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1203 results for " neutrophils" - showing 100-150


Western Blot (WB) (Western blot analysis of HepG2 cell lysate, using PADI4 Antibody. The lane on the left is treated with the antigen-specific peptide.)

PADI4, Polyclonal Antibody (Cat# AAA9607341)

Full Name
PADI4 Antibody
Gene Names
PADI4; PAD; PAD4; PDI4; PDI5; PADI5
Reactivity
Human, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9601393 staining LOVO cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

P47, Polyclonal Antibody (Cat# AAA9601393)

Full Name
P47 Antibody
Gene Names
NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL

CD53, Monoclonal Antibody (Cat# AAA439289)

Full Name
CD53 (TSPAN25) Mouse Monoclonal Antibody
Gene Names
CD53; MOX44; TSPAN25
Reactivity
Human.
Others not tested
Applications
FC/FACS, IF
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (MBS9600182 at 1/100 staining human testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Presenilin 1, Polyclonal Antibody (Cat# AAA9600182)

Full Name
Presenilin 1 Antibody
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data (Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)

Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216585)

Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:Low Endotoxin
Applications
FC/FACS, IF, WB
Pricing
$830/0.5 mg | $3,580/5x0.5 mg

Midkine, Active Protein (Cat# AAA146411)

Full Name
Recombinant Rat Midkine
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
$250/0.02 mg | $2,830/1 mg | $180/0.005 mg | $12,520/5x1 mg

CD14, Monoclonal Antibody (Cat# AAA438726)

Full Name
CD14 (Monocyte / Macrophage Marker) Mouse Monoclonal Antibody
Reactivity
Human.
Others not tested
Applications
FC/FACS, IF
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (MBS9607212 at 1/100 staining Human urothelial cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

S100A8, Polyclonal Antibody (Cat# AAA9607212)

Full Name
S100A8 Antibody
Gene Names
S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data (Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)

DECTIN-1, Monoclonal Antibody (Cat# AAA215797)

Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS, IP
Pricing
$375/0.1 mg | $1,535/5x0.1 mg

CD14, Monoclonal Antibody (Cat# AAA438852)

Full Name
CD14 (Monocyte / Macrophage Marker) Mouse Monoclonal Antibody
Reactivity
Human.
Others not tested
Applications
FC/FACS, IF
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD15 Monoclonal Antibody (BRA-4F1).)

CD15 / FUT4, Monoclonal Antibody (Cat# AAA439616)

Full Name
CD15 / FUT4 (Reed-Sternberg Cell Marker) Mouse Monoclonal Antibody
Gene Names
FUT4; LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV
Reactivity
Human
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)

CD11c, Monoclonal Antibody (Cat# AAA438763)

Full Name
CD11c (Dendritic Cell Marker) Mouse Monoclonal Antibody
Gene Names
ITGAX; CD11C; SLEB6
Reactivity
Human
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunofluorescence (IF) (MBS9608960 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

CCRL2, Polyclonal Antibody (Cat# AAA9608960)

Full Name
CCRL2 Antibody
Gene Names
CCRL2; HCR; CKRX; CRAM; ACKR5; CRAM-A; CRAM-B
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL

Granulocyte Chemotactic Protein 2, Active Protein (Cat# AAA146095)

Full Name
Recombinant Human Granulocyte Chemotactic Protein 2 (CXCL6)
Gene Names
CXCL6; GCP2; CKA-3; GCP-2; SCYB6
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
$250/0.02 mg | $2,830/1 mg | $180/0.005 mg | $12,520/5x1 mg
Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, using LILRA2 antibody., The lane on the left is treated with the antigen-specific peptide.)

LILRA2, Polyclonal Antibody (Cat# AAA9605595)

Full Name
LILRA2 Antibody
Gene Names
LILRA2; ILT1; LIR7; CD85H; LIR-7
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Lymph Node stained with CD14 Mouse Monoclonal Antibody (LPSR/2397).)

