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Reactivity
Gene Name
Isotype
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Application
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7565 results for "IgG H L Secondary Antibodies" - showing 1-50
Immunohistochemistry (IHC)/Immunofluorescence (IF)
(Validation in COVID-19 Patient Sample. Detection of SARS-CoV-2 proteins in nasopharyngeal swab cell preparations F-H. Co-expression of spike detected by spike antibodies (MBS150780) and envelope proteins detected by envelope antibodies (MBS150849) of SARS-CoV-2 (panel F) documented localization of each protein to glandular cells with negative squamous cells two weeks after full revoery (panel G, signal yellow). No signal was seen in oral swabs of positive cases (Panel H). Both the spike and envelope protein detected by anti-spike antibodies (MBS150780) and anti-envelope antibodies (MBS150849) produced a signal in the nasopharyngeal swabs of the three vases and no signal was evident in the nasopharyngeal swabs of the seven controls.)
COVID 19 Envelope Coronavirus, Polyclonal Antibody (Cat# AAA150849)
Full Name
SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope antibody
Reactivity
Virus
Applications
IF, IHC
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Immunofluorescence (IF)
(Firure 1. Immunofluorescent Validation of MBS150780 in SARS-CoV-2 Infected Lung Tissue (Singh et al., Nature Microbiology, 2021)Multilabel confocal immunofluorescence microscopy of formalin-fixed paraffin-embedded lung sections from rhesus macaques infected with SARS-CoV-2. SARS-CoV-2 spike-specific antibodies, MBS150780. (k, n) (turquoise); Ki67 (magenta) and neutrophil marker CD66abce (yellow) (k); pan-macrophage marker CD68 (magenta) (n) and DAPI (blue).)
COVID 19 Spike Protein Coronavirus, Polyclonal Antibody (Cat# AAA150780)
Full Name
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody
Reactivity
Virus
Applications
IF, IHC, WB
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from Hela, SW480, mouse NIH/3T3 cell line and rat testis tissue lysate(from left to right), using UBE2D2 Antibody (N-term). MBS9213327 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
UBE2D2, Polyclonal Antibody (Cat# AAA9213327)
Full Name
UBE2D2 Antibody (N-term)
Gene Names
UBE2D2; UBC4; PUBC1; UBCH4; UBC4/5; UBCH5B; E2(17)KB2
Reactivity
Human, mouse
Applications
WB, EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(All lanes : Anti-GMCL1 Antibody (Center) at 1:2000 dilutionLane 1: 293T/17 whole cell lysateLane 2: HL-60 whole cell lysateLane 3: mouse testis lysateLane 4: MCF-7 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 59 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
GMCL1, Polyclonal Antibody (Cat# AAA9216192)
Full Name
GMCL1 Antibody (Center)
Gene Names
GMCL1; GCL; GCL1; BTBD13; SPATA29
Reactivity
Human, Mouse
Applications
WB
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Western Blot (WB)
(Figure 1 Western Blot Validation of PD-L1 in HeLa CellsLoading: 15ug of lysates per lane. Antibodies: MBS151123 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
PDL-1, Polyclonal Antibody (Cat# AAA151123)
Full Name
PDL-1 Antibody
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC-P, IF, FC
Purity
PD-L1 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(All lanes : Anti-EN1 (Engrailed 1) Antibody (N-term) at 1:2000 dilutionLane 1: U-87 MG whole cell lysateLane 2: U-2OS whole cell lysate Lane 3: SH-SY5Y whole cell lysateLane 4: 293T/17 whole cell lysateLane 5: HepG2 whole cell lysateLane 6: Mouse brain lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size: 40 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
EN1 (Engrailed 1), Polyclonal Antibody (Cat# AAA9211211)
Full Name
EN1 (Engrailed 1) Antibody (N-term)
Reactivity
Human, Mouse
Applications
WB, EIA, IHC-P, IF
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9601318 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
ZO 1, Polyclonal Antibody (Cat# AAA9601318)
Full Name
ZO 1 Antibody
Gene Names
TJP1; ZO-1
Reactivity
Human, Mouse, Rat, Pig, Monkey
Applications
WB, IHC, IF, ICC, EIA
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 μg of lysates per lane.Antibodies: TACE 1131 (0.5 μg/mL), TACE 22-001 (2 μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
TACE, Polyclonal Antibody (Cat# AAA150091)
Full Name
TACE Antibody
Gene Names
ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, ICC, IF, FC/FACS
Purity
