Rabbit KRAS Polyclonal Antibody | anti-KRAS antibody

KRAS Antibody (C-term)

Cat. Num. AAA9210256

• Gene Names: KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
• Reactivity: Human, Mouse, Rat; Predicted: Rat
• Applications: Western Blot, ELISA, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: KRAS; Polyclonal Antibody; KRAS Antibody (C-term); GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; N-terminally processed; KRAS2; RASK2; anti-KRAS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Rat
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This KRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-174 amino acids from the C-terminal region of human KRAS.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 146-174
• Sequence Length: 188

• Applicable Applications for anti-KRAS antibody: Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:2000; IF~~1:10~50; FC~~1:10~50
• Function: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:20949621). Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed:24623306).
• Cellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytosol
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-KRAS Antibody (C-term) at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-KRAS Antibody (C-term) at 1:500 dilutionLane 1: Hela whole cell lysateLane 2: 293T/17 whole cell lysateLane 3: C2C12 whole cell lysateLane 4: C6 whole cell lysateLane 5: HT-29 whole cell lysateLane 6: PC-12 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDa)

Western Blot (WB)

(All lanes : Anti-KRAS Antibody (C-term) at 1:2000 dilutionLane 1: 293T/17 whole cell lysateLane 2: C2C12 whole cell lysateLane 3: Hela whole cell lysateLane 4: HT-29 whole cell lysateLane 5: K562 whole cell lysate Lane 5: PC-12 whole cell lysateLane 5: Ramos whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of KRAS Antibody (C-term)(MBS9210256) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(KRAS Antibody (C-term)(MBS9210256) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-KRAS antibody

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Product Categories/Family for anti-KRAS antibody

Cancer; Signal Transduction

References

Bruckman, K.C., et al. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 110(5):632-637(2010)
Wong, K.K., et al. Am. J. Pathol. 177(4):1611-1617(2010)
Irahara, N., et al. Diagn. Mol. Pathol. 19(3):157-163(2010)
Carotenuto, P., et al. Pharmacogenomics 11(8):1169-1179(2010)
Leventopoulos, G., et al. Clin. Exp. Rheumatol. 28(4):556-557(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 15718761
• NCBI GeneID: 3845
• NCBI Accession #: NP_004976.2; NP_203524.1
• NCBI GenBank Nucleotide #: NM_004985.4
• UniProt Accession #: P01116; P01118; Q96D10; A8K8Z5; B0LPF9
• NCBI Official Full Name: GTPase KRas isoform b
• NCBI Official Synonym Full Names: Kirsten rat sarcoma viral oncogene homolog
• NCBI Official Symbol: KRAS
• NCBI Official Synonym Symbols: NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
• NCBI Protein Information: GTPase KRas
• UniProt Protein Name: GTPase KRas
• Protein Family: GTPase
• UniProt Gene Name: KRAS
• UniProt Synonym Gene Names: KRAS2; RASK2
• UniProt Entry Name: RASK_HUMAN

NCBI Description

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

Uniprot Description

KRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein; Motility/polarity/chemotaxis; G protein, monomeric; G protein, monomeric, Ras; Oncoprotein

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: extrinsic to internal side of plasma membrane; focal adhesion; membrane; mitochondrion; cytoplasm; plasma membrane; cytosol; lipid raft

Molecular Function: protein binding; GDP binding; GTP binding; GMP binding; protein complex binding; LRR domain binding

Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; nerve growth factor receptor signaling pathway; activation of MAPKK activity; response to glucocorticoid stimulus; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; small GTPase mediated signal transduction; positive regulation of cell proliferation; visual learning; negative regulation of neuron apoptosis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of nitric-oxide synthase activity; MAPKKK cascade; social behavior; response to mineralocorticoid stimulus; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; Ras protein signal transduction; insulin receptor signaling pathway; striated muscle cell differentiation; innate immune response; positive regulation of protein amino acid phosphorylation; blood coagulation; vascular endothelial growth factor receptor signaling pathway; actin cytoskeleton organization and biogenesis; leukocyte migration

Disease: Bladder Cancer; Gastric Cancer, Hereditary Diffuse; Pancreatic Cancer; Noonan Syndrome 3; Leukemia, Acute Myeloid; Breast Cancer; Lung Cancer; Schimmelpenning-feuerstein-mims Syndrome; Cardiofaciocutaneous Syndrome 2