CD14, Monoclonal Antibody (Cat# AAA4380320)

Full Name
CD14 (Monocyte/Macrophage Marker)
Reactivity
Human. Others not tested.
Applications
IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB) (Western blot analysis of Annexin A1 antibody (Center) in A2058 cell line lysates (35ug/lane). Annexin A1 (arrow) was detected using the purified Pab.)

Annexin A1, Polyclonal Antibody (Cat# AAA9201730)

Full Name
Annexin A1 Antibody (Center)
Gene Names
ANXA1; ANX1; LPC1
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry (IHC) (MBS9607179 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

CAMP, Polyclonal Antibody (Cat# AAA9607179)

Full Name
CAMP Antibody
Gene Names
CAMP; LL37; CAP18; CRAMP; HSD26; CAP-18; FALL39; FALL-39
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9608319 at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Cathelicidin, Polyclonal Antibody (Cat# AAA9608319)

Full Name
Cathelicidin Antibody
Gene Names
CAMP; LL37; CAP18; CRAMP; HSD26; CAP-18; FALL39; FALL-39
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9601448 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

CD69, Polyclonal Antibody (Cat# AAA9601448)

Full Name
CD69 Antibody
Gene Names
CD69; AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of ANXA1 expression in K562 cells)

ANXA1, Polyclonal Antibody (Cat# AAA9601500)

Full Name
ANXA1 Antibody
Gene Names
ANXA1; ANX1; LPC1
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9601898 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

c-PLA2, Polyclonal Antibody (Cat# AAA9601898)

Full Name
c-PLA2 Antibody
Gene Names
PLA2G4A; cPLA2; PLA2G4; cPLA2-alpha
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Anti-CEACAM Antibody (N-term) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 58 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

CEACAM, Polyclonal Antibody (Cat# AAA9204586)

Full Name
CEACAM Antibody (N-term)
Gene Names
CEACAM1; BGP; BGP1; BGPI
Reactivity
Human
Applications
WB, EIA, IHC-P-Leica, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunofluorescence (IF) (MBS9606663 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

OXER1, Polyclonal Antibody (Cat# AAA9606663)

Full Name
OXER1 Antibody
Gene Names
OXER1; GPCR; GPR170; TG1019
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from K562 cells, using MOX2R antibody.)

MOX2R, Polyclonal Antibody (Cat# AAA9606169)

Full Name
MOX2R Antibody
Gene Names
CD200R1; OX2R; MOX2R; CD200R; HCRTR2
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of LPPL expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)

LPPL, Polyclonal Antibody (Cat# AAA9603899)

Full Name
LPPL Antibody
Gene Names
CLC; GAL10; Gal-10; LGALS10; LGALS10A; LPPL_HUMAN
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data (Growth Regulated Oncogene Beta (GROb) is a small cytokine belonging to the CXC chemokine family which produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. Besides, Dipeptidyl Peptidase IV (DPP4) has been identified as an interactor of GROb, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GROb and recombinant rat DPP4. Briefly, GROb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to DPP4-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GROb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of GROb and DPP4 was shown in Figure 1, and this effect was in a dose dependent manner.)

Growth Regulated Oncogene Beta (GROb), Active Protein (Cat# AAA2097295)

Full Name
Active Growth Regulated Oncogene Beta (GROb)
Gene Names
Cxcl2; Mip-2; Scyb2
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$150/0.01 mg | $255/0.05 mg | $385/0.1 mg | $470/0.2 mg | $895/0.5 mg | $1,315/1 mg | $3,210/5 mg | $14,445/5x5 mg
Immunohistochemistry (IHC) (MBS9601098 at 1/200 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

c-PLA2, Polyclonal Antibody (Cat# AAA9601098)

Full Name
Phospho-c-PLA2 (Ser505) Antibody
Gene Names
PLA2G4A; cPLA2; PLA2G4; cPLA2-alpha
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9607176 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

CYBB, Polyclonal Antibody (Cat# AAA9607176)

Full Name
CYBB Antibody
Gene Names
CYBB; CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with CD81 Rabbit Recombinant Monoclonal Antibody (C81/2885R).)