TACE Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation of BACELoading: 15 ug of lysates per lane. Antibodies: BACE (1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane A-C: human brain tis)
BACE, Polyclonal Antibody (Cat# AAA150291)
Full Name
BACE Antibody
Gene Names
BACE1; ASP2; BACE; HSPC104
Reactivity
Human, Mouse
Applications
EIA, ICC, IF, IHC-P, WB
Purity
BACE Antibody is Ion exchange chromatography purified.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Anti-KRAS Antibody (C-term) at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
KRAS, Polyclonal Antibody (Cat# AAA9210256)
Full Name
KRAS Antibody (C-term)
Gene Names
KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
Reactivity
Human, Mouse, Rat
Predicted: Rat
Predicted: Rat
Applications
WB, EIA, IF, FC/FACS
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
(Figure 1 Western Blot Validation of CXCR4 in HeLa CellsLoading: 15 μg of lysates per lane. Antibodies: 1009 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP )
CXCR4, Polyclonal Antibody (Cat# AAA151360)
Full Name
CXCR4 Antibody
Gene Names
CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, ICC, IP, IF, IHC-p, FC
Purity
CXCR4 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA212109)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$785/1 mg | $3,370/5x1 mg
Western Blot (WB)
(Western blot analysis of extracts of mouse pancreas, using FOXN3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
FOXN3, Antibody (Cat# AAA9135025)
Full Name
FOXN3 Polyclonal Antibody
Gene Names
FOXN3; CHES1; PRO1635; C14orf116
Reactivity
Mouse
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot(WB)
(Anti-G6PC Antibody (Center) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution. Predicted band size: 40 kDa . Blocking/Dilution buffer: 5% NFDM/TBST.)
G6PC, Polyclonal Antibody (Cat# AAA9208384)
Full Name
G6PC Antibody (Center)
Gene Names
G6PC; G6PT; GSD1; G6PC1; GSD1a
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF)
(issue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Goat anti-GFP was used at 1:500 dilution in free floating imunnohistochemistry to detect GFP. Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.)
GFP, Antibody (Cat# AAA534795)
Full Name
GFP antibody (biotin)
Reactivity
Assay by immunoelectrophoresis resulted in a single precipitation arc against anti-goat serum, anti-biotin and purified and partially purified green fluorescent protein (Aequorea victoria) Serum. no reaction was observed against Human, Mouse, and Rat ser
Pricing
$1,080/1 mg | $4,855/5x1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation of MyD88 in HeLa (A) and Jurket (B) CellsLoading: 15 μg of lysates per lane.Antibodies: 2125 (1 μg/mL) 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
MYD88, Polyclonal Antibody (Cat# AAA150754)
Full Name
MYD88 Antibody
Gene Names
MYD88; MYD88D
Reactivity
Human, Mouse
Applications
EIA, WB, IHC, IF
Purity
MYD88 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysate from 293 cell line, using RSPO2 Antibody (C-term). MBS9206616 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
RSPO2, Polyclonal Antibody (Cat# AAA9206616)
Full Name
RSPO2 Antibody (C-term)
Gene Names
RSPO2; CRISTIN2
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
WB, IHC-P, FC, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Test Data
IgG (H&L), Secondary Antibody (Cat# AAA688397)
Full Name
Donkey anti-Rabbit IgG (H&L) - Affinity Pure, DyLight350 Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG
Reactivity
Rabbit.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rabbit serum proteins
IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rabbit serum proteins
IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep
Applications
FC/FACS, IF, EIA, IM
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Rabbit IgG.
Affinity purified using solid phase Rabbit IgG.
Pricing
$290/1 mg | $1,145/5x1 mg
Flow Cytometry (FC/FACS)
(Blocking of Fc-receptors by anti-CD16/32 antibody. Ex vivo murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate, left and middle panel) as well as bone marrow-derived macrophages (BMDMs, right panel) were stained with a murine anti-human antibody (fluorescently labelled (AF647) human-specific anti-CD16) with or without pre-incubation with anti-mouse CD16/CD32 (Fc-receptors) antibody (clone 2.4G2). Pre-incubation of cells with anti-CD16/CD32 reduced non-specific binding of the fluorescently labelled antibody to all cell types analysed and shows that this anti-CD16/CD32 antibody acts as an Fc-blocking reagent.)
