Testing Data
(Texas red conjugated Goat anti Mouse IgG antibody used for the detection of bound anti CD80 antibody by immunofluorescenceImage caption:Immunolabelling of Mphi by the monocyte/Mphi marker CD14 and the M1 Mphi marker CD80. Mphi were seeded at a density of 5.7×104 cells/cm2, with (coculture) or without the same number of MDA-MB-231 cells. In each case, the culture medium was replaced daily. 5 days later, all cultures were treated with 1 mug/ml LPS and 5 ng/ml IFNgamma for 24 h before washing and labelling. CD14 fluorescent labelling is green, CD80 is red. Panel A: without TM, it can be seen that CD80 was expressed on activated Mphi cultured alone and was not detectable on Mphi in the cocultures. Panel B: in presence of TM, no CD80-positive cells were found in any of the cultures.From: Joimel U, Gest C, Soria J, Pritchard LL, Alexandre J, Laurent M, Blot E, Cazin L, Vannier JP, Varin R, Li H, Soria C.Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate.BMC Cancer. 2010 Jul 17;10:375)
Goat anti-Mouse IgG Polyclonal Antibody | anti-IgG antibody
Goat Anti Mouse IgG: FITC
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Texas red conjugated Goat anti Mouse IgG antibody used for the detection of bound anti CD80 antibody by immunofluorescenceImage caption:Immunolabelling of Mphi by the monocyte/Mphi marker CD14 and the M1 Mphi marker CD80. Mphi were seeded at a density of 5.7×104 cells/cm2, with (coculture) or without the same number of MDA-MB-231 cells. In each case, the culture medium was replaced daily. 5 days later, all cultures were treated with 1 mug/ml LPS and 5 ng/ml IFNgamma for 24 h before washing and labelling. CD14 fluorescent labelling is green, CD80 is red. Panel A: without TM, it can be seen that CD80 was expressed on activated Mphi cultured alone and was not detectable on Mphi in the cocultures. Panel B: in presence of TM, no CD80-positive cells were found in any of the cultures.From: Joimel U, Gest C, Soria J, Pritchard LL, Alexandre J, Laurent M, Blot E, Cazin L, Vannier JP, Varin R, Li H, Soria C.Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate.BMC Cancer. 2010 Jul 17;10:375)
Testing Data
(Texas red conjugated Goat anti Mouse IgG antibody used for the detection of bound anti CD80 antibody by immunofluorescenceImage caption:Immunolabelling of Mphi by the monocyte/Mphi marker CD14 and the M1 Mphi marker CD80. Mphi were seeded at a density of 5.7×104 cells/cm2, with (coculture) or without the same number of MDA-MB-231 cells. In each case, the culture medium was replaced daily. 5 days later, all cultures were treated with 1 mug/ml LPS and 5 ng/ml IFNgamma for 24 h before washing and labelling. CD14 fluorescent labelling is green, CD80 is red. Panel A: without TM, it can be seen that CD80 was expressed on activated Mphi cultured alone and was not detectable on Mphi in the cocultures. Panel B: in presence of TM, no CD80-positive cells were found in any of the cultures.From: Joimel U, Gest C, Soria J, Pritchard LL, Alexandre J, Laurent M, Blot E, Cazin L, Vannier JP, Varin R, Li H, Soria C.Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate.BMC Cancer. 2010 Jul 17;10:375)
Testing Data
(Biotin conjugated Goat anti Mouse IgG (103008) used in conjunction with horseradish peroxidase conjugated streptavidin (710005) for the detection of anti tumor derived antigen IgG by ELISA.Image caption:Local melanoma tumors generate systemic circulating IgG levels. (A). Outline of local tumor injection experiment. B16.F10-tdTom+ cells were s.c. injected into C57BL/6 mice on day 0 and allowed to proliferate for 30 days. In some mice, B16.F10-tdTom lysate was additionally s.c. injected to test whether IgG production can be boosted. (B) Brightfield (top) and red fluorescent (bottom) stereomicroscopic images of tumor-injected and contralateral footpads on day 30. (C) IgG titers in control and tumor-bearing mice on day 30, with and without additional B16.F10-tdTom+ cell lysate injection for 15 days. Each dot represents the serum of one mouse. Data in (C) are pooled from two independent experiments and were analyzed by a Kruskal-Wallis test with Dunn's multiple comparison test. **p )
Testing Data