CD81/TAPA-1, Monoclonal Antibody (Cat# AAA4380751)

Full Name
CD81/TAPA-1
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human, Mouse, Rat. Others not known.
Applications
Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB) (Figure 1. Western blot analysis of Lactoferrin using anti-Lactoferrin antibody (MBS1750451).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates,Lane 2: rat spleen tissue lysates,Lane 3: mouse spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lactoferrin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Lactoferrin at approximately 85KD. The expected band size for Lactoferrin is at 78KD. )

Lactoferrin, Polyclonal Antibody (Cat# AAA1750451)

Full Name
Anti-Lactoferrin Picoband antibody
Gene Names
LTF; LF; HLF2; GIG12; HEL110
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Flow Cytometry (FC/FACS) (Anti-Ly6G staining of murine neutrophils. Ex vivo murine (C57BL6/J) neutrophils from zymosan-elicited peritoneal exudate were stained with anti-Ly6G antibody and analysed by flow-cytometry. Forward-and side-scatter was used to gate on neutrophil population (data not shown) and fluorescence intensity analysed. Left panel shows the signal obtained from cells pre-treated with Fc-blocking reagent and subsequently stained with anti-Ly6G (hybridoma-derived antibody obtained from competitor, clone 1A8,) directly conjugated to PE. Comparison to unstained cells shows that that cells analysed are predominantly Ly6G-positive. Right panel shows staining first with anti-Ly6G or isotype control followed by staining with fluorescently conjugated secondary antibody (goat anti-rat). As a further control, cells were also only stained with secondary antibody. Comparison of the staining pattern obtained using agreeing with findings obtained using hybridoma-derived antibody.)

Ly6G, Monoclonal Recombinant Antibody (Cat# AAA488300)

Full Name
Anti-Ly6G [1A8]
Reactivity
Mouse
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB) (Western blot analysis of CD31 expression in mouse liver)

CD31, Polyclonal Antibody (Cat# AAA9601286)

Full Name
CD31 Antibody
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9606313 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

CCBP2, Polyclonal Antibody (Cat# AAA9606313)

Full Name
CCBP2 Antibody
Gene Names
ACKR2; D6; hD6; CCR9; CCBP2; CCR10; CMKBR9
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL

NAP-2, Active Protein (Cat# AAA692103)

Full Name
Human NAP-2
Gene Names
NAP1L4; NAP2; NAP2L; hNAP2; NAP1L4b
Reactivity
Human
Purity
98 % (SDS-PAGE, HPLC)
Pricing
$395/0.01 mg | $250/0.002 mg | $1,565/5x0.01 mg
Western Blot (WB) (Western blot analysis NFAM1 using NIH-3T3 whole cell lysates)

NFAM1, Polyclonal Antibody (Cat# AAA9609343)

Full Name
NFAM1 Antibody
Gene Names
NFAM1; CNAIP
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FCGR2A protein (Right) or empty vector (Left) detected with FCGR2A antibody)

FCGR2A, Monoclonal Antibody (Cat# AAA532810)

Full Name
FCGR2A antibody
Applications
IF, IHC, WB
Purity
FCGR2A antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB) (Western blot analysis of Phospho-PECAM-1 (Tyr713) Antibody expression in JK cells lysates.The lane on the right is treated with the antigen-specific peptide.)

PECAM-1, Polyclonal Antibody (Cat# AAA9600962)

Full Name
Phospho-PECAM-1 (Tyr713) Antibody
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL

Fc gamma receptor III, Monoclonal Recombinant Antibody (Cat# AAA488349)

Full Name
Anti-Fc gamma receptor III [3G8]
Reactivity
Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Squirrel Monkey, Sooty Mangabey, Cotton-topped Tamarin, Marmoset
Applications
FC/FACS, SPR, IP, IHC, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Activity Data (Figure. The binding activity of GM-CSF with CSF2Ra.Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. It also has some effects on mature cells of the immune system. These include, for example, inhibiting neutrophil migration and causing an alteration of the receptors expressed on the cells surface. GM-CSF signals via signal transducer and activator of transcription, STAT5. In macrophages, it has also been shown to signal via STAT3. Besides, Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra) has been identified as an interactor of GM-CSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GM-CSF and recombinant rat CSF2Ra. Briefly, GM-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CSF2Ra-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GM-CSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of GM-CSF and CSF2Ra was shown in Figure 1, and this effect was in a dose dependent manner.)

Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF), Active Protein (Cat# AAA2105061)

Full Name
Active Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)
Gene Names
Csf2; Gmcsf; Gm-csf
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$175/0.01 mg | $330/0.05 mg | $505/0.1 mg | $620/0.2 mg | $1,195/0.5 mg | $1,775/1 mg | $4,350/5 mg
Western Blot (WB) (RASGRP2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RASGRP2 antibody detected the RASGRP2 protein (arrow).)

RASGRP2, Polyclonal Antibody (Cat# AAA9207263)

Full Name
RASGRP2 Antibody (N-term)
Gene Names
RASGRP2; CDC25L; CALDAG-GEFI
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
WB, EIA, IHC-P, IF
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL

GRO-beta/MIP-2, Active Protein (Cat# AAA144089)

Full Name
Recombinant Rat GRO-beta/MIP-2 (CXCL2)
Gene Names
Cxcl2; Mip-2; Scyb2
Purity
Greater than 99.0% as determined by SDS-PAGE.
Pricing
$250/0.02 mg | $2,830/1 mg | $180/0.005 mg | $12,520/5x1 mg
Western Blot (WB) (Western blot analysis of PECAM-1 expression in Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

CD31, Polyclonal Antibody (Cat# AAA9601794)

Full Name
CD31 Antibody
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of p47 phox in lysates of COS7 , using p47 phox Antibody.)

p47 phox, Polyclonal Antibody (Cat# AAA9611015)

Full Name
p47 phox Antibody
Gene Names
NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
Reactivity
Human
Predicted: Pig, Zebrafish, Bovine, Sheep, Dog, Chicken
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$245/0.05 mL | $305/0.1 mL | $365/0.2 mL | $1,495/5x0.2 mL
Western Blot (WB) (Western blot analysis of MAPK13 expression in nocodazole treated HCT116 cell lysate; PMA-treated Pam212 cell lysate, The lane on the left is treated with the antigen-specific peptide.)

MAPK13, Polyclonal Antibody (Cat# AAA9603875)

Full Name
MAPK13 Antibody
Gene Names
MAPK13; SAPK4; PRKM13; MAPK 13; MAPK-13; p38delta
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of FPRL1 expression in Extracts from HL60 cells.;, The lane on the left is treated with the antigen-specific peptide.)

FPRL1, Polyclonal Antibody (Cat# AAA9604233)

Full Name
FPRL1 Antibody
Gene Names
FPR2; ALXR; HM63; FMLPX; FPR2A; FPRH1; FPRH2; FPRL1; LXA4R; FMLP-R-II
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FCGR2A protein (Right) or empty vector (Left) detected with FCGR2A antibody)

FCGR2A, Monoclonal Antibody (Cat# AAA533685)

Full Name
FCGR2A antibody
Applications
IF, IHC, WB
Purity
FCGR2A antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Testing Data #1 (Human peripheral blood monocytes stained with Mouse anti Human Siglec-5/Siglec-14 (MBS224725) followed by rabbit F(ab')2 anti mouse IgG:FITC (MBS235160))

SIGLEC-5/SIGLEC-14, Monoclonal Antibody (Cat# AAA224544)

Full Name
MOUSE ANTI HUMAN SIGLEC-5/SIGLEC-14:RPE
Gene Names
SIGLEC5; CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Tonsil stained with S100A9 Monoclonal Antibody (S100A9/1011))

S100A9, Monoclonal Antibody (Cat# AAA439010)

Full Name
S100A9 (Macrophage Marker) Mouse Monoclonal Antibody
Gene Names
S100A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
Reactivity
Human.
Others not tested
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Lymph Node stained with CD14 Mouse Monoclonal Antibody (LPSR/2385).)

CD14, Monoclonal Antibody (Cat# AAA4380318)

Full Name
CD14 (Monocyte/Macrophage Marker)
Reactivity
Human. Others not tested.
Applications
IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
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