Mouse CD79a Antibody | anti-CD79a antibody

Anti-Hu CD79a FITC

Cat. Num. AAA1800749

• Gene Names: CD79A; IGA; MB-1
• Reactivity: Chicken, Rabbit, Opossum, Guinea pig, Horse, Cow, Pig, Mouse, Rat, Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD79a; Anti-Hu CD79a FITC; CD79a molecule; BCR alpha; Ig-alpha; MB-1; IGA; anti-CD79a antibody

• Host: Mouse
• Reactivity: Chicken, Rabbit, Opossum, Guinea pig, Horse, Cow, Pig, Mouse, Rat, Human
• Isotype: IgG1
• Clone Number: HM57
• Specificity: The antibody HM57 interacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.
• Purity/Purification: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
• Sequence Length: 226

• Applicable Applications for anti-CD79a antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: QC tested
• Immunogen: Synthetic peptide corresponding to amino acids 202-216 of human CD79a
• Conjugation: FITC
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Flow Cytometry (FC/FACS)

(Flow cytometry multicolor surface staining of human lymphocytes using anti-human CD19 (LT19) APC (10 ul reagent / 100 ul of peripheral whole blood) and intracellular staining of human lymphocytes using anti-human CD79a (HM57) FITC (4 ul reagent / 100 ul of peripheral whole blood) antibody.)

Related Product Information for anti-CD79a antibody

CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 4502685
• NCBI GeneID: 973
• NCBI Accession #: NP_001774.1
• NCBI GenBank Nucleotide #: NP_001774.1
• UniProt Accession #: P11912
• NCBI Official Full Name: B-cell antigen receptor complex-associated protein alpha chain isoform 1
• NCBI Official Synonym Full Names: CD79a molecule
• NCBI Official Symbol: CD79A
• NCBI Official Synonym Symbols: IGA; MB-1
• NCBI Protein Information: B-cell antigen receptor complex-associated protein alpha chain
• UniProt Protein Name: B-cell antigen receptor complex-associated protein alpha chain
• Protein Family: B-cell antigen receptor complex-associated protein
• UniProt Gene Name: CD79A
• UniProt Synonym Gene Names: IGA; MB1
• UniProt Entry Name: CD79A_HUMAN

NCBI Description

The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Ig-alpha: an integral membrane protein that associates with surface immunoglobulin B lymphocyte antigen receptor multimeric complex. Surface Ig non-covalently associates with 2 other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. Two alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: multivesicular body; integral to membrane; plasma membrane; B cell receptor complex; lipid raft; external side of plasma membrane

Molecular Function: transmembrane receptor activity

Biological Process: B cell proliferation; B cell receptor signaling pathway; B cell activation; B cell differentiation

Disease: Agammaglobulinemia 3, Autosomal Recessive