Rabbit TACE Polyclonal Antibody | anti-ADAM17 antibody

TACE Antibody

Cat. Num. AAA150091

• Gene Names: ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: TACE Antibody is affinity chromatography purified via peptide column.
• Synonyms: TACE; Polyclonal Antibody; TACE Antibody; CSVP; NISBD; ADAM18; CD156B; Disintegrin and metalloproteinase domain-containing protein 17; Snake venom-like protease; ADAM 17; ADAM metallopeptidase domain 17; anti-ADAM17 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: 80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated TACE.
• Purity/Purification: TACE Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 824

• Applicable Applications for anti-ADAM17 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: TACE antibody can be used for detection of TACE by Western blot at 0.5 mug/mL. For immunocytochemistry use 10 mug/mL. For immunofluorescence start at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: TACE antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human TACE.
• Buffer: TACE Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TACE antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 μg of lysates per lane.Antibodies: TACE 1131 (0.5 μg/mL), TACE 22-001 (2 μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

Immunocytochemistry (IHC)

(Figure 4 Immunocytochemistry Validation of TACE in HeLa CellsImmunohistochemical analysis of HeLa cells using anti-TACE antibody (1131) at 10 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

Western Blot (WB)

(Figure 5 KD Validation of TACE in Monkey COS Cells. (Wang et al., 2006)COS cells stably expressing Pref-1A were transfected with control siRNA or TACE siRNA. TACE was detected in lysates by using the anti-TACE antibody (1131). TACE expression levels were markedly reduced in TACE knockdown cell lysate.)

Western Blot (WB)

(Figure 6 KD Validation of TACE in MDA-MB-435 Cells. (McGowan et al., 2007)ADAM-17 protein expression, following transfection with ADAM-17 shRNA (two clones) or neomycin-resistant negative control vector, was examined by immunoblot analysis with anti-ADAM-17 antibodies (1131).)

Western Blot (WB)

(Figure 7 Overexpression Validation of TACE in MCF-7 Cells. (McGowan et al., 2007)ADAM-17 (TACE) protein expression, following transfection of vector and ADAM-17 cDNA, was examined by immunoblot analysis with anti-ADAM-17 (1131) antibodies in MCF-7 cells. Increased ADAM-17 was detected in ADAM-17 transfected cells.)

Western Blot (WB)

(Figure 8 Induced Expression Validation of TACE in Rat Cortical Neurons (Hurtado et al., 2002)Effect of oxygen–glucose deprivation(OGD) or glutamate on the levels of TACE/ADAM17 in rat cortical cultures. Western blot analysis of TACE in homogenates from control, glutamate, and OGD-exposed cultures from a representative experiment.)

Immunofluorescence (IF)

(Figure 9 Immunofluorescence Validation of TACE in Rat Cortical Neurons (Hurtado et al., 2002)Double immunostaining of control and glutamate-exposed rat cortical cultures. (A) Control cultures show TACE immunoreactivity at the cellular membrane of some microglial cells (B) Glutamate-exposed cultures show that most microglial cells express TACE immunoreactivity.(C) Control cultures show that TACE immunostaining does not colocalize with astrocytes [glial fibrillary acidic protein (GFAP)-positive cells]. (D) Astrocyte (GFAP-positive cell) showing TACE immunoreactivity in its surface after treatment with glutamate.)

Immunofluorescence (IF)

(Figure 10 Immunofluorescence Validation of TACE in Rat Brain (Pradillo et al, 2005)Cellular localization of TACE. Double immunofluorescence staining of brain sections from sham-operated (SHAM; A, C, E) and IPC-exposed animals (IPC; B, D, F) of TACE (red) and the cellular markers (green) NeuN (neurons; A, B), GFAP (astrocytes; C, D) and L. esculentum lectin (microglia and endothelium; E, F). White arrows indicate TACE-positive cells.)

Related Product Information for anti-ADAM17 antibody

TACE Antibody: Tumor-necrosis factor-alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-α is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-α. TNF-α converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.

NCBI and Uniprot Product Information

• NCBI GI #: 73747889
• NCBI GeneID: 6868
• NCBI Accession #: NP_003174
• NCBI GenBank Nucleotide #: NM_003183.4
• UniProt Accession #: P78536; O60226
• Molecular Weight: Predicted: 93 kDa
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 17 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 17
• NCBI Official Symbol: ADAM17
• NCBI Official Synonym Symbols: CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 17; TNF-alpha convertase; snake venom-like protease; TNF-alpha converting enzyme; ADAM metallopeptidase domain 18; tumor necrosis factor, alpha, converting enzyme
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 17
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM17
• UniProt Synonym Gene Names: CSVP; TACE; ADAM 17
• UniProt Entry Name: ADA17_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

TACE: a type I membrane protein with disintegrin and metalloprotease activity. An ubiquitously expressed ectoenzyme of peptidase family M12B. Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Possesses a narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha) to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, and the amyloid precursor protein. Also involved in the activation of Notch pathway. Binds 1 zinc ion per subunit. Two splice variant isoforms have been described.

Protein type: Protease; Membrane protein, integral; Motility/polarity/chemotaxis; EC 3.4.24.86

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: focal adhesion; cell surface; membrane; integral to plasma membrane; apical plasma membrane; cytoplasm; plasma membrane; intercellular junction; actin cytoskeleton; lipid raft

Molecular Function: integrin binding; protein binding; interleukin-6 receptor binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; Notch binding; SH3 domain binding; PDZ domain binding

Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; T cell differentiation in the thymus; neutrophil mediated immunity; cell motility involved in cell locomotion; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; proteolysis; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; extracellular matrix disassembly; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; negative regulation of interleukin-8 production; germinal center formation; cell adhesion; epidermal growth factor receptor signaling pathway; response to drug; spleen development; Notch signaling pathway; response to high density lipoprotein stimulus; membrane protein intracellular domain proteolysis; membrane protein ectodomain proteolysis; positive regulation of transforming growth factor beta receptor signaling pathway; Notch receptor processing; positive regulation of cell motility; positive regulation of cell growth; positive regulation of chemokine production; regulation of mast cell apoptosis; collagen catabolic process; PMA-inducible membrane protein ectodomain proteolysis; B cell differentiation; response to hypoxia; cell adhesion mediated by integrin; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; wound healing, spreading of epidermal cells; positive regulation of cell migration

Disease: Inflammatory Skin And Bowel Disease, Neonatal, 1