CD16/CD32, Recombinant Antibody (Cat# AAA488078)
Full Name
Anti-CD16/CD32 [2.4G2]
Gene Names
Fcgr3; CD16
Reactivity
Mouse
Applications
FC/FACS, IF, IP, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western blot analysis of lysate from human kidney tissue lysate, using ACE2(SARS-R) Antibody. MBS9204203 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
ACE2 (SARS Receptor), Polyclonal Antibody (Cat# AAA9204203)
Full Name
ACE2 (SARS Receptor) Antibody (Center)
Gene Names
ACE2; ACEH
Reactivity
Human
Applications
EIA, IHC-P, FC, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Testing Data
(Figure 1 Overexpression Validation of PD-L1 in 293 CellsLoading: 15 μg of lysates per lane.Antibodies: (A, 0.25 μg/mL; B, 0.5 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.)
PDL1, Monoclonal Antibody (Cat# AAA154572)
Full Name
PDL1 Antibody [6H10]
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
EIA, WB, IHC, ICC, IF
Purity
Protein A purified
Pricing
$495/0.1 mg | $2,225/5x0.1 mg
Immunohistochemistry (IHC)
(Figure 5 Immunohistochemistry Validation of ACE2 inHuman Kidney Tissue Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody (3227) at 2ug/ml. Tissue was fixed with formaldehyde and blockedwith 10% serum for 1 h at RT; antigen retrieval was byheat mediation with a citrate buffer (pH6). Samples wereincubated with primary antibody overnight at 4°C. A goatanti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.Counter stained with Hematoxylin.)
ACE2, Polyclonal Antibody (Cat# AAA151315)
Full Name
ACE2 Antibody
Gene Names
ACE2; ACEH
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC-P, IF
Purity
ACE2 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Anti-ITA6 at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 127 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
ITA6, Monoclonal Antibody (Cat# AAA9200194)
Full Name
ITA6 Antibody
Gene Names
ITGA6; CD49f; VLA-6; ITGA6B
Reactivity
Human
Applications
IF, EIA, WB, FC/FACS, IHC-P
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using P2RY12 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)
P2RY12, Polyclonal Antibody (Cat# AAA9135576)
Full Name
P2RY12 Polyclonal Antibody
Gene Names
P2RY12; HORK3; P2Y12; ADPG-R; BDPLT8; SP1999; P2T(AC); P2Y(AC); P2Y(12)R; P2Y(ADP); P2Y(cyc)
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Testing Data
(Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human TissuesLoading: 15 ug of lysates per lane. Antibodies: ACE2, MBS150723 (2 ug/mL), ACE2, MBS150187 (2 ug/mL), ACE2, MBS151315 (2 ug/mL) and beta-actin MBS150383 (1 ug))
ACE2, Polyclonal Antibody (Cat# AAA150723)
Full Name
ACE2 Antibody
Gene Names
ACE2; ACEH
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF
Purity
ACE2 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysate from human placenta tissue lysate, using ALPI Antibody (Center). MBS9201729 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Alkaline Phosphatase (ALPI), Polyclonal Antibody (Cat# AAA9201729)
Full Name
Alkaline Phosphatase (ALPI) Antibody (Center)
Gene Names
ALPI; IAP
Reactivity
Human
Applications
WB, EIA, IHC, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Loading: 15 ?g of lysates per lane. Antibodies: RIP3 (MBS150424), (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution)
RIP3, Polyclonal Antibody (Cat# AAA150424)
Full Name
RIP3 Antibody
Gene Names
Ripk3; Rip3; AW107945; 2610528K09Rik
Reactivity
Human, Mouse, Rat
Applications
ELISA, IF, IHC-P, IP, WB
Purity
RIP3 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of 293T cells, using KAT6A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS9134994) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
KAT6A, Antibody (Cat# AAA9134994)
Full Name
KAT6A Polyclonal Antibody
Gene Names
KAT6A; MOZ; MRD32; MYST3; MYST-3; ZNF220; RUNXBP2; ZC2HC6A
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of lysate from human placenta tissue lysate, using M-CSF Antibody (Center). MBS9203985 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
M-CSF, Polyclonal Antibody (Cat# AAA9203985)
Full Name
M-CSF Antibody (Center)
Gene Names
CSF1; MCSF; CSF-1
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
EIA, FC/FACS, IF, IHC, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 μg of lysates per lane.Antibodies: DR4 1139 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: HepG2 cell lysateLane B: Jurkat cell lysate)
