and anti-CD28; and Black line: cells with APC, anti-CD28 and anti-CTLA-4.From: Dilek N, Poirier N, Hulin P, Coulon F, Mary C, et al. (2013) Targeting CD28, CTLA-4 and PD-L1 Costimulation Differentially Controls Immune Synapses and Function of Human Regulatory and Conventional T-Cells. PLoS ONE 8(12): e83139.)

Testing Data

(Staining of human peripheral blood granulocytes with Rat anti Human CD18:RPE (MBS213845))

Testing Data

(Staining of human peripheral blood lymphocytes with Rat anti Human CD18:Biotin (MBS210497))

Testing Data

(Staining of human peripheral blood lymphocytes with Rat anti Human CD18 (MBS213057))

Testing Data

(Staining of human peripheral blood granulocytes with Rat anti Human CD18:FITC)

Testing Data

(Published customer image: Mouse anti Human CD18 antibody, clone YFC118.3 used for immunofluorescence studies using formalin fixed paraffin embedded material following antigen retrieval with citrate buffer at pH6.0Image caption:Subretinal MPs accumulate on the retinal pigment epithelium in AMDA -D Confocal microscopy of IBA-1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z-stack projection; (C): oblique Z-stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E -G Double-labeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA-1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z-stack of a subretinal IBA-1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.Data information: Controls omitting the primary antibody showed no staining apart from the autofluorescence. AZ: atrophic zone; LD: large drusen. Scale bar: 50 um (A -D); 10 um (E -H).From: Levy O, Calippe B, Lavalette S, Hu SJ, Raoul W, Dominguez E, Housset M, Paques M, Sahel JA, Bemelmans AP, Combadiere C, Guillonneau X, Sennlaub F. Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration. EMBO Mol Med. 2015 Jan 20. pii: e201404524.)

Testing Data

(Staining of human peripheral blood granulocytes with CD18: Alexa Fluor 647 (MBS213057A647))

Related Product Information for anti-CD18 antibody

Rat anti Human CD18 antibody, clone YFC118.3 was clustered at the Fourth International Workshop on Leucocyte Differentiation Antigen (code number N221) as recognising the CD18 antigen. The CD18 antigen is an integral membrane glycoprotein of 95kD molecular weight - known as the beta chain, of the LFA-1 complex. The CD18 antigen is non-covalently linked to CD11a, b, c molecules. The main cellular reactivity of Rat anti Human CD18 antibody, clone YFC118.3 is a strong reactivity with Leucocytes (platelets negative). The CD18 molecule is the receptor for ICAM-1 and is thus important for cell adhesion.

NCBI and Uniprot Product Information

• NCBI GI #: 89191865
• NCBI GeneID: 3689
• NCBI Accession #: NP_000202.2
• NCBI GenBank Nucleotide #: NM_000211.3
• UniProt Accession #: P05107; Q16418; Q53HS5; Q9UD72; B3KTS8; D3DSM1
• Molecular Weight: 84,782 Da
• NCBI Official Full Name: integrin beta-2
• NCBI Official Synonym Full Names: integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
• NCBI Official Symbol: ITGB2
• NCBI Official Synonym Symbols: LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
• NCBI Protein Information: integrin beta-2; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor); complement receptor C3 beta-subunit; integrin beta chain, beta 2; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
• UniProt Protein Name: Integrin beta-2
• Protein Family: CD180 antigen
• UniProt Gene Name: ITGB2
• UniProt Synonym Gene Names: CD18; MFI7
• UniProt Entry Name: ITB2_HUMAN

NCBI Description

This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

ITGB2: the integrin beta 2 subunit. Can combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Participates in cell adhesion as well as cell-surface mediated signaling. Defects are the cause of leukocyte adhesion deficiency type I (LAD1).

Protein type: Cell adhesion; Receptor, misc.; Membrane protein, integral; Cell surface; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cell surface; membrane; plasma membrane; integrin complex; receptor complex; vesicle; lipid raft

Molecular Function: protein binding; protein heterodimerization activity; metal ion binding; protein complex binding; cell adhesion molecule binding; ICAM-3 receptor activity; glycoprotein binding; protein kinase binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; apoptosis; cell-matrix adhesion; natural killer cell activation; receptor clustering; activation of NF-kappaB transcription factor; regulation of cell shape; leukocyte adhesion; cell-cell signaling; cellular extravasation; inflammatory response; cell adhesion; toll-like receptor 4 signaling pathway; regulation of peptidyl-tyrosine phosphorylation; aging; integrin-mediated signaling pathway; neutrophil chemotaxis; regulation of immune response; activated T cell proliferation; leukocyte migration during inflammatory response; heterotypic cell-cell adhesion; positive regulation of angiogenesis; toll-like receptor signaling pathway; innate immune response; endothelial cell migration; blood coagulation; leukocyte migration

Disease: Leukocyte Adhesion Deficiency, Type I