(Biotin conjugated Goat anti Mouse IgG (103008) used in conjunction with horseradish peroxidase conjugated streptavidin (710005) for the detection of anti tumor derived antigen IgG by ELISA.Image caption:Local melanoma tumors generate systemic circulating IgG levels. (A). Outline of local tumor injection experiment. B16.F10-tdTom+ cells were s.c. injected into C57BL/6 mice on day 0 and allowed to proliferate for 30 days. In some mice, B16.F10-tdTom lysate was additionally s.c. injected to test whether IgG production can be boosted. (B) Brightfield (top) and red fluorescent (bottom) stereomicroscopic images of tumor-injected and contralateral footpads on day 30. (C) IgG titers in control and tumor-bearing mice on day 30, with and without additional B16.F10-tdTom+ cell lysate injection for 15 days. Each dot represents the serum of one mouse. Data in (C) are pooled from two independent experiments and were analyzed by a Kruskal-Wallis test with Dunn's multiple comparison test. **p )
Testing Data
(Texas Red conjugated Goat anti Mouse IgG antibody used for the detection of bound Mouse anti PSGL-1 antibody on neutrophil differentiated HL-60 cells by immunofluorescence.Image caption:Recognition of S. pneumoniae by PSGL-1 is a generic event. Co-localization of FAMS-SE-labeled S. pneumoniae clinical isolates of different serotypes with PSGL-1 in HL-60 cells differentiated to neutrophils. Cellular DNA was stained with Hoechst whereas PSGL-1 was visualized with mouse anti-human PSGL-1 antibody followed by a goat anti-mouse Texas-Red staining. (DIC, Differential interference contrast).From: Ramos-Sevillano E, Urzainqui A, de Andrés B, González-Tajuelo R, Domenech M, González-Camacho F, et al. (2016)PSGL-1 on Leukocytes is a Critical Component of the Host Immune Response against Invasive Pneumococcal Disease.PLoS Pathog 12(3): e1005500.)
Testing Data
(Texas Red conjugated Goat anti Mouse IgG antibody used for the detection of bound Mouse anti PSGL-1 antibody on neutrophil differentiated HL-60 cells by immunofluorescence.Image caption:Recognition of S. pneumoniae by PSGL-1 is a generic event. Co-localization of FAMS-SE-labeled S. pneumoniae clinical isolates of different serotypes with PSGL-1 in HL-60 cells differentiated to neutrophils. Cellular DNA was stained with Hoechst whereas PSGL-1 was visualized with mouse anti-human PSGL-1 antibody followed by a goat anti-mouse Texas-Red staining. (DIC, Differential interference contrast).From: Ramos-Sevillano E, Urzainqui A, de Andrés B, González-Tajuelo R, Domenech M, González-Camacho F, et al. (2016)PSGL-1 on Leukocytes is a Critical Component of the Host Immune Response against Invasive Pneumococcal Disease.PLoS Pathog 12(3): e1005500.)
Testing Data
(Texas Red conjugated Goat anti Mouse IgG antibody used for the detection of bound Mouse anti PSGL-1 antibody on neutrophil differentiated HL-60 cells by immunofluorescence.Image caption:Local co-localization of FAM-SE S. pneumoniae (green), Cellular DNA (blue) and PSGL-1 (red) is shown and quantified by plotting the fluorescence intensity and the distance (in mum).From: Ramos-Sevillano E, Urzainqui A, de Andrés B, González-Tajuelo R, Domenech M, González-Camacho F, et al. (2016)PSGL-1 on Leukocytes is a Critical Component of the Host Immune Response against Invasive Pneumococcal Disease.PLoS Pathog 12(3): e1005500.)
Testing Data
(Texas Red conjugated Goat anti Mouse IgG antibody used for the detection of bound Mouse anti PSGL-1 antibody on neutrophil differentiated HL-60 cells by immunofluorescence.Image caption:Local co-localization of FAM-SE S. pneumoniae (green), Cellular DNA (blue) and PSGL-1 (red) is shown and quantified by plotting the fluorescence intensity and the distance (in mum).From: Ramos-Sevillano E, Urzainqui A, de Andrés B, González-Tajuelo R, Domenech M, González-Camacho F, et al. (2016)PSGL-1 on Leukocytes is a Critical Component of the Host Immune Response against Invasive Pneumococcal Disease.PLoS Pathog 12(3): e1005500.)
Goat anti Mouse IgG antibody has been cross-adsorbed against mouse IgM, mouse IgA and human serum to reduce potential cross-reactivity.
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Product Notes
The IgG igh-3 (Catalog #AAA225563) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti Mouse IgG: FITC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IgG can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF). Researchers should empirically determine the suitability of the IgG igh-3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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