DR4, Polyclonal Antibody (Cat# AAA151363)
Full Name
DR4 Antibody
Gene Names
TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
Reactivity
Human
Applications
EIA, WB, ICC
Purity
DR4 Antibody is Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210328)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. liver section using FGFR2 Antibody (N-term). MBS9208263 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
FGFR2, Polyclonal Antibody (Cat# AAA9208263)
Full Name
FGFR2 Antibody (N-term)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, mouse, rat, monkey
Applications
EIA, IHC, IF, WB, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Testing Data (TD)
( All lanes : Anti-BDNF Antibody (C-term) at 1:1000 dilution Lane 1: human cerebellum lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
BDNF, Polyclonal Antibody (Cat# AAA9211799)
Full Name
BDNF Antibody (C-term)
Gene Names
BDNF; ANON2; BULN2
Reactivity
Human
Predicted Reactivity: Bovine, Horse, Mouse, Pig, Rat
Predicted Reactivity: Bovine, Horse, Mouse, Pig, Rat
Applications
WB, IHC-P, IF, FC, E
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
( All lanes : Anti-ROR1 Antibody at 1:4000 dilutionLane 1: K562 whole cell lysateLane 2: -MB-231 whole cell lysateLane 3: NCI-H226 whole cell lysateLane 4: A549 whole cell lysateLane 5: NIH/3T3 whole cell lysate Lane 6: Mouse kidney tissue lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 104 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
ROR1, Polyclonal Antibody (Cat# AAA9200685)
Full Name
ROR1 Antibody
Gene Names
ROR1; NTRKR1; dJ537F10.1
Reactivity
Human, mouse
Applications
WB, EIA, FC/FACS, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Western Blot (WB)
(Western blot analysis of hsc70 using various lysates.Lanes 1 - 2: Merged signal (red and green). Green - AF5187 observed at 70kDa. Red - loading control, MBS9610328, observed at 55 kDa. Blots were developed with Goat Anti-Rabbit IgG(H+L)FITC–conjugated (MBS9612520) and Goat Anti-Mouse IgG(H+L) AlexaFluor 594–conjugated (MBS9612517) secondary antibodies)
beta-Tubulin, Monoclonal Antibody (Cat# AAA9610328)
Full Name
beta-Tubulin Mouse Monoclonal Antibody
Gene Names
TUBB3; CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
Reactivity
Human, Mouse, Rat, Sheep, Rabbit, Dog, Monkey, Hamster, Chicken
Applications
WB, IHC, IF, ICC
Purity
Peptide affinity purification
Pricing
$190/0.05 mL | $245/0.1 mL | $310/0.2 mL | $855/1 mL | $3,800/5x1 mL
Western Blot (WB)
(JMJD3 Antibody)
JMJD3, Antibody (Cat# AAA150629)
Full Name
JMJD3 Antibody
Gene Names
KDM6B; JMJD3
Reactivity
Human, Mouse, Rat
Applications
EIA, ICC, IF, WB
Purity
JMJD3 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
GAPDH, Polyclonal Antibody (Cat# AAA9205368)
Full Name
GAPDH Antibody (C-term R248)
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. stomach section using Pink1(115-213). MBS9200258 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
PINK1, Monoclonal Antibody (Cat# AAA9200258)
Full Name
PINK1 Antibody
Gene Names
PINK1; BRPK; PARK6
Reactivity
Human
Applications
EIA, IHC, IF, WB
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Western Blot (WB)
(Western blot analysis of lysate from A2058 cell line, using p16INK4a Antibody (C-term E119). MBS9207467 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
p16INK4a, Polyclonal Antibody (Cat# AAA9207467)
Full Name
p16INK4a Antibody (C-term E119)
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
EIA, IHC, FC/FACS, IF, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF)
(Immunofluorescent analysis of GFP using either natural fluorescence (green) or an GFP antibody (red) in Hela(human cervical epithelial adenocarcinoma cell line) cells transfected with GFP recombinant protein. Formalin-fixed cells were permeabilized with 0. 1% Triton X-100 for 10 minutes at room temperature and blocked with 3% BSA for 30 minutes at room temperature. Cells were probed with an GFP monoclonal antibody (Product # MBS9200313) at a dilution of 1:25 for 1 hour at 37°C, and incubated with DyLight 555 goat anti-mouse IgG secondary antibody (Product # please inquire) at a dilution of 1:200 for 60 minutes at 37°C. The nuclear counter stain is DAPI (blue).)
