Western Blot (WB)
(Western blot analysis of lysate from 293 cell line, using RSPO2 Antibody (C-term). MBS9206616 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Rabbit RSPO2 Polyclonal Antibody | anti-RSPO2 antibody
RSPO2 Antibody (C-term)
IHC-P~~1:50~100
FC~~1:10~50
Western Blot (WB)
(Western blot analysis of lysate from 293 cell line, using RSPO2 Antibody (C-term). MBS9206616 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysate from 293 cell line, using RSPO2 Antibody (C-term). MBS9206616 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Immunohistochemistry (IHC)
(RSPO2 Antibody (C-term) (Cat. #MBS9206616) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RSPO2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(RSPO2 Antibody (C-term) (Cat. #MBS9206616) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RSPO2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(RSPO2 Antibody (C-term) (MBS9206616) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(RSPO2 Antibody (C-term) (MBS9206616) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Kazanskaya, O., et al. Dev. Cell 7(4):525-534(2004)
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
NCBI and Uniprot Product Information
NCBI Description
R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1; MIM 116806) signaling (Kim et al., 2006 [PubMed 16357527]).[supplied by OMIM, Mar 2008]
Uniprot Description
R-spondin-2: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide; Cell development/differentiation
Chromosomal Location of Human Ortholog: 8q23.1
Cellular Component: extracellular space; cell surface; extracellular region
Molecular Function: heparin binding; G-protein-coupled receptor binding; receptor binding
Biological Process: osteoblast differentiation; embryonic forelimb morphogenesis; Wnt receptor signaling pathway; embryonic hindlimb morphogenesis; bone mineralization
Research Articles on RSPO2
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Product Notes
The RSPO2 rspo2 (Catalog #AAA9206616) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 190-218. The RSPO2 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's RSPO2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC-P); Flow Cytometry (FACS/FC); ELISA (EIA). WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50. Researchers should empirically determine the suitability of the RSPO2 rspo2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RSPO2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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