GFP Tag, Monoclonal Antibody (Cat# AAA9200313)
Full Name
GFP Tag Antibody
Reactivity
Human
Applications
IF, EIA, WB
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
$190/0.05 mL | $435/0.2 mL | $1,705/5x0.2 mL
ATF6, Polyclonal Antibody (Cat# AAA150934)
Full Name
ATF6 Antibody
Gene Names
ATF6; ATF6A
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF
Purity
ATF6 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(All lanes : Anti-SOCS1 Antibody (N-term) at 1:2000 dilutionLane 1: mouse thymus lysatesLane 2: rat spleen lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
SOCS1, Polyclonal Antibody (Cat# AAA9210758)
Full Name
SOCS1 Antibody (N-term)
Gene Names
SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
Reactivity
Mouse, rat
Applications
WB, EIA, IHC, IF, FC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of lysates from human placenta tissue lysate,RD cell line (from left to right), using ACTA1/alpha-actin Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
ACTA1/alpha-actin, Polyclonal Antibody (Cat# AAA9231831)
Full Name
ACTA1/alpha-actin Antibody (C-term)
Gene Names
ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM
Reactivity
Human
Predicted: Xenopus, Bovine, Chicken, Mouse, Rabbit, Rat, Pig
Predicted: Xenopus, Bovine, Chicken, Mouse, Rabbit, Rat, Pig
Applications
WB, IHC-P, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from mouse C2C12, rat PC-12 cell line (from left to right), using Bcl-2-BH3 Antibody. MBS9215176 was diluted at 1:500 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Bcl-2, Polyclonal Antibody (Cat# AAA9215176)
Full Name
Bcl-2 Antibody (BH3 Domain Specific)
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
WB, EIA, IHC, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry
(MBS9217260 staining beta Tubulin in mouse thymus tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
beta Tubulin, Polyclonal Antibody (Cat# AAA9217260)
Full Name
beta Tubulin
Gene Names
Tubb5; AA408537; AI596182; M(beta)5; B130022C14Rik
Reactivity
Mouse, Rat; Predicted Reactivity: Bovine, Chicken, Human, Mouse, Pig, Rat
Applications
WB, EIA, FC, IHC-P
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Western Blot (WB)
(Anti-LGR5/GPR49 Antibody (N-term) at 1:1000 dilution + Mouse skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 100 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
LGR5/GPR49, Polyclonal Antibody (Cat# AAA9214227)
Full Name
LGR5/GPR49 Antibody (N-term)
Gene Names
LGR5; FEX; HG38; GPR49; GPR67; GRP49
Reactivity
Mouse (Predicted: Human)
Applications
WB, EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF)
(Immunofluorescent analysis of Human pancreas tissues and Human kidney tissues, using TTR Antibody (C-term). MBS9200982 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).)
TTR, Polyclonal Antibody (Cat# AAA9200982)
Full Name
TTR Antibody (C-term)
Gene Names
TTR; CTS; CTS1; PALB; TBPA; HEL111; HsT2651
Reactivity
Human, Rat
Applications
WB, EIA, IF, IHC-P, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Anti-PLD6 Antibody (Center) at 1:2000 dilution + human brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
PLD6, Polyclonal Antibody (Cat# AAA9204473)
Full Name
PLD6 Antibody (Center)
Gene Names
PLD6; ZUC
Reactivity
Human, Mouse
Applications
WB, EIA
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(All lanes : Anti-Activin A Receptor Type IB(ACVR1B) Antibody (N-term) at 1:1000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lane 3: Rat brain lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Activin A Receptor Type IB (ACVR1B), Polyclonal Antibody (Cat# AAA9201918)
Full Name
Activin A Receptor Type IB (ACVR1B) Antibody (N-term)
Gene Names
ACVR1B; ALK4; SKR2; ACTRIB; ACVRLK4
Reactivity
Human, Mouse (Predicted Reactivity: Rat)
Applications
WB, EIA, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Anti-S100B Antibody at 1:2000 dilution + Human brain whole tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 11 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
S100B, Polyclonal Antibody (Cat# AAA9212013)
Full Name
S100B Antibody
Gene Names
S100B; NEF; S100; S100-B; S100beta
Reactivity
Human, Mouse, Rat (Predicted: Rabbit)
Applications
WB, IHC-P-Leica, FC/FACS, IHC, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
